Chitosan-shelled oxygen-loaded nanodroplets abrogate hypoxia dysregulation of human keratinocyte gelatinases and inhibitors: New insights for chronic wound healing

Oxygen‐Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP‐9 and TIMP‐1 by Human Monocytes

“…Indeed, OLNs are reportedly able to release significant and clinically relevant amounts of oxygen into hypoxic environments in a time-sustained manner, opposite to OSS, which releases oxygen only transiently, and to OFNs, not releasing oxygen at all [ 16 , 17 ]. In particular, 10% v/v OLNs, OFNs, and OSS have been comparatively challenged for their ability to release oxygen into Panserin 601 cell culturing medium and monitored by oxymetry for 24 h. Although both OLNs and OSS induced an immediate peak in oxygen release, only OLNs released high oxygen amounts in a time-sustained manner for all the observational 24 h-period, whereas OSS effect quickly vanished after a few hours and OFNs did not release significant amounts of oxygen [ 36 ]. These data appear encouraging, since they suggest that a topical administration of multiple doses (e.g., twice a day) of OLNs might be effective.…”

‘In Vitro’, ‘In Vivo’ and ‘In Silico’ Investigation of the Anticancer Effectiveness of Oxygen-Loaded Chitosan-Shelled Nanodroplets as Potential Drug Vector

“…Chitosan-shelled/decafluoropentane-cored oxygen-loaded nanodroplets (OLN), having average diameters of about 700 nm, positive charge and long-term stability (7) have been designed as potential therapeutic tools to promote healing processes in infected chronic wounds, since chitosan-based OLN displayed antimicrobial properties against methylcillin-resistant Staphylococcus aureus and Candida albicans (11). Interestingly, this types of OLN showed the capability to overcome hypoxia-dependent dysregulation of matrix-associated proteolytic enzymes such as matrix metalloproteinases (12).…”

Topical oxygen therapy & micro/nanobubbles: a new modality for tissue oxygen delivery

“…At the cellular level, nanobubbles not only counteract the hypoxia‐dependent dysregulation of the MMP/TIMP balance in human keratinocytes but also reduce HIF‐1‐alpha signalling, both of which have important implications in wound healing. This may relate to the intermittent application of the technology that prevents a switch off of the hypoxic drive induced by a constant flow of oxygen . In addition, through florescent labelling, researchers show that nanobubbles are internalised by cells, including keratinocytes, with a diffuse cytoplasmic distribution .…”

“…This may relate to the intermittent application of the technology that prevents a switch off of the hypoxic drive induced by a constant flow of oxygen . In addition, through florescent labelling, researchers show that nanobubbles are internalised by cells, including keratinocytes, with a diffuse cytoplasmic distribution . Furthermore, studies looking into potential adverse effects of MNBs found no associated cellular toxicity with their application …”

“…In addition, through florescent labelling, researchers show that nanobubbles are internalised by cells, including keratinocytes, with a diffuse cytoplasmic distribution . Furthermore, studies looking into potential adverse effects of MNBs found no associated cellular toxicity with their application …”