Chlamydospore development in the absence of protein synthesis in Fusarium solani

Cochrane, Vincent W.; Cochrane, Jean Conn

doi:10.1016/0012-1606(70)90103-x

Cited by 17 publications

(9 citation statements)

References 9 publications

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“…The data revealed that the early phase requires expression of various proteins related to gene transcription and new protein synthesis including small ribosomal subunit protein (XP_661677), eukaryotic initiation factor 4A (XP_660536), poly(A)-binding protein/FabM (XP_661604), AN0641.2, TCTP family protein (XP_658245), glutamine synthetase (XP_661763), mitochondrial acetolactate synthase small subunit (XP_662034), glutamine synthetase (AAK70354), dihydroxy-acid dehydrates (XP_663950), and acetylglutamate kinase (XP_ 682039). This is coincident with previous studies on the effects of protein synthesis inhibitors or temperature-sensitive mutants defective in the germination process on the early stage conidial germination of A. nidulans, N. crassa, and F. solani (Osherov and May, 2000;Loo, 1976;Cochrane and Cochrane, 1970).…”

Section: Discussionsupporting

confidence: 82%

Proteomic analysis of early phase of conidia germination in Aspergillus nidulans

Ahn

Kim

et al. 2010

Fungal Genetics and Biology

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Section: Discussionsupporting

confidence: 82%

Proteomic analysis of early phase of conidia germination in Aspergillus nidulans

Ahn

Kim

et al. 2010

Fungal Genetics and Biology

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“…In addition, genes involved in protein biosynthesis have already been shown to be essential for spore germination in Fusarium solani, N. crassa and A. nidulans (Cochrane and Cochrane 1970;Loo 1976;Osherov and May 2000). We also found two transcripts with a predicted function in protein biosynthesis.…”

Section: Discussionmentioning

confidence: 96%

Identification and quantitative expression analysis of genes that are differentially expressed during conidial germination in Pyrenophora teres

Dilger

Felsenstein²,

Schwarz

2003

Mol Genet Genomics

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Net blotch, caused by Pyrenophora teres, is a common disease of barley ( Hordeum vulgareL.). Two PCR-based differential screening techniques, cDNA-amplified fragment length polymorphism (cDNA-AFLP) and suppression subtractive hybridisation (SSH), were employed to clone cDNA copies of transcripts that are up-regulated during conidial germination. The nucleotide sequences of 35 transcripts were analysed, and the amino acid sequences of their predicted products were compared with entries in databases. Eleven of these clones showed homology to genes from other ascomycetes coding for a transcription factor, two regulatory proteins, a putative transposase, a protein required for the biogenesis of cytochrome C oxidase, a threonine synthase, a probable subunit of a phenylalanine-tRNA synthetase, a subunit of RNA polymerase I, a cation transport protein, a vacuolar ATP synthase subunit, and an RNA processing protein. One conserved hypothetical protein was found and 23 sequences could not be functionally classified. The relative expression of five transcripts at 0, 1, 2, 3, 6, 12 and 24 h after induction of germination was determined by real-time RT-PCR using 18S rRNA as the endogenous reference sequence. All transcripts showed a significant increase in expression during early stages of germination. The maximum change in expression relative to ungerminated conidia ranged between 2.6- and 6-fold. The characterisation of genes involved in biochemical processes during the germination of conidia could be useful for target-specific development of new antifungal agents.

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“…Other studies had highlighted the role of regulatory pathways, including the cAMP/protein kinase A (Fillinger et al 2002;Lafon et al 2005Lafon et al , 2006 and the ras/mitogen-activated protein kinase pathway (Hagag et al 2012). On the other hand, the role of protein accumulation during conidial germination in N. crassa, A. nidulans and F. solani has been investigated using either protein-synthesis inhibitors or temperature-sensitive mutants defective in this process (Cochrane and Cochrane 1970;Hagag et al 2012).…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Proteomic profiling of Botrytis cinerea conidial germination

et al. 2014

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Botrytis cinerea is one of the most relevant plant pathogenic fungi. The first step during its infection process is the germination of the conidia. Here, we report on the first proteome analysis during the germination of B. cinerea conidia, where 204 spots showed significant differences in their accumulation between ungerminated and germinated conidia by two-dimensional polyacrylamide gel electrophoresis and qPCR. The identified proteins were grouped by gene ontology revealing that the infective tools are mainly preformed inside the ungerminated conidia allowing a quick fungal development at the early stages of conidial germination. From 118 identified spots, several virulence factors have been identified while proteins, such as mannitol-1-phosphate dehydrogenase, 6,7-dimethyl-8-ribityllumazine synthase or uracil phosphoribosyltransferase, have been disclosed as a new potential virulence factors in botrytis whose role in pathogenicity needs to be studied to gain new insights about the role of these proteins as therapeutic targets and virulence factors.

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Chlamydospore development in the absence of protein synthesis in Fusarium solani

Cited by 17 publications

References 9 publications

Proteomic analysis of early phase of conidia germination in Aspergillus nidulans

Proteomic analysis of early phase of conidia germination in Aspergillus nidulans

Identification and quantitative expression analysis of genes that are differentially expressed during conidial germination in Pyrenophora teres

Proteomic profiling of Botrytis cinerea conidial germination

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