Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes

Lahiji, Ashkan; Sohrabi, Afshin; Hungerford, David S.; Frondoza, Carmelita G.

doi:10.1002/1097-4636(20000915)51:4<586::aid-jbm6>3.3.co;2-j

Cited by 127 publications

(157 citation statements)

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“…For example, researchers have used polysaccharide-based hydrogels, includeing agarose, chitosan and alginates (Suh and Matthew, 2000), as culture substrates. Chitosan is a partially deacetylated product of chitin that has film-forming properties, mimics the natural environment found in the living articular cartilage matrix, and has been shown to help chondrocytes maintain their rounded cell shape when used as a culture substrate (Lahiji et al, 2000;Suh and Matthew, 2000).…”

Section: Morphological Transition Is Essential For the Differentiatiomentioning

confidence: 99%

TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

et al. 2011

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Vertically aligned, laterally spaced nanoscale titanium nanotubes were grown on a titanium surface by anodization, and the growth of chondroprogenitors on the resulting surfaces was investigated. Surfaces bearing nanotubes of 70 to 100 nm in diameter were found to trigger the morphological transition to a cortical actin pattern and rounded cell shape (both indicative of chondrocytic differentiation), as well as the up-regulation of type II collagen and integrin β4 protein expression through the down-regulation of Erk activity. Inhibition of Erk signaling reduced stress fiber formation and induced the transition to the cortical actin pattern in cells cultured on 30-nm-diameter nanotubes, which maintained their fibroblastoid morphologies in the absence of Erk inhibition. Collectively, these results indicate that a titanium-based nanotube surface can support chondrocytic functions among chondroprogenitors, and may therefore be useful for future cartilaginous applications.

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Section: Morphological Transition Is Essential For the Differentiatiomentioning

confidence: 99%

TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

et al. 2011

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“…Previous reports have shown conflicting results about the effects of chitosan on fibroblasts. While chitosan has been shown to have stimulatory effect on fibroblast activity 25,43 , other results have shown some inhibitory effects of chitosan on fibroblasts growth 44,45 . Maybe, sample preparation or different culture conditions could explain these discrepant results.…”

Section: Cell Morphology By Light Microscopymentioning

confidence: 99%

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

2015

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Chitosan is soluble in diluted acid solutions and can easily form films by casting. However, residual acid neutralization should be performed for biomedical applications what may compromise physical and mechanical properties of the films. Thus, plasticizers can be added to improve these properties. The aim of this study was to characterize morphological, barrier and mechanical properties, besides evaluate the in vitro cytotoxicity of sorbitol-plasticized and NaOH-Na 2 CO 3 neutralized chitosan membranes for skin substitute application. Scanning electron microscopy, X-ray diffraction, water vapor permeability and mechanical tests were carried out to characterize the obtained membranes. Moreover, Vero cells were used for in vitro cytotoxicity evaluation. In this paper, we report a non-cytotoxic sorbitol-plasticized chitosan membrane with desirable properties for skin substitution, such as flexibility, water vapor permeability and high percentage of elongation.

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“…28 One way to overcome the heterogeneity in natural matrices uses polymers to fabricate degradable 3D porous matrices. Scaffolds generated from natural polymers such as alginates, [29][30][31] chitosan, [32][33][34][35][36][37][38] collagen, 39 GAGs and elastin, [40][41][42][43] gelatin [44][45][46] and fibrin [47][48][49] have also been used as scaffolding materials. [50][51][52] A commonly used system is collagen/GAGs; 53,54 collagen/GAG based skin equivalents are already in clinical use 41,42 and under investigation for other applications such as heart valves, vascular grafts [55][56][57][58][59][60] and vascular networks.…”

Section: Basics Of Porous Structuresmentioning

confidence: 99%

Cell colonization in degradable 3D porous matrices

Lawrence

Madihally

2008

Cell Adhesion & Migration

175

155

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Cell colonization is an important in a wide variety of biological processes and applications including vascularization, wound healing, tissue engineering, stem cell differentiation and biosensors. During colonization porous 3D structures are used to support and guide the ingrowth of cells into the matrix. In this review, we summarize our understanding of various factors affecting cell colonization in three-dimensional environment. The structural, biological and degradation properties of the matrix all play key roles during colonization. Further, specific scaffold properties such as porosity, pore size, fiber thickness, topography and scaffold stiffness as well as important cell material interactions such as cell adhesion and mechanotransduction also influence colonization.

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Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes

Cited by 127 publications

References 0 publications

TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

Cell colonization in degradable 3D porous matrices

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Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes

Cited by 127 publications

References 0 publications

TiO2nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

TiO2nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

Cell colonization in degradable 3D porous matrices

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Product

Resources

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TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity