Chitosan/poly(dl,lactide-co-glycolide) scaffolds for tissue engineering

Martel-Estrada, Santos-Adriana; Olivas–Armendáriz, I.; Martínez-Pérez, Carlos A.; Hernández, Tony; Acosta-Gómez, Eduardo I.; Chacón-Nava, J.G.; Jiménez-Vega, Florinda; García-Casillas, Perla E.

doi:10.1007/s10856-012-4762-8

Cited by 13 publications

(8 citation statements)

References 32 publications

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“…These results suggest that the ALP activity of the cells increases proportionally with the percentage of MWCNT composites, this being indicative of osteoblast differentiation [25] [26]. Figure 5 shows the images on the 14 days of culture, observing the similar structure before culture once hy- [27], in Ch/PLLA-2% MWCNT (Figure 5(f)), Ch/PLLA-5%MWCNT (Figure 5(i)) and Ch/PLLAz10%MWCNT (Figure 5(l)). The image shows that the scaffold containing MWCNT have a positive influence on osteoblasts, promoting cell adhesion and spreading.…”

Section: Alkaline Phosphatase Activitymentioning

confidence: 99%

Development of Chitosan/Poly(L-Lactide)/Multiwalled Carbon Nanotubes Scaffolds for Bone Tissue Engineering

Orozco¹,

Jiménez-Vega²,

Martel-Estrada³

et al. 2016

OJRM

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This work focuses to improve the mechanical properties and investigates the growth of osteoblasts on a degradable chitosan/poly(L-lactide)/carbon nanotubes composite for tissue engineering. The morphological and mechanical properties characterizations were performed using scanning electronic microscopy (SEM) and rheometrics analysis system (RSA). Osteoblasts differentiation was determined by alkaline phosphatase activity, total number cells by an Alamar Blue assay, cell attachment and proliferation were visualized qualitatively using SEM, mineralization was characterized by transformed infrared spectroscopy, energy dispersive spectroscopy, and X-ray diffraction. The results suggest that the chitosan/poly(L-lactide)/multiwalled carbon nanotubes composites exhibit the ability to promote cell adhesion, proliferation, and differentiation on their surface.

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Section: Alkaline Phosphatase Activitymentioning

confidence: 99%

Development of Chitosan/Poly(L-Lactide)/Multiwalled Carbon Nanotubes Scaffolds for Bone Tissue Engineering

Orozco¹,

Jiménez-Vega²,

Martel-Estrada³

et al. 2016

OJRM

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“…3,4 In this research, we leverage the experimental, analytical and computational techniques to investigate the feasibility of using shape memory polymers (SMPs) and focused ultrasound (FU) for designing a CDD system. The design is used in a conceptual novel mechanism for simultaneously opening the drug container and pushing the particles out, which will signicantly improve the rate of drug release at a targeted location.…”

Section: Introductionmentioning

confidence: 99%

“…3,52,54,55 By turning off the ultrasound beam the shape recovery process can be paused at any time, which allows the polymer to have an intermediate but a stable shape.…”

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confidence: 99%

Focused ultrasound actuation of shape memory polymers; acoustic-thermoelastic modeling and testing

et al. 2017

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a Controlled drug delivery (CDD) technologies have received extensive attention recently. Despite recent efforts, drug releasing systems still face major challenges in practice, including low efficiency in releasing the pharmaceutical compounds at the targeted location with a controlled time rate. We present an experimentally-validated acoustic-thermoelastic mathematical framework for modeling the focused ultrasound (FU)-induced thermal actuation of shape memory polymers (SMPs). This paper also investigates the feasibility of using SMPs stimulated by FU for designing CDD systems. SMPs represent a new class of materials that have gained increased attention for designing biocompatible devices. These polymers have the ability of storing a temporary shape and returning to their permanent or original shape when subjected to external stimuli such as heat. In this work, FU is used as a trigger for noninvasively stimulating SMP-based systems. FU has a superior capability to localize the heating effect, thus initiating the shape recovery process only in selected parts of the polymer. The multiphysics model optimizes the design of a SMP-based CDD system through analysis of a filament as a constituting basestructure and quantifies its activation under FU. Experimental validations are performed using a SMP filament submerged in water coupled with the acoustic waves generated by a FU transducer. The modeling results are used to examine and optimize parameters such as medium properties, input power and frequency, location, geometry and chemical composition of the SMP to achieve favorable shape recovery of a potential drug delivery system.

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“…Chitosan can be chemically modified under alkaline conditions to yield water soluble carboxymethyl chitosan (CMCS) . CMCS is currently used in tissue engineering, drug delivery, wound dressing, and food industry …”

Section: Introductionmentioning

confidence: 99%

“…[6] CMCS is currently used in tissue engineering, drug delivery, wound dressing, and food industry. [7,8] Hydrogels are generally obtained by crosslinking a hydrophilic polymer. [9] They are able to absorb large amounts of water without dissolution, maintaining their original three-dimensional network structure.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of novel carboxymethyl chitosan grafted polylactide hydrogels for controlled drug delivery

Wang

Zhu

et al. 2015

Polym. Adv. Technol.

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Novel carboxymethyl chitosan‐polylactide (CMCS‐g‐PLA) hydrogels were prepared by using 1‐(3‐dimethylaminopropyl)‐3‐ethylcarbodiimide hydrochloride/N‐hydroxysuccinimide (EDC/NHS) as crosslinking agent and catalyst at room temperature. Solid‐state 13C‐NMR, SEM, and FT‐IR measurements showed that PLA blocks are successfully grafted onto the CMCS main chains. DSC measurements confirmed the effective crosslinking of carboxymethyl chitosan. With increasing the amount of EDC/NHS, the crosslink destiny of CMCS‐g‐PLA copolymers is improved. The swelling ratio of CMCS‐g‐PLA hydrogels is pH dependent, showing a minimum in the pH range of 3 to 5. Rheological studies confirmed the formation of hydrogels. The higher the crosslinking density, the higher the storage modulus of hydrogels. CMCS‐g‐PLA hydrogels only slightly degrade in PBS for 10 days. In the presence of lysozyme, however, hydrogels with low crosslink density are totally degraded in 10 days. Drug release studies show that after 96 h, 95% of thymopentin is released under in vitro conditions. Copyright © 2015 John Wiley & Sons, Ltd.

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Chitosan/poly(dl,lactide-co-glycolide) scaffolds for tissue engineering

Cited by 13 publications

References 32 publications

Development of Chitosan/Poly(L-Lactide)/Multiwalled Carbon Nanotubes Scaffolds for Bone Tissue Engineering

Development of Chitosan/Poly(L-Lactide)/Multiwalled Carbon Nanotubes Scaffolds for Bone Tissue Engineering

Focused ultrasound actuation of shape memory polymers; acoustic-thermoelastic modeling and testing

Synthesis and characterization of novel carboxymethyl chitosan grafted polylactide hydrogels for controlled drug delivery

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