Chitosan-thioglycolic acid conjugate: a new scaffold material for tissue engineering?

Kast, Constantia E.; Frick, Wolfram; Losert, Udo; Bernkop‐Schnürch, Andreas

doi:10.1016/s0378-5173(03)00076-0

Cited by 92 publications

(58 citation statements)

References 13 publications

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“…By alteration of the surface functionality controlled biochemical interactions with body fluids can be achieved. Thiolated chitosan, a biodegradable conjugate obtained by different chemical coupling approaches, combines a series of interesting functions such as mucoadhesive [109], permeation-enhancing [110], in situ gelling and enzyme inhibition properties [111,112]. This conjugate was further processed into functional nanoscale films/coatings built using a layer-by-layer approach for alternate deposition of oppositely charged polyelectrolytes [113].…”

Section: Next Generation Wound Dressings and Formulations Combining Pmentioning

confidence: 99%

Polymers in Wound Repair

Francesko

Fernandes

Rocasalbas

et al. 2014

Advanced Polymers in Medicine

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Efficient dermal wound care implies providing a healing environment at the site of injury. Current repair techniques, including polymeric dressings, are able to accelerate only the healing of epidermal and partial thickness acute wounds based on maintaining the area moist. However, these are not efficient in treatment of full-thickness and chronic wounds, which lack in inborn regenerative elements and are highly prone to infections. For this reason the research interest is nowadays shifted towards functional biomaterials to tackle severe skin deteriorations by providing a beneficiary for healing pro-active and pathogen-free environment. Recent advances in molecular biology and materials science together with better understanding of wound pathophysiology allowed for designing of new wound care approaches that rely on biochemical stimuli to promote wound closure. Biopolymers that couple intrinsic antimicrobial and wound repair properties with hydrophilicity appear as suitable dressing platforms. These can be further upgraded using various bio-entities (therapeutic molecules, cells) with the ability to address specific targets in the biochemical environment of wounds in order to stimulate the healing process. This chapter summarises the abundant experimental and clinical data on polymers in advanced wound dressings, scaffolds for dermal regeneration and platforms for drug delivery.

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Section: Next Generation Wound Dressings and Formulations Combining Pmentioning

confidence: 99%

Polymers in Wound Repair

Francesko

Fernandes

Rocasalbas

et al. 2014

Advanced Polymers in Medicine

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“…Although chitosan and chitosan derivatives have been widely formulated into nanoparticles for drug delivery (12)(13)(14)(15), fabrication of chitosan microspheres (CMs) was not actively studied because deprotonated amine groups of chitosan decrease water solubility in neutral environment (16). Most CMs have been prepared by emulsification and spray drying techniques.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Chitosan Microspheres for Complete Encapsulation of Anionic Proteins: Controlling Particle Size and Encapsulation Efficiency

et al. 2013

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Abstract. Electrospinning was employed to fabricate chitosan microspheres by a single-step encapsulation of proteins without organic solvents. Chitosan in acetic acid was electrospun toward a grounded sodium carbonate solution at various electric potential and feeding rates. Electrospun microspheres became insoluble and solidified in the sodium carbonate solution by neutralization of chitosan acetate. When the freeze-dried microspheres were examined by scanning electron microscopy, the small particle size was obtained at higher voltages. This is explained by the chitosan droplet size at the electrospinning needle was clearly controllable by the electric potential. The recovery yield of chitosan microspheres was dependent on the concentration of chitosan solution due to the viscosity is the major factor affecting formation of chitosan droplet during curling of the electrospinning jets. For protein encapsulation, fluorescently labeled bovine serum albumin (BSA) was codissolved with chitosan in the solution and electrospun. At higher concentration of sodium carbonate solution and longer solidification time in the solution, the encapsulation efficiency of the protein was confirmed to be significantly high. The high encapsulation efficiency was achievable by instant solidification of microspheres and electrostatic interactions between chitosan and BSA. Release profiles of BSA from the microspheres showed that the protein release was faster in acidic solution due to dissolution of chitosan. Reversed-phase chromatography of the released fractions confirmed that exposure of BSA to acidic solution during the electrospinning did not result in structural changes of the encapsulated protein.

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“…The results of this study showed that thiolated chitosan can provide a porous scaffold structure guaranteeing cell anchorage, proliferation and tissue formation in three dimensions 50 . Due to the in situ gelling properties, it seems possible to provide a certain shape of the scaffold material by pouring a liquid thiolated chitosan 51 cell suspension in a mold. Furthermore, liquid polymer cell suspensions may be applied by injection forming semi-solid scaffolds at the site of tissue damage.…”

Section: Production Of Micro-and Nano-particlesmentioning

confidence: 99%