Chitosan nanoparticles synthesis caught in action using microdroplet reactions

Kamat, Vivek; Bodas, Dhananjay; Paknikar, Kishore M.

doi:10.1038/srep22260

Cited by 51 publications

(31 citation statements)

References 11 publications

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“…However, as seen before, the strongest factor influencing the average hydrodynamic diameter of the particles was chitosan concentration. It should be noted that other factors not varied systematically in this study, like temperature, stirring time, and their interaction effects, will also affect the average diameter of the particles, as mentioned previously elsewhere 28 .…”

Section: Resultsmentioning

confidence: 81%

Parameters influencing the size of chitosan-TPP nano- and microparticles

Sreekumar

Goycoolea

Moerschbacher

et al. 2018

Sci Rep

234

137

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Chitosan nanoparticles, produced by ionic gelation, are among the most intensely studied nanosystems for drug delivery. However, a lack of inter-laboratory reproducibility and a poor physicochemical understanding of the process of particle formation have been slowing their potential market applications. To address these shortcomings, the current study presents a systematic analysis of the main polymer factors affecting the nanoparticle formation driven by an initial screening using systematic statistical Design of Experiments (DoE). In summary, we found that for a given chitosan to TPP molar ratio, the average hydrodynamic diameter of the particles formed is strongly dependent on the initial chitosan concentration. The degree of acetylation of the chitosan was found to be the second most important factor involved in the system’s ability to form particles. Interestingly, viscosimetry studies indicated that the particle formation and the average hydrodynamic diameter of the particles formed were highly dependent on the presence or absence of salts in the medium. In conclusion, we found that by controlling two simple factors of the polymer solution, namely its initial concentration and its solvent environment, it is feasible to control in a reproducible manner the production and characteristics of chitosan particles ranging in size from nano- to micrometres.

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Section: Resultsmentioning

confidence: 81%

Parameters influencing the size of chitosan-TPP nano- and microparticles

Sreekumar

Goycoolea

Moerschbacher

et al. 2018

Sci Rep

234

137

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“…This method offers a simple and rapid preparation technique in the aqueous environment and is based on the electrostatic interaction of the amine groups of CS and the negatively charged groups of a polyanion such as tripolyphosphate (TPP). The size and surface characteristics of particles can be easily adjusted by varying the ratio between the polymer and polyanion .…”

Section: Polysaccharide Nanoparticlesmentioning

confidence: 99%

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Salatin

Khosroushahi

2017

J Cellular Molecular Medi

236

143

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Nanoparticulate drug/gene carriers have gained much attention in the past decades because of their versatile and tunable properties. However, efficacy of the therapeutic agents can be further enhanced using naturally occurring materials‐based nanoparticles. Polysaccharides are an emerging class of biopolymers; therefore, they are generally considered to be safe, non‐toxic, biocompatible and biodegradable. Considering that the target of nanoparticle‐based therapeutic strategies is localization of biomedical agents in subcellular compartments, a detailed understanding of the cellular mechanism involved in the uptake of polysaccharide‐based nanoparticles is essential for safe and efficient therapeutic applications. Uptake of the nanoparticles by the cellular systems occurs with a process known as endocytosis and is influenced by the physicochemical characteristics of nanoparticles such as size, shape and surface chemistry as well as the employed experimental conditions. In this study, we highlight the main endocytosis mechanisms responsible for the cellular uptake of polysaccharide nanoparticles containing drug/gene.

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“…In regards to particle size, nanoscale chitosan particles exhibit remarkable properties in its application such as high adsorption capacity [6], increase cellular uptake and drug release [11] and alleviate toxicological effect [12]. Previously, chitosan nanoparticles were synthesized using various methods, namely selfassembly [11,13], microemulsion [14], ultrafine milling [12] and ionotropic gelation [2,15,16]. The latter has been known as a simpler technique used for natural polymer nanoparticles preparation compared to others that require multi-experimental steps and use many harmful chemicals to assist preparation processes [11,17], or are hard to control the chitosan particle size [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Size-controlled chitosan nanoparticles prepared using ionotropic gelation

Budi¹,

Suliasih²,

Rahmawatia³

et al. 2020

ScienceAsia

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This work aimed at controlling the size of chitosan nanoparticles that were prepared by the ionotropic gelation method. Quantities of chitosan and sodium tripolyphosphate (STPP) were optimized to obtain a fine size and narrow size distribution of the nanoparticles. X-ray diffraction and selected area electron diffraction patterns indicated the existence of both crystalline and amorphous phases of chitosan. Particle size and size distribution were determined using transmission electron microscopy and dynamic laser scattering techniques. The size of the chitosan particles decreased with a reduction in STPP content due to a decrease in cross-link bonding and the formation of aggregate particles. Using this facile approach, the finest particles of chitosan were obtained in a range of 3-6 nm.

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Chitosan nanoparticles synthesis caught in action using microdroplet reactions

Cited by 51 publications

References 11 publications

Parameters influencing the size of chitosan-TPP nano- and microparticles

Parameters influencing the size of chitosan-TPP nano- and microparticles

Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles

Size-controlled chitosan nanoparticles prepared using ionotropic gelation

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