Chitosan nanocapsules: Effect of chitosan molecular weight and acetylation degree on electrokinetic behaviour and colloidal stability

Santander-Ortega, Manuel J.; Peula-García, José Manuel; Goycoolea, Francisco M.; Ortega-Vinuesa, J.L.

doi:10.1016/j.colsurfb.2010.10.019

Cited by 79 publications

(58 citation statements)

References 42 publications

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“…This will determine the hydrophilicity of the complexes and therefore, the amount of counter ions needed for repulsive forces. It is known that chitosans with higher DA enhance the hydrophilicity of the surface and stability, even at low ionic concentration (Santander-Ortega et al, 2011). Hence, the relative high DA of the chitosan sample used in the present studies (DA=30%) may explain the stability of the complexes.…”

Section: Discussionmentioning

confidence: 82%

“…As it was described in our materials section, the Opti-MEM has moderate saline content. The presence of specific ions might allow relative stabilization of the complexes due to hydration forces as found in previous studies on chitosan-based nanocapsules (Goycoolea et al, 2012;Santander-Ortega et al, 2011)(ref. Santander-Ortega et al 2011, Goycoolea et al 2011.…”

Section: Discussionmentioning

confidence: 82%

“…The presence of specific ions might allow relative stabilization of the complexes due to hydration forces as found in previous studies on chitosan-based nanocapsules (Goycoolea et al, 2012;Santander-Ortega et al, 2011)(ref. Santander-Ortega et al 2011, Goycoolea et al 2011. It is claimed that ions from the medium could accumulate in the proximity of the hydrophilic surface sites of the complexes.…”

Section: Discussionmentioning

confidence: 82%

“…It is claimed that ions from the medium could accumulate in the proximity of the hydrophilic surface sites of the complexes. Subsequently, this effect will produce short-range repulsive hydration forces, which would lead the stabilization of the complexes (López-León et al, 2008;Santander-Ortega et al, 2011). The extension of this hydration forces is highly dependent on the DA of the chitosan used and on the type of ion.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Chitosan as a non-viral co-transfection system in a cystic fibrosis cell line

Fernández

Santos-Carballal

Weber

et al. 2016

International Journal of Pharmaceutics

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Successful gene therapy requires the development of suitable vehicles for the selective and efficient delivery of genes to specific target cells at the expense of minimal toxicity. In this work, we investigated a non-viral gene delivery system based on chitosan (CS) to specifically address cystic fibrosis (CF). Thus, electrostatic self-assembled CS-pEGFP and CS-pEGFPsiRNA complexes were prepared from high-pure fully characterized CS (Mw ~20 kDa and degree of acetylation ~30%). The average diameter of positively-charged complexes (i.e.  ~ +25 mV) was ~200 nm. The complexes were found relatively stable over 14 h in OptiMEM. Cell viability study did not show any significant cytotoxic effect of the CS-based complexes in a human bronchial cystic fibrosis cell line (s). We evaluated the transfection efficiency of this cell line with both CS-pEGFP and co-transfected with CS-pEGFP-siRNA complexes at (N/P) charge ratio of 12. We reported an increase in the fluorescence intensity of CS-pEGFP and a reduction in the cells co-transfected with CS-pEGFP-siRNA. This study shows proof-of-principle that co-transfection with chitosan might be an effective delivery system in a human CF cell line. It also offers a potential alternative to further develop therapeutic strategies for inherited disease treatments, such as CF.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Chitosan as a non-viral co-transfection system in a cystic fibrosis cell line

Fernández

Santos-Carballal

Weber

et al. 2016

International Journal of Pharmaceutics

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“…This study shows that the ζ-potential of the droplets covered by chitosan does not depend on the molecular characteristics of the polyelectrolyte and decreases with increasing pH of the dispersion. Similar electrokinetic and stability behavior of oilcore nanocapsules coated by chitosan derivatives at different pH and salt concentration have also been presented in our previous study (11). Recently, we have investigated the influence electrical properties of chitosan on the formation of polyelectrolyte multilayer films on oxide nanoparticles (12)(13)(14).…”

Section: Introductionmentioning

confidence: 74%

Electrokinetic behavoir of chitosan adsorbed on o/w nanoemulsion droplets

Goycoolea

Milkova

2017

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Please cite this article as: Francisco M.Goycoolea, Viktoria Milkova, Electrokinetic behavoir of chitosan adsorbed on o/w nanoemulsion droplets, Colloids and Surfaces A: Physicochemical and Engineering Aspects http://dx.Highlights  Stability of O/W nanoemulsion in the presence of chitosan;  Fraction of condensed counterions released from chitosan molecules upon adsorption on weak charged surface;  Electrical polarizability of chitosan molecule with condensed counterions. AbstractChitosans with different molecular weight and degree of acetylation (DA) were adsorbed onto lecithin stabilized nanoemulsion droplets at pH4.7. The electrokinetic properties of the nanoemulsion were investigated by using dynamic light scattering. According to the experimental data, the isoelectric point (IEP) of the droplets in the suspension at the presence of oppositely charged chitosan, does not depend on the polyelectrolyte molecular weight (when DA of chitosan is lower than 32 %). Due to the high charge density of these polymer chains and low surface charge density of the lecithin stabilized droplets, the chitosan molecules retain part of the condensed counterions upon the adsorption process. Thus, the registered electrophoretic mobility corresponding to the "effective", and not "real", the electrokinetic charge of the droplets covered by a polymer layer. The stability behavior of the nanoemulsion in the presence of chitosan with lower charge density is different, and the IEP and stabilization of the system is achieved at higher polymer concentration. The coincidence in the polyelectrolyte concentration corresponding to IEP and minimum stability of the nanoemulsion against coagulation agrees with the notion that the electrostatic interactions are predominant in the coating layer formation. In spite of the similar electrokinetic behavior of the droplets covered with chitosan with low DA and different molecular weight, the polymers have different biological activity which might be results from different charge density of the molecules. In the present study, we show that the electrical polarizability is a complex parameter that can allow to more detailed characterization of the electrical properties of the chitosan.

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Effect of molecular weight of chitosan on quercetin‐loaded chitosan nanoparticles

Geng,

Zhang,

Sun

et al. 2024

J Sci Food Agric

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BACKGROUNDThe widespread use of quercetin is limited by its instability, low solubility and poor oral bioavailability. Encapsulation of quercetin using a nanoparticle delivery system is an effective way to overcome these drawbacks.RESULTSThe effect of the molecular weight (Mw) of chitosan (CS) (100, 200, 500 and 1000 kDa) on quercetin‐loaded chitosan nanoparticles (QCNPs) was investigated. The structure, stability, release properties and antioxidant activities of the nanoparticles (QCNP‐10, QCNP‐20, QCNP‐50 and QCNP‐100) were assessed. Particle size of QCNPs decreased and polydispersity index increased with the increasing Mw of CS. The main forces involved in the formation of QCNPs were hydrogen bonding and hydrophobic interaction. X‐ray diffraction verified that quercetin was loaded into CS nanoparticles. The photostability and thermal stability of QCNPs increased with increasing Mw of CS. QCNP‐100 exhibited the lowest release rate in a mixture of water and anhydrous ethanol. The antioxidant activities of QCNPs were enhanced with increasing Mw of CS, and QCNP‐100 possessed the highest antioxidant activities, which might be relevant to its smallest particle size.CONCLUSIONOverall, these results revealed that the Mw of CS affected the properties of QCNPs, and QCNP‐100 possessed the smallest particle, best stability, lowest release rate and highest antioxidant activities. © 2024 Society of Chemical Industry.

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Chitosan nanocapsules: Effect of chitosan molecular weight and acetylation degree on electrokinetic behaviour and colloidal stability

Cited by 79 publications

References 42 publications

Chitosan as a non-viral co-transfection system in a cystic fibrosis cell line

Chitosan as a non-viral co-transfection system in a cystic fibrosis cell line

Electrokinetic behavoir of chitosan adsorbed on o/w nanoemulsion droplets

Effect of molecular weight of chitosan on quercetin‐loaded chitosan nanoparticles

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