Chitosan Nanoparticles for Melanoma Cancer Treatment by Photodynamic Therapy and Electrochemotherapy Using Aminolevulinic Acid Derivatives

Ferreira, DM; Yy, Saga; Aluicio‐Sarduy, Eduardo; Tedesco, Antônio Cláudio

doi:10.2174/0929867311320140007

Cited by 28 publications

(13 citation statements)

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“…[11][12][13] Both of these polymers are widely reported for development of various drug delivery systems, in particular chitosan nanoparticles for melanoma treatment. 14,15 Natural compounds with antitumor properties are of great interest, since they are rather cheap and non-toxic for normal cells. Thymoquinone which was selected here as a model lipophilic drug, is a main active component of black seed essential oil.…”

Section: Introductionmentioning

confidence: 99%

Ultrasonically Assisted Polysaccharide Microcontainers for Delivery of Lipophilic Antitumor Drugs: Preparation and in Vitro Evaluation

Akasov

Bоrodinа

Zaytseva

et al. 2015

ACS Appl. Mater. Interfaces

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High toxicity, poor selectivity, and severe side effects are major drawbacks of anticancer drugs. Various drug delivery systems could be proposed to overcome these limitations. The aim of this study was to fabricate polysaccharide microcontainers (MCs) loaded with thymoquinone (TQ) by a one-step ultrasonication technique and to study their cellular uptake and cytotoxicity in vitro. Two MC fractions with a mean size of 500 nm (MC-0.5) and 2 μM (MC-2) were prepared and characterized. Uptake of the MCs by mouse melanoma M-3 cells was evaluated in both 2D (monolayer culture) and 3D (multicellular tumor spheroids) models by confocal microscopy, flow cytometry, and fluorimetry. The higher cytotoxicity of the TQ-MC-0.5 sample than the TQ-MC-2 fraction was in good correlation with higher MC-0.5 accumulation in the cells. The MC-0.5 beads were more promising than the MC-2 particles because of a higher cellular uptake in both 2D and 3D models, an enhanced antitumor effect, and a lower nonspecific toxicity.

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Section: Introductionmentioning

confidence: 99%

Ultrasonically Assisted Polysaccharide Microcontainers for Delivery of Lipophilic Antitumor Drugs: Preparation and in Vitro Evaluation

Akasov

Bоrodinа

Zaytseva

et al. 2015

ACS Appl. Mater. Interfaces

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“…The photosensitizer excited by ALA could contribute to the generation of reactive oxygen species, which induces oxidative damage and consequently leads to cell death [35]. Ferreira et al indicated that melanoma cancer could be treated by photodynamic therapy using ALA [36]. Prior studies observed that 5-ALA was a promising candidate for CRC diagnosis, and 5-ALA mediated photodynamic therapy could be a therapeutic strategy for CRC [37,38], which is consistent with our nding that ALA is an effective drug for CRC treatment.…”

Section: Establishment Of the Read Stage Prognostic Modelmentioning

confidence: 99%

Construction of a Prognostic Model for Predicting Overall Survival of Patients with Colorectal Cancer

Han¹,

Ding²

2020

Preprint

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Background: Colorectal cancer (CRC) is one of the most common malignancies. The purpose of this study is to construct a prognostic model for predicting the overall survival (OS) in patients with CRC. Methods: The mRNA-seq and miRNA-seq data of colon adenocarcinoma (COAD) and rectal adenocarcinoma (READ) were downloaded from The Cancer Genome Atlas (TCGA) database. The differentially expressed RNAs (DE-RNAs) between tumor and normal tissues were screened. The Kaplan-Meier and univariate Cox regression analysis were used to screen the survival-related genes. Functional enrichment analysis of survival-related genes was conducted, followed by protein-protein interaction (PPI) analysis. Subsequently, the potential drugs targeting differentially expressed mRNAs (DE-mRNAs) were investigated. Multivariate Cox regression analysis was then conducted to screen the independent prognostic factors, and these genes were used to establish a prognostic model. A receiver operator characteristic (ROC) curve was constructed, and the area under the curve (AUC) value of ROC was calculated to evaluate the specificity and sensitivity of the model. Results: A total of 855 survival-related genes were screened. These genes were mainly enriched in Gene Ontology (GO) terms, such as methylation, synapse organization, and methyltransferase activity; and pathway analysis showed that these genes were significantly involved in N-Glycan biosynthesis and the calcium signaling pathway. PPI analysis showed that aminolevulinate dehydratase (ALAD) and cholinergic receptor muscarinic 2 (CHRM2) served vital roles in the development of CRC. Aminolevulinic acid, levulinic acid, and loxapine might be potential drugs for CRC treatment. The prognostic models were built and the patients were divided into high-risk and low-risk groups based on the median of risk score (RS) as screening threshold. The OS for patients in the high-risk group was markedly shorter than that for patients in the low-risk group. Meanwhile, kazal type serine peptidase inhibitor domain 1 (KAZALD1), hippocalcin like 4 (HPCAL4), cadherin 8 (CDH8), synaptopodin 2 (SYNPO2), cyclin D3 (CCND3), and hsa_mir_26b may be independent prognostic factors that could be considered as therapeutic targets for CRC.Conclusion: We established prognostic models that could predict the OS for CRC patients and may assist clinicians in providing personalized and precision treatment in this patient population.Highlights:1. ALAD served a vital role in the development of CRC.2. CHRM2 played a role in CRC development by affecting the calcium signaling pathway.3. Aminolevulinic acid, levulinic acid, and loxapine might be potential drugs for treating CRC.4. KAZALD1 and HPCAL4 were associated with the OS of CRC.5. CDH8, SYNPO2, CCND3, and hsa-mir-26b were closely related to the prognostic of CRC staging.

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“…Moreover, the use of multimodal therapies leading to synergistic effect has become a promising approach to enhance anti-cancer therapy, reduce undesirable systemic toxicity and side effects (123). PDT along with magnetohyperthermia, photothermal therapy and electrochemotherapy using nanomaterials has also been studied with the perspective of achieving enhanced therapeutic cancer treatments (123–130). Magnetohyperthermia refers to a temperature rise caused by energy dissipation in the form of heat from magnetic nanoparticles that respond to an alternated frequency magnetic field.…”

Section: Perspectives On the Application Of Nanotechnology And Pdt Fomentioning

confidence: 99%

Perspectives on the application of nanotechnology in photodynamic therapy for the treatment of melanoma

2014

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Malignant melanoma is the most aggressive form of skin cancer and has been traditionally considered difficult to treat. The worldwide incidence of melanoma has been increasing faster than any other type of cancer. Early detection, surgery, and adjuvant therapy enable improved outcomes; nonetheless, the prognosis of metastatic melanoma remains poor. Several therapies have been investigated for the treatment of melanoma; however, current treatment options for patients with metastatic disease are limited and non-curative in the majority of cases. Photodynamic therapy (PDT) has been proposed as a promising minimally invasive therapeutic procedure that employs three essential elements to induce cell death: a photosensitizer, light of a specific wavelength, and molecular oxygen. However, classical PDT has shown some drawbacks that limit its clinical application. In view of this, the use of nanotechnology has been considered since it provides many tools that can be applied to PDT to circumvent these limitations and bring new perspectives for the application of this therapy for different types of diseases. On that ground, this review focuses on the potential use of developing nanotechnologies able to bring significant benefits for anticancer PDT, aiming to reach higher efficacy and safety for patients with malignant melanoma.

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Chitosan Nanoparticles for Melanoma Cancer Treatment by Photodynamic Therapy and Electrochemotherapy Using Aminolevulinic Acid Derivatives

Cited by 28 publications

References 0 publications

Ultrasonically Assisted Polysaccharide Microcontainers for Delivery of Lipophilic Antitumor Drugs: Preparation and in Vitro Evaluation

Ultrasonically Assisted Polysaccharide Microcontainers for Delivery of Lipophilic Antitumor Drugs: Preparation and in Vitro Evaluation

Construction of a Prognostic Model for Predicting Overall Survival of Patients with Colorectal Cancer

Perspectives on the application of nanotechnology in photodynamic therapy for the treatment of melanoma

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