Chlamydia pneumoniae and Chronic Skin Wounds: A Focused Review

King, Lloyd E.; Stratton, Charles W.; Mitchell, William M.

doi:10.1046/j.0022-202x.2001.00050.x

Cited by 11 publications

(4 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Especially, insufficient prerequisites to avoid contamination, application of techniques highly prone to amplicon carryover, such as the nested PCR format, or even other techniques requiring handling of amplicons appear to be a major problem (2,19,20). The potential problems with contamination were recognized shortly after the introduction of PCR as a diagnostic technique in 1986 (23).…”

Section: We Know the Problem: What Can We Do About It?mentioning

confidence: 99%

“…Based on various NAATs, C. pneumoniae has also been associated with cyclosporine-induced hypergingivismus (37), chronic skin wounds (20), age-related macular degeneration (17), and chronic anemia (27), to mention only a few. As these conditions all have markedly different pathologies and pathogenic mechanisms, to ascribe them to one microorganism is not biologically plausible.…”

Section: Wanted: Etiologic Agentsmentioning

confidence: 99%

See 1 more Smart Citation

In-House Nucleic Acid Amplification Assays in Research: How Much Quality Control Is Needed before One Can Rely upon the Results?

2005

View full text Add to dashboard Cite

3Over the last 20 years, nucleic acid amplification tests (NAATs) have become a major tool for detection of microorganisms, for diagnostic testing, and for research purposes in the field of infectious diseases. NAATs offer significant sensitivity and speed compared to culture and do not require viable organisms. However, validated, commercially available, U.S. Food and Drug Administration-cleared assays exist for the following microorganisms: Mycobacterium tuberculosis, Chlamydia trachomatis, Neisseria gonorrhoeae, methicillin-resistant Staphylococcus aureus, group B streptococcus, Legionella pneumophila, human immunodeficiency virus, hepatitis B virus, and hepatitis C virus. Some of these tests are for very limited indications, for example, the methicillin-resistant Staphylococcus aureus assay is intended only for use with nasopharyngeal swabs as an infection control tool.There are also a number of so-called analyte-specific reagents commercially available for clinically relevant pathogens and pathogenicity factors like herpes simplex virus, EpsteinBarr virus, cytomegalovirus, Streptococcus pyogenes, and Bordetella pertussis and the genes for vanA/vanB and mecA, respectively. Next to these relatively closed and standardized kit concepts, the use of NAATs for research purposes has expanded dramatically. These assays range from those that are well validated to not validated at all, yet these assays are frequently used and cited in the literature. A review of the current literature on the association of a particular microorganism and a particular disease frequently reveals inconsistent results, even apparently when the same methods are used.Although NAATs offer the promise of exquisite sensitivity, theoretically allowing for detection of a single organism in a clinical sample, both false-negative and -positive results can and do occur. There can be problems with sensitivity, specificity, and contamination, which can be secondary to a very large number of technical issues, as listed in Tables 1 to 6. Some of the more common problems in context with NAAT-based studies of microorganisms and disease associations are described below. FALSE-POSITIVE RESULTS DUE TO CONTAMINATIONThe possible association between human herpesvirus 8 (HHV-8) and a direct role in Kaposi's sarcoma pathogenesis is very important. The presence of this potentially oncogenic virus in semen donated for use in artificial insemination would be critically important to know. However, the prevalences of HHV-8 DNA in prostate and semen as measured by NAATs range from 0 to over 90% (32). A recent study (32) assessed interlaboratory sensitivity and reproducibility in the analysis of blinded, identical experimental panels, each consisting of 48 samples composed of semen specimens from artificial insemination donors, human immunodeficiency virus-infected patients, and positive and negative controls. As no commercial, validated assay is available, each laboratory followed its standard procedures for HHV-8 PCR; thus, procedures varied from laboratory to labora...

show abstract

Section: We Know the Problem: What Can We Do About It?mentioning

confidence: 99%

Section: Wanted: Etiologic Agentsmentioning

confidence: 99%

In-House Nucleic Acid Amplification Assays in Research: How Much Quality Control Is Needed before One Can Rely upon the Results?

