Chitosan microparticles containing plasmid DNA as potential oral gene delivery system

Guliyeva, Ulker; Öner, Filiz; Özsoy, Şule Yurdagül; Haziroğlu, Rıfkı

doi:10.1016/j.ejpb.2005.08.006

Cited by 126 publications

(59 citation statements)

References 31 publications

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“…The influence of the pH of the release medium on the in vitro release of pDNA from chitosan microparticles was also investigated [80]. It was observed that the release of pDNA from chitosan microparticles in simulated gastric (pH 2.1), simulated intestinal (pH 6.47) and acidic PBS (pH 4.5) medium was pH-dependent: as the pH of the release medium increased, the release profile decreased.…”

Section: Influence Of Phmentioning

confidence: 95%

Biomedical Activity of Chitin/Chitosan Based Materials—Influence of Physicochemical Properties Apart from Molecular Weight and Degree of N-Acetylation

et al. 2011

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Abstract:The physicochemical nature of chitin and chitosan, which influences the biomedical activity of these compounds, is strongly related to the source of chitin and the conditions of the chitin/chitosan production process. Apart from widely described key factors such as weight-averaged molecular weight (M W ) and degree of N-acetylation (DA), other physicochemical parameters like polydispersity (M W /M N ), crystallinity or the pattern of acetylation (P A ) have to be taken into consideration. From the biological point of view, these parameters affect a very important factor-the solubility of chitin and chitosan in water and organic solvents. The physicochemical properties of chitosan solutions can be controlled by manipulating solution conditions (temperature, pH, ionic strength, concentration, solvent). The degree of substitution of the hydroxyl and the amino groups or the degree of quaternization of the amino groups also influence the mechanical and biological properties of chitosan samples. Finally, a considerable research effort has been directed towards developing safe and efficient chitin/chitosan-based products because many factors, like the size of nanoparticles, can determine the biomedical characteristics of medicinal products. The influence of these factors on the biomedical activity of chitin/chitosan-based products is presented in this report in more detail. OPEN ACCESSPolymers 2011, 3 1876

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Section: Influence Of Phmentioning

confidence: 95%

Biomedical Activity of Chitin/Chitosan Based Materials—Influence of Physicochemical Properties Apart from Molecular Weight and Degree of N-Acetylation

et al. 2011

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“…The oral delivery system of a DNA vaccine must overcome the harsh environment and barriers in the gastrointestinal (GI) tract. Recently, many studies have shown that oral delivery systems in the form of nanoparticulates have the potential to increase the stability and absorption of protein and plasmid DNA via the GI tract (7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of Chitosan-Coated Liposomes for Oral DNA Vaccine: The Improvement of Peyer’s Patch Targeting Using a Polyplex-Loaded Liposomes

et al. 2010

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Abstract. The aim of this study was to develop chitosan-coated and polyplex-loaded liposomes (PLLs) containing DNA vaccine for Peyer's patch targeting. Plain liposomes carrying plasmid pRc/CMV-HBs were prepared by the reverse-phase evaporation method. Chitosan coating was carried out by incubation of the liposomal suspensions with chitosan solution. Main lipid components of liposomes were phosphatidylcholine/cholesterol. Sodium deoxycholate and dicetyl phosphate were used as negative charge inducers. The zeta potentials of plain liposomes were strongly affected by the pH of the medium. Coating with chitosan variably increased the surface charges of the liposomes. To increase the zeta potential and stability of the liposome, chitosan was also used as a DNA condensing agent to form a polyplex. The PLLs were coated with chitosan solution. In vivo study of PLLs was carried out in comparison with chitosan-coated liposomes using plasmid encoding green fluorescence protein as a reporter. A single dose of plasmid equal to 100 μg was intragastrically inoculated into BALB/c mice. The expression of green fluorescence protein (GFP) was detected after 24 h using a confocal laser scanning microscope. The signal of GFP was obtained from positively charged chitosan-coated liposomes but found only at the upper part of duodenum. With chitosan-coated PLL540, the signal of GFP was found throughout the intestine. Chitosan-coated PLL demonstrated a higher potential to deliver the DNA to the distal intestine than the chitosan-coated liposomes due to the increase in permanent positive surface charges and the decreased enzymatic degradation.

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“…To overcome these obstacles, microparticles and nanoparticles are a promising approach. Chitosan-DNA microparticles could protect the encapsulated plasmid DNA from nuclease degradation [49]. In in vivo-animal studies, a blue color was observed with X-gal staining of histological stomach and small intestine sections after oral administration of chitosan-DNA microparticles.…”

Section: Oral Routementioning

confidence: 94%

Gene Therapy for Gastric Diseases

Fumoto¹,

Nishi²,

Nakamura³

et al. 2008

CGT

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Gene therapy for gastric cancer and gastric ulcer is a rationalized strategy since various genes correlate with these diseases. Since gene expressions in non-target tissues/cells cause side effects, a selective gene delivery system targeted to the stomach and/or cancer must be developed.The route of vector transfer (direct injection, systemic, intraperitoneal, gastric serosal surface and oral administration) is an important issue which can determine efficacy and safety. Strategies for cancer gene therapy can be categorized as suicide gene therapy, growth inhibition and apoptosis induction, immunotherapy, anti-angiogenesis, and others. Combination of the target gene with other genes and/or strategies such as chemotherapy and virotherapy is promising. Candidates for treatment of gastric ulcer are vascular endothelial growth factor, angiopoietin-1, serum response factor, and cationic host defense peptide cathelicidin. In this review, we discuss stomach-and cancer-targeted gene transfer methods and summarize gene therapy trials for gastric cancer and gastric ulcer.

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Chitosan microparticles containing plasmid DNA as potential oral gene delivery system

Cited by 126 publications

References 31 publications

Biomedical Activity of Chitin/Chitosan Based Materials—Influence of Physicochemical Properties Apart from Molecular Weight and Degree of N-Acetylation

Biomedical Activity of Chitin/Chitosan Based Materials—Influence of Physicochemical Properties Apart from Molecular Weight and Degree of N-Acetylation

Development and Evaluation of Chitosan-Coated Liposomes for Oral DNA Vaccine: The Improvement of Peyer’s Patch Targeting Using a Polyplex-Loaded Liposomes

Gene Therapy for Gastric Diseases

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