‘In Vitro’, ‘In Vivo’ and ‘In Silico’ Investigation of the Anticancer Effectiveness of Oxygen-Loaded Chitosan-Shelled Nanodroplets as Potential Drug Vector

Khadjavi, Amina; Stura, Ilaria; Prato, Maurizio; Minero, Valerio Giacomo; Panariti, Alice; Rivolta, Ilaria; Gulino, Giulia Rossana; Bessone, Federica; Giribaldi, Giuliana; Quaglino, Elena; Cavalli, Roberta; Guiot, Caterina

doi:10.1007/s11095-018-2371-z

Cited by 17 publications

(18 citation statements)

References 41 publications

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“…Chitosan NPs exhibit outstanding biodegradable and biocompatible properties, and for these reasons they are extensively studied as drug carriers. Studies have shown that chitosan NPs can deliver many drugs, including genes, proteins [66,67,68], anticancer chemical drugs [69,70], and antibiotics [71,72]; and via various routes of administration, including oral, nasal, intravenous, and ocular [73,74,75]. CS–TPP NPs have been investigated for the delivery of anticancer and protein drugs by oral administration [76,77,78].…”

Section: Polysaccharide Are Commonly Investigated As Nanoparticulamentioning

confidence: 99%

The Microfluidic Technique and the Manufacturing of Polysaccharide Nanoparticles

et al. 2018

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The microfluidic technique has emerged as a promising tool to accelerate the clinical translation of nanoparticles, and its application affects several aspects, such as the production of nanoparticles and the in vitro characterization in the microenvironment, mimicking in vivo conditions. This review covers the general aspects of the microfluidic technique and its application in several fields, such as the synthesis, recovering, and samples analysis of nanoparticles, and in vitro characterization and their in vivo application. Among these, advantages in the production of polymeric nanoparticles in a well-controlled, reproducible, and high-throughput manner have been highlighted, and detailed descriptions of microfluidic devices broadly used for the synthesis of polysaccharide nanoparticles have been provided. These nanoparticulate systems have drawn attention as drug delivery vehicles over many years; nevertheless, their synthesis using the microfluidic technique is still largely unexplored. This review deals with the use of the microfluidic technique for the synthesis of polysaccharide nanoparticles; evaluating features of the most studied polysaccharide drug carriers, such as chitosan, hyaluronic acid, and alginate polymers. The critical assessment of the most recent research published in literature allows us to assume that microfluidics will play an important role in the discovery and clinical translation of nanoplatforms.

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Section: Polysaccharide Are Commonly Investigated As Nanoparticulamentioning

confidence: 99%

The Microfluidic Technique and the Manufacturing of Polysaccharide Nanoparticles

et al. 2018

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“…The production of nanocarriers is one the most innovative and promising research fields in modern pharmaceutical technology: As a matter of fact, the use of nanoscale formulations can improve drug compatibility [1,2], provide more accurate diagnosis [3,4], reduce the risk of immune response [5,6], and achieve targeted drug delivery [7,8]. Despite the aforementioned scientific advancements, the market of nanoscale medical devices is still a niche one, not only because of regulatory issues, but also because of the difficulties encountered in producing nanocarriers at an industrial scale [9].…”

Section: Introductionmentioning

confidence: 99%

Overcoming the Limits of Flash Nanoprecipitation: Effective Loading of Hydrophilic Drug into Polymeric Nanoparticles with Controlled Structure

et al. 2018

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Flash nanoprecipitation (FNP) is a widely used technique to prepare particulate carriers based on various polymers, and it was proven to be a promising technology for the industrial production of drug loaded nanoparticles. However, up to now, only its application to hydrophobic compounds has been deeply studied and the encapsulation of some strongly hydrophilic compounds, such as caffeine, remains a challenge. Caffeine loaded poly-ε-caprolactone (PCL) nanoparticles were produced in a confined impinging jet mixer using acetone as the solvent and water as the antisolvent. Caffeine was dissolved either in acetone or in water to assess the effects of two different process conditions. Nanoparticles properties were assessed in terms of loading capacity (LC%), encapsulation efficiency (EE%), and in vitro release kinetics. Samples were further characterized by dynamic light scattering, scanning electron microscopy, X-ray photo electron spectroscopy, and infrared spectroscopy to determine the size, morphology, and structure of nanoparticles. FNP was proved an effective technique for entrapping caffeine in PCL and to control its release behavior. The solvent used to solubilize caffeine influences the final structure of the obtained particles. It was observed that the active principle was preferentially adsorbed at the surface when using acetone, while with water, it was embedded in the matrix structure. The present research highlights the possibility of extending the range of applications of FNP to hydrophilic molecules.

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“…Various NDs with polymer shells have been fabricated with the development of polymer materials. The most often used polymer materials include natural polymers such as chitosan [7,8] and alginate [9], as well as synthetic block polymer materials such as poly (ethylene glycol)-block-Poly (ε-caprolactone) (PEG-PCL) [10,11]. Due to the stability and stiffness of the shell, polymeric NDs usually have a higher cavitation threshold and longer circulation time than lipid NDs [12].…”

Section: The Shells Of Ndsmentioning

confidence: 99%

Advances in Targeted Tumor Diagnosis and Therapy Based on Ultrasound-Responsive Nanodroplets

Li¹,

Liu²,

Duan³

et al. 2020

Advanced Ultrasound in Diagnosis and Therapy

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The ultrasound contrast agents currently used in clinics are microbubbles with a large particle size and short circulation time, and their approved clinical applications are limited to endovascular diagnosis and therapy only. The development of ultrasound-responsive nanodroplets (NDs) provides a new approach for extravascular diagnosis and therapy, especially for molecular imaging and targeted therapy of tumors. The NDs with a nano-scaled particle size and a liquid core can maintain their shape and initial diameter during injection, enhancing their EPR effects and facilitating the accumulation of NDs at the tumor site. When exposed to ultrasound, NDs can vaporize and exhibit contrast enhancement at the sites of interest. In addition, the destruction of microbubbles can provide a driving force to facilitate the release of drugs or genes from the microbubbles into target cells, allowing the NDs to act as drug carriers. The development of ultrasound-responsive NDs has shown rapid progress in recent years, while a variety of NDs with excellent properties have been fabricated for targeted diagnosis and drug delivery. In this article, the development of ultrasound-responsive NDs was reviewed in terms of their structure, phase transition properties, and applications in targeted tumor diagnosis and therapy.

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‘In Vitro’, ‘In Vivo’ and ‘In Silico’ Investigation of the Anticancer Effectiveness of Oxygen-Loaded Chitosan-Shelled Nanodroplets as Potential Drug Vector

Abstract: Due to their intrinsic toxicity OLN might be considered an adjuvant tool suitable to deliver their therapeutic cargo intracellularly and may be proposed as promising combined delivery system.

Cited by 17 publications

References 41 publications

The Microfluidic Technique and the Manufacturing of Polysaccharide Nanoparticles

The Microfluidic Technique and the Manufacturing of Polysaccharide Nanoparticles

Overcoming the Limits of Flash Nanoprecipitation: Effective Loading of Hydrophilic Drug into Polymeric Nanoparticles with Controlled Structure

Advances in Targeted Tumor Diagnosis and Therapy Based on Ultrasound-Responsive Nanodroplets

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