Chitosan oligosaccharides inhibit TNF-α-induced VCAM-1 and ICAM-1 expression in human umbilical vein endothelial cells by blocking p38 and ERK1/2 signaling pathways

Liu, Hongtao; Li, Wenming; Huang, Pei Hsin; Chen, Wenjuan; Liu, Qishun; X, Bai; Du, Yifeng

doi:10.1016/j.carbpol.2010.01.054

Cited by 15 publications

(11 citation statements)

References 32 publications

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“…These mediators activate epithelial cells to release additional chemokines and chemotactic factors, such as vascular adhesion molecule (VCAM) and intercellular adhesion molecule (ICAM), which stimulate the migration of neutrophils to the target tissues, such as the liver, lungs and kidneys [29,30]. A number of previous studies have shown that different molecular weights of chitosan have a range of antiinflammatory actions, including inhibition of the production of TNF-α, IL-6, prostaglandin E2 (PGE2), cyclooxygenase-2 (COX-2), VCAM-1 and ICAM-1 in vitro [17,[31][32][33]. The data we presented here demonstrate that chitosan oligosaccharides significantly inhibit the production of systemic levels of proinflammatory cytokines TNF-α and IL-1β in vivo.…”

Section: Discussionmentioning

confidence: 99%

Chitosan oligosaccharides protect mice from LPS challenge by attenuation of inflammation and oxidative stress

Qiao

2011

International Immunopharmacology

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154

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Section: Discussionmentioning

confidence: 99%

Chitosan oligosaccharides protect mice from LPS challenge by attenuation of inflammation and oxidative stress

Qiao

2011

International Immunopharmacology

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154

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“…CDAs, like PgtCDA, thus emerge as a powerful tool for the production of defined paCOS that cannot currently be produced in any other way. These paCOS can be further analyzed for their structurefunction relationships and used in various applications, such as plant protection or biomedicine, based on their biological activities (4,76).…”

Section: Figmentioning

confidence: 99%

A Recombinant Fungal Chitin Deacetylase Produces Fully Defined Chitosan Oligomers with Novel Patterns of Acetylation

Naqvi

Cord‐Landwehr

Singh

et al. 2016

Appl Environ Microbiol

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Partially acetylated chitosan oligosaccharides (paCOS) are potent biologics with many potential applications, and their bioactivities are believed to be dependent on their structure, i.e., their degrees of polymerization and acetylation, as well as their pattern of acetylation. However, paCOS generated via chemical N-acetylation or de-N-acetylation of GlcN or GlcNAc oligomers, respectively, typically display random patterns of acetylation, making it difficult to control and predict their bioactivities. In contrast, paCOS produced from chitin deacetylases (CDAs) acting on chitin oligomer substrates may have specific patterns of acetylation, as shown for some bacterial CDAs. However, compared to what we know about bacterial CDAs, we know little about the ability of fungal CDAs to produce defined paCOS with known patterns of acetylation. Therefore, we optimized the expression of a chitin deacetylase from the fungus Puccinia graminis f. sp. tritici in Escherichia coli. The best yield of functional enzyme was obtained as a fusion protein with the maltose-binding protein (MBP) secreted into the periplasmic space of the bacterial host. We characterized the MBP fusion protein from P. graminis (PgtCDA) and tested its activity on different chitinous substrates. Mass spectrometric sequencing of the products obtained by enzymatic deacetylation of chitin oligomers, i.e., tetramers to hexamers, revealed that PgtCDA generated paCOS with specific acetylation patterns of A-A-D-D, A-A-D-D-D, and A-A-D-D-D-D, respectively (A, GlcNAc; D, GlcN), indicating that PgtCDA cannot deacetylate the two GlcNAc units closest to the oligomer's nonreducing end. This unique property of PgtCDA significantly expands the so far very limited library of well-defined paCOS available to test their bioactivities for a wide variety of potential applications. IMPORTANCEWe successfully achieved heterologous expression of a fungal chitin deacetylase gene from the basidiomycete Puccinia graminis f. sp. tritici in the periplasm of E. coli as a fusion protein with the maltose-binding protein; this strategy allows the production of these difficult-to-express enzymes in sufficient quantities for them to be characterized and optimized through protein engineering. Here, the recombinant enzyme was used to produce partially acetylated chitosan oligosaccharides from chitin oligomers, whereby the pronounced regioselectivity of the enzyme led to the production of defined products with novel patterns of acetylation. This approach widens the scope for both the production and functional analysis of chitosan oligomers and thus will eventually allow the detailed molecular structure-function relationships of biologically active chitosans to be studied, which is essential for developing applications for these functional biopolymers for a circular bioeconomy, e.g., in agriculture, medicine, cosmetics, and food sciences.

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“…Both ferulic acid and glycol chitosan have neuroprotective effects, such as antioxidation and -inflammation. [16][17][18][19][20][21][22] . The analgesic, buprenorphine (0.05-0.10 mg/kg), was administered every 12 h through subcutaneous injection for the first 3 days postsurgery for postoperation pain management.…”

Section: Methodsmentioning

confidence: 99%

Assessment of White Matter Loss Using Bond-Selective Photoacoustic Imaging in a Rat Model of Contusive Spinal Cord Injury

Wang

Cheng

et al. 2014

Journal of Neurotrauma

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White matter (WM) loss is a critical event after spinal cord injury (SCI). Conventionally, such loss has been measured with histological and histochemical approaches, although the procedures are complex and may cause artifact. Recently, coherent Raman microscopy has been proven to be an emerging technology to study de-and remyelination of the injured spinal cord; however, limited penetration depth and small imaging field prevent it from comprehensive assessments of large areas of damaged tissues. Here, we report the use of bond-selective photoacoustic (PA) imaging with 1730-nm excitation, where the first overtone vibration of CH 2 bond is located, to assess WM loss after a contusive SCI in adult rats. By employing the first overtone vibration of CH 2 bond as the contrast, the mapping of the WM in an intact spinal cord was achieved in a label-free three-dimensional manner, and the physiological change of the spinal cord before and after injury was observed. Moreover, the recovery of the spinal cord from contusive injury with the treatment of a neuroprotective nanomedicine ferulic-acid-conjugated glycol chitosan (FA-GC) was also observed. Our study suggests that bond-selective PA imaging is a valuable tool to assess the progression of WM pathology after SCI as well as neuroprotective therapeutics in a label-free manner.

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Chitosan oligosaccharides inhibit TNF-α-induced VCAM-1 and ICAM-1 expression in human umbilical vein endothelial cells by blocking p38 and ERK1/2 signaling pathways

Cited by 15 publications

References 32 publications

Chitosan oligosaccharides protect mice from LPS challenge by attenuation of inflammation and oxidative stress

Chitosan oligosaccharides protect mice from LPS challenge by attenuation of inflammation and oxidative stress

A Recombinant Fungal Chitin Deacetylase Produces Fully Defined Chitosan Oligomers with Novel Patterns of Acetylation

Assessment of White Matter Loss Using Bond-Selective Photoacoustic Imaging in a Rat Model of Contusive Spinal Cord Injury

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