Chitosan mediated enhancement of hydrolysable tannin in Phyllanthus debilis Klein ex Willd via plant cell suspension culture

Malayaman, V; Sisubalan, N; Senthilkumar, R.; Mohamed, S Sheik; Ranjithkumar, R.; Basha, M. Ghouse

doi:10.1016/j.ijbiomac.2017.03.138

Cited by 25 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…cell suspension cultures 29 , hydrolysable tannin in Phyllanthus debilis Klein ex Willd. cell suspension cultures 30 , plumbagin in Plumbago indica L. adventitious root cultures 31 , and withanolides in Withania somnifera (L.) Dunal adventitious root cultures 32 . Whereas, chitosan elicitation conditions required for the maximal enhancement of the abovementioned phytochemicals are inconsistent.…”

Section: Discussionmentioning

confidence: 99%

Chitosan promoting formononetin and calycosin accumulation in Astragalus membranaceus hairy root cultures via mitogen-activated protein kinase signaling cascades

Gai

Jiao

Wang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Chitosan, behaving as a potent biotic elicitor, can induce plant defense response with the consequent enhancement in phytoalexin accumulation. Accordingly, chitosan elicitation was conducted to promote the production of two phytoalexins, i . e . formononetin and calycosin (also known as health-promoting isoflavones), in Astragalus membranaceus hairy root cultures (AMHRCs). Compared with control, 12.45- and 6.17-fold increases in the yields of formononetin (764.19 ± 50.81 μg/g DW) and calycosin (611.53 ± 42.22 μg/g DW) were obtained in 34 day-old AMHRCs treated by 100 mg/L of chitosan for 24 h, respectively. Moreover, chitosan elicitation could cause oxidative burst that would induce the expression of genes ( MPK3 and MPK6 ) related to mitogen-activated protein kinase signaling (MAPK) cascades, which contributed to the transcriptional activation of pathogenesis-related genes ( β-1 , 3-glucanase , Chitinase , and PR-1 ) and eight biosynthesis genes involved in the calycosin and formononetin pathway. Overall, the findings in this work not only highlight a feasible chitosan elicitation practice to enhance the in vitro production of two bioactive isoflavones for nutraceutical and food applications, but also contribute to understanding the phytoalexin biosynthesis in response to chitosan elicitation.

show abstract

Section: Discussionmentioning

confidence: 99%

Chitosan promoting formononetin and calycosin accumulation in Astragalus membranaceus hairy root cultures via mitogen-activated protein kinase signaling cascades

Gai

Jiao

Wang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…A cultura de calo de Fagonia indica suplementada com quitosana exibiu níveis mais altos de compostos fenólicos e flavonoides, e atividade antioxidantes [12]. A adição de 150 mg de quitosana, em 100 mL de meio líquido MS, aumentou o nível de tanino hidrolisável em suspensão de células de Phyllanthus debilis na fase estacionária [13]. A produção de artemisinina de Artemisia annua em cultura de raízes aumentou seis vezes com a adição de quitosana [10].…”

Section: Efeito Da Quitosana Nos Compostos Voláteisunclassified

“…A quitosana apresentou potencial para aumentar o acúmulo de biomassa e polifenóis em culturas de calos de Fagonia indica [12]. Malayaman et al (2017) [13] relataram o uso de quitosana para aumentar a produção de tanino hidrolisável usando a cultura de suspensão celular em P. debilis.…”

Section: Introductionunclassified

Elicitação com quitosana no crescimento e nos compostos voláteis de Mentha arvensis in vitro

et al. 2020

View full text Add to dashboard Cite

Elicitores são substâncias com a capacidade de induzir a produção de metabólitos secundários específicos. Objetivou-se avaliar o efeito de quitosana suplementada no meio de cultura no crescimento, produção de pigmentos fotossintéticos e compostos voláteis de Mentha arvensis. Segmentos nodais de M. arvensis foram inoculados em meio MS suplementados com cinco concentrações de quitosana (0, 50, 100, 150 e 200 mg L-1). Após 30 dias de cultivo, avaliou-se o comprimento de brotos, as biomassas secas de folha, caule, raiz e total. Além disso, as análises dos compostos voláteis e pigmentos fotossintéticos foram investigados. A adição de quitosana em 100 mg L-1 promoveu maiores acúmulos de biomassas secas em todos os órgãos avaliados. Por outro lado, maiores concentrações de quitosana (150 e 200 mg L-1) apresentaram efeito fitotóxico nas plântulas. Maiores teores de clorofila a e carotenoides foram observados sem a suplementação com quitosana e clorofila b com 200 mg L-1. A quitosana influenciou a concentração dos constituintes voláteis in vitro. Na menor concentração de quitosana (50 mg L-1) houve maior acúmulo de pulegona, 100 mg L-1 de limoneno e 200 mg L-1 de mentol e mentona.

show abstract

Role of Chitosan Nanoparticles in Regulation of Plant Physiology Under Abiotic Stress

Arif

Siddiqui

Hayat

2021

Sustainable Agriculture Reviews 53

View full text Add to dashboard Cite

Chitosan mediated enhancement of hydrolysable tannin in Phyllanthus debilis Klein ex Willd via plant cell suspension culture

Cited by 25 publications

References 39 publications

Chitosan promoting formononetin and calycosin accumulation in Astragalus membranaceus hairy root cultures via mitogen-activated protein kinase signaling cascades

Chitosan promoting formononetin and calycosin accumulation in Astragalus membranaceus hairy root cultures via mitogen-activated protein kinase signaling cascades

Elicitação com quitosana no crescimento e nos compostos voláteis de Mentha arvensis in vitro

Role of Chitosan Nanoparticles in Regulation of Plant Physiology Under Abiotic Stress

Contact Info

Product

Resources

About