Chitosan/Polyurethane Blended Fiber Sheets Containing Silver Sulfadiazine for Use as an Antimicrobial Wound Dressing

Abstract

Electrospun chitosan (CTS) nanofibers have been well known for use as a wound dressing in the biomedical field. Nevertheless, fatal bacterial infections are still a serious problem when CTS nanofibers are used for wound treatment. In this study, we designed a novel wound dressing based on blending the chitosan with polyurethane (CTS/PU) containing silver sulfadiazine (AgSD) in order to enhance both antibacterial activity and mechanical strength. This fiber sheet was produced using the electrospinning (ELSP) technique. The CTS/PU containing AgSD fiber sheet was characterized by energy-dispersive X-ray spectroscopy (EDX). The physicochemical properties of the CTS/PU/AgSD fiber sheets were also characterized by thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FT-IR). The electrospun fibers were morphologically characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). For an in vitro evaluation, the CTS/PU/AgSD fiber sheets were tested for their antibacterial activity against gram-negative Pseudomonas aeruginosa (P. aeruginosa), gram-positive Staphylococcus aureus (S. aureus) and Methicillin-resistant Staphylococcus aureus (MRSA). The results indicate that CTS/PU/AgSD fiber sheets have strong antimicrobial activity as displayed by inhibition of bacterial growth and prevention of infection during the healing process. These results indicate that this material would be good for use as a wound dressing material.

Polymers for medical and tissue engineering applications

“…This is topped by the possibility of processing chitosan and chitosan-composites into numerous shapes; membranes, sponges, fibres, microspheres and hydrogels. Some recent research into chitosan-based scaffolds are aimed at further improving surface characteristics of scaffolds for hard tissue regeneration, 44 exploring its anti-bacterial qualities when combined with other polymers and anti-bacterial agents, 45 increasing stability of chitosan-based cartilage repair scaffolds by co-polymerization with other natural polymers, 46 and introducing chitosan microspheres embedded in calcium sulphate as point-of-care drug delivery systems. 47 Bovine derived collagen, much like the innovative uptake of fungi-derived chitosan, can be used as an example by which solutions for the abovementioned issues can be found, paving the way for the safe and effective future use of this material.…”

In vitro and in vivo assessments of an optimal polyblend composition of polycaprolactone/gelatin nanofibrous scaffolds for Achilles tendon tissue engineering

“…The fabrication of highly aligned PCL/GE ENs was performed as previously described with some modification [11,12,[14][15][16][17][18][19][20]. Briefly, PCL and GE compounds at different blending ratios (10:0, 9:1, 7:3, 5:5) were dissolved in HFIP to produce a 10% (w/v) solution.…”

Overview of Electrospinned Chitosan Nanofiber Composites for Wound Dressings

“…The use of metal nanoparticles had been shown to have high antimicrobial activity against bacteria, viruses, and other microorganisms [76]. Lee et al [73] reported higher efficiency against S. aureus in chitosan/polyurethane nanofibers with silver sulfadiazine. Silver is known for its great antimicrobial properties and its use for pharmaceutical applications.…”