2005

View full text Add to dashboard Cite

show abstract

“…Species of the genus Chlamydia are associated with chronic infections in humans including infections of the urogenital tract, eyes and lungs (AdderleyKelly & Stephens, 2005;Beagley & Timms, 2000;Bébéar & de Barbeyrac, 2009;Kayes et al, 2007). Recent findings demonstrate that Chlamydia species are opportunistic pathogens that can be detected in chronic skin ulcers and other inflammatory skin conditions (King et al, 2001). The Chlamydia life cycle includes two forms: infectious, metabolically inert, extracellular elementary bodies (EBs); and vegetative, intracellular, reticulate bodies (RBs).…”

Section: Introductionmentioning

confidence: 99%

Non-thermal argon plasma is bactericidal for the intracellular bacterial pathogen Chlamydia trachomatis

Sа

Sysolyatina

Колкова

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

Non-thermal plasma (NTP) is a flow of partially ionized argon gas at an ambient macroscopic temperature and is microbicidal for bacteria, viruses and fungi. Viability of the Gram-negative obligate intracellular bacterial parasite Chlamydia trachomatis and its host cells was investigated after NTP treatment. NTP treatment of C. -fold reduction in the concentration of infectious bacteria. When the samples were covered with magnesium fluoride glass to obstruct plasma particles and UV rays alone were applied, the bactericidal effect was reduced 1.4¾10 1 -fold and 5¾10 4-fold for EBs and RBs, respectively. NTP treatment caused the viability of host McCoy cells to diminish by 19 %. Therefore, the results obtained demonstrated that (i) both extracellular and intracellular forms of C. trachomatis are sensitive to NTP treatment; (ii) the reduction in concentration of infectious bacteria after NTP treatment of infected cells is superior to the reduction in viability of host cells; and (iii) the effect of NTP on intracellular bacteria does not depend on UV rays.

show abstract

“…The reason for this assertion is that C. pneumoniae is not only the germ causing the frequent respiratory infections, but that it can bring about processes which originally used to be a�ributed solely to C. trachomatis (reactive arthritis, ocular, genital and dermatologic infections) (Peeling and Brunham, 1996;Thomsen et al, 1996;Bernhard et al, 2001;King et al, 2001). Furthermore the C. pneumoniae infection represents another risk factor for the development of atherosclerosis and also its destabilization, and because it can be a co-factor of the origin of the bronchopulmonal carcinoma and some other chronic diseases of the central nervous system (sclerosis multiplex etc.)…”

mentioning

confidence: 99%

Chlamydia (Chlamydophila) pneumoniae in animals: a review

Pospíśil¹,

Čanderle²

2004

Vet. Med. - Czech

View full text Add to dashboard Cite

An important discovery in the last couple of years is that humans are not the only natural hosts with which C. pneumoniae is the primary cause for the disease. Successively, the C. pneumoniae strain was isolated from horses, koala bears affected by ocular and genital infection, Australian and African frogs, from a Tanzanian chameleon, a green sea turtle living in the Cayman Islands, an iguana, puff adders and a Burmese python. All of the animals in which the C. pneumoniae was confirmed, were suffering from some form of illness that is also typical in humans when affected by this chlamydial species. All strains also showed a high similarity with the human C. pneumoniae strain (up to 100%).

show abstract

Chlamydia pneumoniae and Chronic Skin Wounds: A Focused Review

Cited by 11 publications

References 88 publications

In-House Nucleic Acid Amplification Assays in Research: How Much Quality Control Is Needed before One Can Rely upon the Results?

In-House Nucleic Acid Amplification Assays in Research: How Much Quality Control Is Needed before One Can Rely upon the Results?

Non-thermal argon plasma is bactericidal for the intracellular bacterial pathogen Chlamydia trachomatis

Chlamydia (Chlamydophila) pneumoniae in animals: a review

Contact Info

Product

Resources

About