Chitosan scaffolds with enhanced mechanical strength and elastic response by combination of freeze gelation, photo-crosslinking and freeze-drying

Silvestro, Ilaria; Sergi, Riccardo; d’Abusco, Anna Scotto; Mariano, Alessia; Martinelli, Andrea; Piozzi, Antonella; Francolini, Iolanda

doi:10.1016/j.carbpol.2021.118156

Cited by 20 publications

(14 citation statements)

References 40 publications

(44 reference statements)

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“…8 On the contrary, the covalent bonds formed during crosslinking in photo-cross-linkable hydrogels render high stability to the polymer network. 20 These findings are consistent with our results. Fig.…”

Section: Resultssupporting

confidence: 93%

GelMA/κ-carrageenan double-network hydrogels with superior mechanics and biocompatibility

Gan

Sun

et al. 2023

RSC Adv.

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Section: Resultssupporting

confidence: 93%

GelMA/κ-carrageenan double-network hydrogels with superior mechanics and biocompatibility

Gan

Sun

et al. 2023

RSC Adv.

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“…Bioactive agents such as calcium phosphates and biologically active biomolecules such as bone morphogenetic protein-2 (BMP-2) [3][4][5] and osteogenic peptide (OP) [6] can be loaded into/onto scaffolds to provide osteoconductivity and osteoinductivity, eliciting desirable cellular responses from seed cells such as osteoprogenitors and mesenchymal stem cells (MSCs) for accelerated bone regeneration. Comparing to dense bone repair biomaterials, porous bone tissue engineering scaffolds mimicking the natural bone ECM can provide better substrates for cell attachment, morphogenesis, proliferation and differentiation and have been fabricated using a variety type of techniques, such as particle/salt leaching [7], gas foaming [8], emulsion freezing/freeze drying [9], phase separation [10] and electrospinning [11]. However, these techniques have challenges in accurately controlling the architecture (porosity, pore size, interconnectivity, shape, etc) and properties (physical and mechanical) of bone scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Low temperature hybrid 3D printing of hierarchically porous bone tissue engineering scaffolds with in situ delivery of osteogenic peptide and mesenchymal stem cells

Lai

Wang²,

Liu³

et al. 2022

Biofabrication

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Comparing to other conventional fabrication techniques, 3D printing is advantageous in producing bone tissue engineering scaffolds with customized shape, tailored pore size/porosity, required mechanical properties and even desirable biomolecule delivery capability. However, towards scaffolds with a large volume, it is highly difficult to enable seed cells to migrate to the central region of the scaffolds, resulting in an inhomogeneous cell distribution, and hence lowering the bone forming ability. To address this problem, in this investigation, cell-laden bone tissue engineering scaffolds consisting of osteogenic peptide loaded β-tricalcium phosphate/poly(lactic-co-glycolic acid) (OP/TCP/PLGA) nanocomposite scaffolds and rat bone marrow derived mesenchymal stem cells (rBMSCs)-laden Gelatin/GelMA hydrogel fillers were produced through a “dual-nozzle” cryogenic hybrid 3D printing. The cell-laden scaffolds exhibited a bi-phasic structure and were mechanically similar to human cancellous bone. OP can be released from the hybrid scaffolds in a sustained manner. rBMSCs laden in the hydrogel patterns exhibited a high viability during and after cryogenic hybrid 3D printing process and can be further released from the hydrogel struts and achieve cell anchorage on the surface of adjacent OTP struts. The OP released from OTP struts enhanced rBMSCs proliferation. Comparing to rBMSC-laden hybrid scaffolds without OP incorporation, the rBMSC-laden hybrid scaffolds incorporated with OP significantly up-regulated the osteogenic differentiation of rBMSCs, by showing a higher level of alkaline phosphatase (ALP) expression and calcium deposition. This “proof-ofconcept” study provided a facile method to form cell-laden bone tissue engineering scaffolds with not only required mechanical strength, biomimetic structure and prolonged biomolecule release but also excellent cell delivery capability with uniform cell distribution.

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“…Its benefits derive from being biodegradable, non-toxic, biocompatible, and bioactive [ 11 , 12 ]. These properties, combined with the advantage of having many functional groups on its backbone (-OH and NH2), have made CS particularly interesting for many applications such as tissue engineering [ 6 , 7 , 8 , 13 , 14 , 15 ], bioremediation [ 16 , 17 , 18 ], agriculture [ 19 ], cosmetics [ 20 ] and as sorbent materials [ 21 , 22 , 23 ] for a wide range of contaminants. CS possesses a suitable reactivity to be modified by chemical or physical processes with the aim of widening its use.…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polymers Based on Chitosan for 2,4-Dichlorophenoxyacetic Acid Removal

Silvestro

Ciarlantini

Francolini

et al. 2022

IJMS

Self Cite

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The development of low-cost and eco-friendly materials for the removal of pollutants from water is one of the main modern challenges. For this purpose, molecularly imprinted polymers were prepared under optimized conditions starting from chitosan (CS), chemically or ionically modified with glycidyl methacrylate (GMA) or itaconic acid (ITA), respectively. 2,4-Dichlorophenoxyacetic acid (2,4-D) was used as a template, obtaining the CS_GMA and CS_ITA series. The influence of the template concentration on the MIPs’ (molecularly imprinted polymers) morphology, thermal behaviour and swelling ability, as well as on the 2,4-D removal capacity, were analyzed. The amount of the template used for the imprinting, together with the different permeability of the matrices, were the key factors driving the analyte uptake process. Despite the good performance shown by the non-imprinted CS_GMA sample, the best results were obtained when CS_GMA was imprinted with the highest amount (5%) of template (CS_GMA_5). This system was also more efficient when consecutive adsorption experiments were carried out. In addition, CS_GMA_5 had a desorption efficiency of 90–100% when a low pesticide concentration was used. These findings suggest that the presence of imprinted cavities could be useful in improving the performance of sorbent materials making CS_GMA_5 a possible candidate for 2,4-D removal.

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Chitosan scaffolds with enhanced mechanical strength and elastic response by combination of freeze gelation, photo-crosslinking and freeze-drying

Cited by 20 publications

References 40 publications

GelMA/κ-carrageenan double-network hydrogels with superior mechanics and biocompatibility

GelMA/κ-carrageenan double-network hydrogels with superior mechanics and biocompatibility

Low temperature hybrid 3D printing of hierarchically porous bone tissue engineering scaffolds with in situ delivery of osteogenic peptide and mesenchymal stem cells

Molecularly Imprinted Polymers Based on Chitosan for 2,4-Dichlorophenoxyacetic Acid Removal

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