Chlamydia trachomatis genovar distribution in clinical urogenital specimens from Tunisian patients: high prevalence of C. trachomatisgenovar E and mixed infections

Gharsallah, Houda; Frikha‐Gargouri, Olfa; Sellami, Hanen; Besbes, Fatma; Znazen, Abir; Hammami, Adnène

doi:10.1186/1471-2334-12-333

Cited by 23 publications

(17 citation statements)

References 39 publications

(31 reference statements)

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“…A total of 107 C. trachomatis ‐positive samples from the microbiology laboratory, Habib Bourguiba, University Hospital of Sfax, were included in this study. Of the 107 samples, 85 were from clinical patients (CP) attending the STD clinics and 22 were from female sex workers (FSW) (Gharsallah, Frikha‐Gargouri, Besbes, et al., ). Urethral and endocervical samples were analyzed through the Cobas Amplicor testing for the detection of C. trachomatis infection.…”

Section: Methodsmentioning

confidence: 99%

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Comparison of reverse hybridization and ompA sequencing methods applied on Chlamydia trachomatis strains from Tunisia

et al. 2017

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Two techniques based on ompA amplification of Chlamydia trachomatis were compared, being reverse hybridization (RHM) and ompA sequencing (OSA), to investigate the concordance between them and to study the epidemiological relevance of each method. In addition, phylogenetic analysis was performed on the ompA sequences. One hundred and seven C. trachomatis positive samples from Tunisian patients and female sex workers were analyzed using both the RHM and ompA sequencing. The overall genovar distribution obtained with both techniques was very similar. The RHM identified nine genovars, being B, D, E, F, G, H, I, J and K, where B, I, J, and K were only found in mixed infections versus 7 types for the OSA being D, E, F, G, H, I, and K. The agreement between both typing techniques was 87.8%. Both methods showed that genovar E was the most predominant type. In 24.3% of the analyzed samples, mixed infections were detected. In 96.1% of these, the genovar identified by OSA was also detected using the RHM. OmpA sequencing allowed determination of six genovar types that could not be typed using RHM. The analyses of ompA nucleotide variation in the 107 clinical specimens detected ompA genovar variants with distinct ompA mutational patterns for types D2, G1, G2, and H1. In conclusion, RHM and OSA showed a high agreement in C. trachomatis genotyping results with each having their specific benefits.

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Section: Methodsmentioning

confidence: 99%

“…The reverse hybridization method, developed in our laboratory, was used for the detection of specific C . trachomatis genovars (Gharsallah, Frikha‐Gargouri, Besbes, et al., , Gharsallah, Frikha‐Gargouri, Sellami, et al, ). Briefly, the nylon membrane was spotted with probes hybridizing specifically to genovars A to L3 at specific positions.…”

Section: Methodsmentioning

confidence: 99%

Comparison of reverse hybridization and ompA sequencing methods applied on Chlamydia trachomatis strains from Tunisia

et al. 2017

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“…Urogenital C. trachomatis infection has become a major public health problem world-wide and is one of the most frequent causes of sexually transmitted diseases (1,2). It is a common cause of urethritis and cervicitis.…”

Section: Discussionmentioning

confidence: 99%

“…Chlamydia trachomatis (C. trachomatis) is an obligate intra-cellular pathogen and the most frequent sexually transmitted bacterium worldwide (1)(2)(3)(4). Most infections occur without symptoms and asymptomatic manifestation of urogenital chlamydial infection can be observed in ~30% of patients.…”

Section: Introductionmentioning

confidence: 99%

Frequency of Chlamydia trachomatis in Endocervical Samples of Women Referred to a Gynecology Hospital in Qazvin, Iran

Aslanimehr¹,

Ghazvini²,

Saadat³

et al. 2015

Biotech Health Sci

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Background:Chlamydia trachomatis (C. trachomatis) is the most common bacterial sexually transmitted infection (STI). Although most genital C. trachomatis infections remain asymptomatic but infection with these bacteria is the leading cause of complications, such as pelvic inflammatory disease (PID), tubal factor infertility and abortion. Objectives: The objective of this study was to estimate the frequency of C. trachomatis infection among symptomatic and asymptomatic women, by a polymerase chain reaction (PCR) based assay. Patients and Methods: This was a cross-sectional study conducted over the period from May 2012 to February 2013. A total of 240 nonduplicate endocervical samples were taken from married women; endocervical swabs were collected from women referred to Qazvin Kowsar Gynecology Hospital by a training midwife. The specimens were tested for C. trachomatis by a PCR-based assay for the pha gene. Results: Out of the 240 female participants, 184 (76.7%) were symptomatic and 56 (23.3%) were asymptomatic cases. The mean age of cases was 37.1 ± 0.9 years. Twenty (8.3%) of the 240 samples were diagnosed as Chlamydia positive according to PCR results. The prevalence of asymptomatic C. trachomatis infections was six (10.7%), while there were 14 (7.6%) in symptomatic cases. Although positive PCR results have shown in women with vaginosis (7.1%), abortion (5.1%), premature birth and low birth weight below 2.5 kg (7.7%) but the chi-square test did not indicate a significant relationship between positive PCR test results and these symptoms. Conclusions:The results of this study showed that there was a high prevalence of C. trachomatis infection among both symptomatic and asymptomatic women. Therefore, a screening test for C. trachomatis infection is recommended for all women who refer to the genitourinary medicine clinic. Screening programs are important for cost effectiveness calculations of C. trachomatis infections especially in asymptomatic cases.

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“…Moreover, genovar E is the most prevalent in other regions of the world and responsible for 40 % of urogenital infection in heterosexual patients [8][9][10].…”

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Chlamydia trachomatis genotypes in school adolescents, Italy

et al. 2015

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Chlamydia trachomatis genogroups using ompA and multilocus sequence typing (MLST) were determined in consecutive isolates from school students aged 18 or older in the district of Brescia, Italy, 2012-2013. Among 40 samples, 4 ompA genovars and 18 STs were identified. Genovar E predominated (70 %) including five STs derived from ST59 (29 % of all isolates). This study, combining ompA and MLST typing of C. trachomatis school teenagers, suggests limited mixing and sexual interchange in this population.

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Chlamydia trachomatis genovar distribution in clinical urogenital specimens from Tunisian patients: high prevalence of C. trachomatisgenovar E and mixed infections

Cited by 23 publications

References 39 publications

Comparison of reverse hybridization and ompA sequencing methods applied on Chlamydia trachomatis strains from Tunisia

Comparison of reverse hybridization and ompA sequencing methods applied on Chlamydia trachomatis strains from Tunisia

Frequency of Chlamydia trachomatis in Endocervical Samples of Women Referred to a Gynecology Hospital in Qazvin, Iran

Chlamydia trachomatis genotypes in school adolescents, Italy

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