Chlamydial Serology in Infertile women by Immunofluorescence

Punnonen, R.; Terho, P; Nikkanen, V.; Meurman, Olli

doi:10.1016/s0015-0282(16)44056-2

Cited by 192 publications

(54 citation statements)

References 3 publications

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“…Finally in our study, the results which correlated the type of blockage and the chlamydial seropositivity were similar to those which were obtained in the studies which were conducted by Jain M et al, [19] and Punnoenen et al, [10] with bilateral tubal blockages of 39% and 64.7% respectively, thereby suggesting that the high prevalence of the chlamydial seropositivity could be associated with a bilateral tubal damage.…”

Section: Resultssupporting

confidence: 91%

“…The infection which is caused by C. trachomatis results in the formation of antibodies which are detectable in the serum in infected patients. After the study which was done by Punnonen et al, [10], several studies have demonstrated that tubal factor infertility was significantly associated with the serum antibodies to C. trachomatis, which resulted in infertility [11]. Infertile women with tubal diseases are 2 to 4 times more likely to have elevated antibodies to C trachomatis than either infertile women with normal tubes or pregnant women [12].…”

Section: Introductionmentioning

confidence: 99%

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Association of the Serum Anti-chlamydial Antibodies with Tubal Infertility

Surana

Rastogi²,

Nirwan³

2012

JCDR

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Background: Infertility is increasingly becoming a significant health problem in many areas of the world. The infection which is caused by Chlamydia trachomatis is a major cause of tubal factor infertility secondary to salpingitis. However, the data which pertains to infertility attributed to the C. trachomatis infection is limited in India. Aims:To evaluate the chlamydial infection in women who suffered from infertility and to investigate the possible role of the chlamydia serology as a screening test for tubal infertility.Method: This study was aimed at evaluating the chlamydial infection in fifty women with primary infertility and at investigating the possible role of the chlamydial serology as a screening test for tubal infertility, by the detection of the anti-chlamydial IgM antibodies by using E.L.I.S.A. Setting and Design:The present prospective study was carried out at a tertiary care hospital in north India. Results:In this study, a high seropositivity (60%) for the antichlamydial antibody was observed. 52% females showed bilateral tubal blockage, while the most common site of the blockage was the ampullary portion (36%). Conclusion:These findings highlighted a strong correlation between the tubal factor infertility and the antichlamydial antibodies. It also stressed on the need of the screening of infertile women for C. trachomatis with laboratory investigations, which could provide a rapid and specific diagnosis so that early therapeutic interventions could be instituted.Ashish surAnA, VijAylAtA rAstogi, Prem singh nirWAn

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Association of the Serum Anti-chlamydial Antibodies with Tubal Infertility

Surana

Rastogi²,

Nirwan³

2012

JCDR

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“…The association of serum IgG antibodies to C. trachomatis and tubal pathology is commonly known [20,21]. In the present work, we observed that C. trachomatis antibody positivity in subfertile women with tubal damage did not differ significantly from that of subfertile women without tubal damage (Table 1).…”

Section: Discussionsupporting

confidence: 59%

Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women

Muvunyi

Claeys

Sutter

et al. 2011

J Infect Dev Ctries

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Introduction: Chlamydia antibody testing (CAT) in serum has been introduced as a screening method in the infertility workup. We evaluated the test characteristics of two ELISA tests compared to micro-immunofluorescence tests (MIFs). MIFs are considered the gold standard in the C. trachomatis IgG antibodies detection. We also compared the accuracy of all CAT tests in predicting tubal subfertility, using laparoscopy as a reference. Methodology: Four commercial serological methods were used to analyse 101 serum samples for the presence of C. trachomatis IgG antibodies from patients at the Infertility Clinic of Ghent University Hospital. The diagnostic utility for prediction of tubal infertility of serological methods was evaluated based on patients' medical records. Results: A comparison of the serological assays showed little difference in the major performance characteristics: the sensitivities of all MIFs and ELISAs were 100% for all assays (except the ELISA Vircell, with a sensitivity of 90%), and the specificities ranged from 92% for MIF Ani Labsystems to 98% for the MIF Focus and ELISA Vircell. As compared to laparoscopy data, CAT positivity in subfertile women with tubal damage (n=40) did not significantly differ from that of subfertile women without tubal damage (n=61): Positive predictive values (PPV) of CAT ranged from 53% to 60% and negative predictive values (NPV) ranged from 62% to 64%. Conclusion: evaluated ELISAs are comparable to MIFs in the detection of C. trachomatis IgG antibodies and should be preferred for large serological studies, especially in resource poor settings.

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“…Currently Chlamydia trachomatis (C. trachomatis) is the most common sexually transmitted bacterial pathogen (1). Long-term C. trachomatis infection may result in chronic salpingitis, and may eventually be complicated with tubal infertility and ectopic pregnancy (2,3). The detection of C. trachomatis genital infection to prevent transmission and its spread to the upper reproductive tract is challenging for both clinicians and laboratory workers (4).…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Chlamydia trachomatis infection

Mania‐Pramanik

Potdar

Kerkar

2006

Clinical Laboratory Analysis

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Important progress in the diagnosis of Chlamydia trachomatis (C. trachomatis) includes the development of nucleic acid amplification techniques such as polymerase chain reaction (PCR) and ligase chain reaction (LCR). Commercial kits are available, but they are costly, sporadic in availability, must be imported, and are economically beyond the reach of common people. To overcome this limitation, most research laboratories have standardized their in‐house‐developed PCR methods for diagnosing this infection. However, each laboratory has to spend a great deal of time and money to accomplish this. Published reports do not always elaborate the steps involved in standardizing a test so that it can immediately be reproduced in another setting. In the present study we attempted to elaborate the steps involved in standardizing a sensitive and specific PCR technique followed by hybridization with specific C. trachomatis probe to diagnose this infection in cervical, introital, and urine specimens, and used it to determine the infection rate in a clinical population. J. Clin. Lab. Anal. 20:8–14, 2006. © 2006 Wiley‐Liss, Inc.

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Chlamydial Serology in Infertile women by Immunofluorescence

Cited by 192 publications

References 3 publications

Association of the Serum Anti-chlamydial Antibodies with Tubal Infertility

Association of the Serum Anti-chlamydial Antibodies with Tubal Infertility

Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women

Diagnosis of Chlamydia trachomatis infection

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