Chlorhexidine digluconate induces mitotic recombination in diploid cells of Aspergillus nidulans

Abstract

Although, beneficial effects of chlorhexidine, as an antiseptic agent, are reported in the literature, our results revealed that chlorhexidine digluconate, at less levels lowered those used clinically, caused toxic and recombinogenic effects on diploid A. nidulans strain.

Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats

“…In these clinical situations, post-surgical control of the recipient graft area (root surface or extraction site) by an antimicrobial agent is recommended and widely used. With the goal to create an optimal environment for wound repair at the graft recipient site or implant placement site covered by rotated palatal flap, the excisional palatal wound is exposed to the antimicrobial agents, which display both bactericidal activity and potential cytotoxicity (Pucher & Daniel 1992, Kubert et al 1993, Mariotti & Rumpf 1999, Hidalgo & Dominguez 2001, Souza-Junior & Castro-Prado 2005.…”

Antibiotic Resistance of Selected Bacteria after Treatment of the Supragingival Biofilm with Subinhibitory Chlorhexidine Concentrations

“…If such a mutagenic effect of CHX does exist, then antibiotic sensitivity could increase or decrease after CHX treatment. Based on a possible mutagenic effect and the resulting increased gene variation, as already shown by other authors [ 23 ], it can be assumed that the development of cross-resistance is promoted. However, resistance patterns for isolates of the untreated growth controls were also altered.…”

“…Although it has not yet been clarified whether CHX possesses mutagenic effects, CHX was highlighted as being the only bisguanide that showed increased mutagenicity [ 22 ]. Furthermore, different studies have shown an increased mitotic crossing that is over-induced by the presence of CHX [ 23 ] and, in animal experiments, there were changes in cells from the blood and the kidneys after treatment with CHX [ 24 ]. Nevertheless, there are experiments showing that there is negative genotoxic potential CHX [ 25 ].…”

Antimicrobial efficacy of the combination of chlorhexidine digluconate and dexpanthenol

“…The highest dose tested without a mutagenic effect was in the mouse dominant-lethal test 1000 mg/kg/d and in the cytogenetic test in the hamster 250 mg/kg/d [24]. With diploid cells of A. nidulans , a test model for the detection of carcinogenic agents, mitotic recombination were induced by 1.5 and 10 µM CHX (in the case of CHX corresponds to 0.0001343% and 0.00089577% respectively) [25]. An increase in chromosomal aberrations in the bone marrow was induced in the mouse following dermal application of 0.2 mL 0.5% CHx solution in distilled water twice daily for 28 days (50 mg/kg) [26].…”