Chlamydial Infections

Schachter, Julius

doi:10.1056/nejm197802232980805

Cited by 506 publications

(204 citation statements)

References 46 publications

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“…Our observations also suggest a low sensitivity for DFA, and indicate that experienced personnel are required for screening. Our own observations and earlier studies revealed that the Pap smear for diagnosing C. trachomatis infection was not sensitive (7)(8)(9) and cannot be recommended under any circumstances for that purpose (10). At present, no method of confirmation for PCR or LCR has been approved for use.…”

Section: Discussionmentioning

confidence: 90%

Diagnosis of Chlamydia trachomatis infection

Mania‐Pramanik

Potdar

Kerkar

2006

Clinical Laboratory Analysis

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Important progress in the diagnosis of Chlamydia trachomatis (C. trachomatis) includes the development of nucleic acid amplification techniques such as polymerase chain reaction (PCR) and ligase chain reaction (LCR). Commercial kits are available, but they are costly, sporadic in availability, must be imported, and are economically beyond the reach of common people. To overcome this limitation, most research laboratories have standardized their in‐house‐developed PCR methods for diagnosing this infection. However, each laboratory has to spend a great deal of time and money to accomplish this. Published reports do not always elaborate the steps involved in standardizing a test so that it can immediately be reproduced in another setting. In the present study we attempted to elaborate the steps involved in standardizing a sensitive and specific PCR technique followed by hybridization with specific C. trachomatis probe to diagnose this infection in cervical, introital, and urine specimens, and used it to determine the infection rate in a clinical population. J. Clin. Lab. Anal. 20:8–14, 2006. © 2006 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 90%

Diagnosis of Chlamydia trachomatis infection

Mania‐Pramanik

Potdar

Kerkar

2006

Clinical Laboratory Analysis

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“…These characteristics identify persons acting as potential reservoirs for bacterial dissemination, with and without clinically apparent manifestations (16) . The use of nucleic acid amplifi cation to detect persistent infections due to C. trachomatis has revealed prevalence rates ranging from 6.5% to 17.5% (17) (18) , which is in agreement with the results described in the present paper and those of studies that performed isolation in cell cultures (10) or serological methods (15) to quantify persistent infections.…”

Section: Discussionmentioning

confidence: 99%

Chlamydia trachomatis serotype A infections in the Amazon region of Brazil: prevalence, entry and dissemination

Ishak

Costa

Almeida

et al. 2015

Rev. Soc. Bras. Med. Trop.

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“…When sexually transmitted it is also a major cause of salpingitis and infertility [2]. Whilst antibiotic treatment of individual cases is effective in both ocular and genital infection, such treatment of 'at risk' communities is not possible.…”

Section: Introductionmentioning

confidence: 99%

Synthetic peptides based on Chlamydia trachomatis antigens identify cytotoxic T lymphocyte responses in subjects from a trachoma-endemic population

Holland

Conway

Blanchard

et al. 1997

Clinical and Experimental Immunology

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SUMMARYCD8 + cytotoxic T lymphocytes (CTL) recognize peptide antigens in the context of class I MHC antigen molecules. To identify peptides capable of eliciting anti-Chlamydia trachomatis CTL responses, 13 synthetic peptides conforming to human leucocyte antigen (HLA)-B8-or -B35-predicted binding motifs were synthesized using sequences based on C. trachomatis major outer membrane protein (MOMP) and heat shock protein 60 (hsp60). Two of 11 HLA-B35-predicted binding peptides were able to stabilize HLA-B35 in an in vitro binding assay. All peptides were tested in CTL assays using peripheral blood mononuclear cells (PBMC) isolated from 26 HLA-B8 or -B35 individuals resident in a trachomaendemic community. Responses to MOMP and hsp60 peptides were identified in a minority of both HLA-B8 and -B35 individuals. Two of 12 HLA-B8 subjects responded to MOMP and 1/13 to hsp60 peptides. Responses in HLA-B35 subjects were similar, 1/13 subjects responding to MOMP and 2/13 to hsp60 peptides. CTL responses were observed only in children resolving current infection and in adults without scarring of the conjunctiva. These results suggest that anti-chlamydial CTL occur at low levels in peripheral blood, but may be important in the resolution of naturally acquired human ocular chlamydial infection.

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Chlamydial Infections

Cited by 506 publications

References 46 publications

Diagnosis of Chlamydia trachomatis infection

Diagnosis of Chlamydia trachomatis infection

Chlamydia trachomatis serotype A infections in the Amazon region of Brazil: prevalence, entry and dissemination

Synthetic peptides based on Chlamydia trachomatis antigens identify cytotoxic T lymphocyte responses in subjects from a trachoma-endemic population

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