Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women

Muvunyi, Claude Mambo; Claeys, Laurens; Sutter, Tineka De; Sutter, Petra De; Temmerman, Marleen; Renterghem, L Van; Claeys, Geert; Padalko, Elizaveta

doi:10.3855/jidc.1740

Cited by 8 publications

(4 citation statements)

References 27 publications

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“…In contrast to our study, Muvunyi et al showed that peptide-based ELISA performed as well as two different IF assays (Muvunyi et al, 2012). However, the performance of the InoDiag tests presented here are consistent with previous studies that revealed good sensitivities and specificities of ELISA assays based on peptides from the MOMP (Muvunyi et al, 2012 ;Land JA et al, 2003).The new automated microarray from Inodiag® show similar performance than ELISA. It is well designed for small laboratories, since batches of only 2 to 4 samples can be tested with InoDiag.…”

Section: Discussionsupporting

confidence: 87%

Performance of an automated multiplex immunofluorescence assay for detection of Chlamydia trachomatis immunoglobulin G

Baud

Zufferey

Hohlfeld

et al. 2014

Diagnostic Microbiology and Infectious Disease

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Chlamydia serology is indicated to investigate etiology of miscarriage, infertility, PID and ectopic pregnancy. Here, we assessed the reliability of a new automated-multiplex immunofluorescence assay (InoDiag test) to detect specific anti-C. trachomatis IgG.Considering IF as gold standard, InoDiag tests exhibited similar sensitivities (65.5%), but better specificities (95.1%-98%) than ELISAs. InoDiag tests demonstrated similar or lower crossreactivity rates when compared to ELISA or IF.

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Section: Discussionsupporting

confidence: 87%

Performance of an automated multiplex immunofluorescence assay for detection of Chlamydia trachomatis immunoglobulin G

Baud

Zufferey

Hohlfeld

et al. 2014

Diagnostic Microbiology and Infectious Disease

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“…The sensitivity and specificity of Chlarnydia IgG antibody test (CAT) are comparable to that of HSG alone. In addition CAT is less cost prohibitive than PCR and has less risks than either HSG or laparoscopy [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Screening for Chlamydia trachomatis in Egyptian women with unexplained infertility, comparing real-time PCR techniques to standard serology tests: case control study

et al. 2015

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BackgroundTo study the prevalence of Chlamydia infection in women with primary and secondary unexplained infertility using ELISA technique for antibody detection and real time, fully automated PCR for antigen detection and to explore its association with circulating antisperm antibodies (ASA).MethodsA total of 50 women with unexplained infertility enrolled in this case control study and a control group of 44 infertile women with a known cause of infertility. Endocervical specimens were collected for Chlamydia antigen detection using PCR and serum samples for antibodies detection. Circulating anti-sperm antibodies were detected using sperm antibody Latex Agglutination tests.ResultsThe overall prevalence of Chlamydial infection in unexplained infertility cases as detected by both ELISA and PCR was 40 % (20/50). The prevalence of current Chlamydial genital infection as detected by real-time PCR was only 6.0 % (3/50); two of which were also IgM positive. Prevalence of ASA was 6.0 % (3/50); all were sero-negative for anti-C.trachomatis IgM and were PCR negative.ConclusionThe incidence of Chlamydial infection in Egyptian patients with unexplained infertility is relatively high. In the setting of fertility investigations; screening for anti. C.trachomatis antibodies using ELISA, and treatment of positive cases should be considered. The presence of circulating ASA does not correlate with the presence of old or current Chlamydia infection in women with unexplained infertility.

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“…[7] It involves use of immunoglobulin G (IgG), A and M detections in either the serum or urine samples. Several studies have confirmed the reliability of using this simple serological test as a predictor of tubal blockage in different settings.…”

Section: Introductionmentioning

confidence: 99%

The prevalence and outcome of asymptomatic chlamydial infection screening among infertile women attending gynecological clinic in Ibadan, South West Nigeria

Morhason-Bello

Ojengbede

Oladokun

et al. 2014

Ann Med Health Sci Res

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Background:Chlamydial trachomatis infection is the most common cause of tubal infertility among women world-wide. Serological diagnosis of Chlamydial infection that may suggest previous, persistent or on-going infection is now incorporated into routine pre-treatment evaluation of infertile women including assisted conception.Aim:The aim of this study is to determine the prevalence and predictors of asymptomatic Chlamydial infection screening among infertile women and also to compare the screening outcome with findings on hysterosalpingogram (HSG).Subjects and Methods:This was an observational study conducted among 132 infertile women that were attending Adeoyo Maternity Hospital Ibadan. A total volume of 2-3 ml of venous blood was collected for Chlamydia serology using ImmunoComb Bivalent immunoglobulin G kit (Code 50416002) and the results were compared with their HSG. Other information collected was socio-demographics and clinical parameters. Descriptive, bivariate and multivariate tests were performed using Statistical Package for the Social Sciences 15.0 (Chicago, IL USA) and statistical significance was set at (P < 0.05).Results:A total of 130 women were studied with a mean age of 31.6 years (standard deviation = 4.7). Majority - 72.0% (95/132) - had been infertile for 5 years or less. The prevalence of Chlamydial trachomatis was 20.5% (27/132). Bivariate analysis between the biosocial variables and serology result showed a significant association with education (P < 0.01) and religion (P < 0.01). Logistic regression analysis revealed that Muslim women were 3.6 times more likely than Christians to have positive Chlamydial serology result (95% confidence interval odds ratio = 1.18-11.11). Of those with HSG result (64), the accuracy of the test kit showed low sensitivity - 44.2% (19/43) and negative predictive value 40.0% (16/40) (but, high specificity - 76.2%(16/21), and positive predictive value - 79.2% (19/24).Conclusion:Asymptomatic Chlamydial infection is common among infertile women and it positively predict HSG blockage. The serological test may prove invaluable in predicting the presence of tubal blockage; therefore, prophylactic antibiotics may be justified to be included in their care.

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Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women

Cited by 8 publications

References 27 publications

Performance of an automated multiplex immunofluorescence assay for detection of Chlamydia trachomatis immunoglobulin G

Performance of an automated multiplex immunofluorescence assay for detection of Chlamydia trachomatis immunoglobulin G

Screening for Chlamydia trachomatis in Egyptian women with unexplained infertility, comparing real-time PCR techniques to standard serology tests: case control study

The prevalence and outcome of asymptomatic chlamydial infection screening among infertile women attending gynecological clinic in Ibadan, South West Nigeria

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