Chitosan Nanoparticles of Gamma-Oryzanol: Formulation, Optimization, and In vivo Evaluation of Anti-hyperlipidemic Activity

Abstract

The elevated blood levels of cholesterol and low-density lipoproteins result in hyperlipidemia. The available expensive prophylactic treatments are kindred with severe side effects. Therefore, we fabricated the polymeric nanoparticles of gamma-oryzanol to achieving the improved efficacy of drug. The nanoparticles were prepared by ionic gelation method and optimized using 2 full factorial design taking drug/polymer ratio (X), polymer/cross linking agent ratio (X), and stirring speed (X) as independent variables. The average particle size, percentage entrapment efficiency, and in vitro drug release at 2, 12, and 24 h were selected as response parameters. The factorial batches were statistically analyzed and optimized. The optimized nanoparticles were characterized with respect to particle size (141 nm) and zeta potential (+ 6.45 mV). Results obtained with the prepared and characterized formulation showed 83% mucoadhesion towards the intestinal mucosa. The in vitro findings were complemented well by in vivo anti-hyperlipidemic activity of developed formulation carried out in Swiss albino mouse model. The in vivo studies showed improved atherogenic index, malondialdehyde, and superoxide dismutase levels in poloxamer-407-induced hyperlipidemic animals when treated with oryzanol and gamma-oryzanol nanoformulation. Based on our findings, we believe that chitosan-mediated delivery of gamma-oryzanol nanoparticles might prove better in terms of anti-hyperlipidemic therapeutics.

Preparation of Chitosan Embedded Recombinant Human Epidermal Growth Factor Nanoparticles as Accelerating Compounds for Skin Remodeling in Chronic Lesions

“…Moreover, preserving the typical shape of polyhedrons as a result of using the NPs is another benefit of TPP (24,25,39). TEM and DLS were performed to determine the size range of NPs and the obtained results reported the size of NPs around 150±50nm, as confirmed in past studies on drug delivery uses (40). It is notable that, as discussed above, EGF has a positive charge while it is coated with the negative charges of chitosan.…”

Effects of curcumin and γ‑oryzanol solid dispersion on the brain of middle‑aged rats

“…Curcumin and γ-oryzanol compounds exhibit a wide range of pharmacological properties, including antioxidant, anti-inflammatory, anti-tumor, and anti-diabetic properties (9)(10)(11)(12)30). Nevertheless, both compounds have been reported to be limited with regard to their use as a nutritional product given their low water-solubility, resulting in low absorption and bioavailability (13)(14)(15)(16). The present study prepared curcumin and γ-oryzanol as a solid dispersion to enhance their water solubility.…”

“…Moreover, these two elements have been identified as compounds that increase antioxidant levels such as those of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) (9)(10)(11)(12). However, studies have found that the beneficial effects of curcumin and γ-oryzanol are limited by their low water-solubility, leading to low bioavailability (13)(14)(15)(16). Therefore, curcumin and γ-oryzanol were prepared in a solid dispersion to enhance their water solubility.…”

Process of Orlistat-Loaded Chitosan Nanoparticles Using Box–behnken Design – An Evaluation Study

“…FTIR spectra of orlistat, chitosan, TPP and their physical mixture were studied by KBr pellet method. Each spectrum of the sample was collected from 32 single average scans at a resolution of 4cm -1 in the absorption region of 400-4000cm -1 [15].…”