Chitosan–hyaluronic acid nanoparticles loaded with heparin for the treatment of asthma

Oyarzún-Ampuero, Felipe; Brea, José; Loza, Marı́a Isabel; Torres, Dolores; Alonso, Marı́a José

doi:10.1016/j.ijpharm.2009.04.009

Cited by 193 publications

(111 citation statements)

References 27 publications

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“…As can be observed in Figure 2, pullulan-based nanoparticles evidence similar structure and morphology, corresponding to spherical and compact nanoparticles. This morphology corresponds to that widely reported for many formulations of polysaccharide-based nanoparticles (Calvo et al, 1997b;Goycoolea et al, 2009;Oyarzun-Ampuero et al, 2009;Sarmento et al, 2006;TeijeiroOsorio et al, 2009;Zorzi et al, 2011).…”

Section: Preparation and Characterisation Of Nanoparticlessupporting

confidence: 89%

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Dionísio

Cordeiro

Remuñán‐López

et al. 2013

European Journal of Pharmaceutical Sciences

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Polymeric nanoparticles have revealed very effective in transmucosal delivery of proteins. Polysaccharides are among the most used materials for the production of these carriers, owing to their structural flexibility and propensity to evidence biocompatibility and biodegradability. In parallel, there is a preference for the use of mild methods for their production, in order to prevent protein degradation, ensure lower costs and easier procedures that enable scaling up.In this work we propose the production of pullulan-based nanoparticles by a mild method of polyelectrolyte complexation. As pullulan is a neutral polysaccharide, sulfated and aminated derivatives of the polymer were synthesized to provide pullulan with a charge. These derivatives were then complexed with chitosan and carrageenan, respectively, to produce the nanocarriers. Positively charged nanoparticles of 180-270 nm were obtained, evidencing ability to associate bovine serum albumin, which was selected as model protein. In PBS pH 7.4, pullulan-based nanoparticles were found to have a burst release of 30% of the protein, which maintained up to 24h. Nanoparticle size and zeta potential were preserved upon freeze-drying in the presence of appropriate cryoprotectants. A factorial design was approached to assess the cytotoxicity of raw materials and nanoparticles by the metabolic test MTT. Nanoparticles demonstrated to not cause overt toxicity in a respiratory cell model (Calu-3). Pullulan has, thus, demonstrated to hold potential for the production of nanoparticles with an application in protein delivery.

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Section: Preparation and Characterisation Of Nanoparticlessupporting

confidence: 89%

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Dionísio

Cordeiro

Remuñán‐López

et al. 2013

European Journal of Pharmaceutical Sciences

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“…The rapid development of nano-technology of pharmaceutics brings new hope to resolve the difficulties in the ocular delivery. Such technologies include liposomes (Alsarra et al, 2008;Shehata et al, 2008;Pukanud et al, 2009;Mehanna et al, 2010), polymer nanoparticles (Oyarzun-Ampuero et al, 2009), lipid nanoparticles (Gokce et al, 2008), microemulsions (Chan et al, 2007), etc. Such formulations are suitable to be applied to a topical instillation for treatment of local ophthalmic diseases, and meanwhile the systematic adverse effects and clinical inconvenience during the treatment can be minimized.…”

Section: Introductionmentioning

confidence: 99%

Low molecular weight chitosan-coated liposomes for ocular drug delivery:In vitroandin vivostudies

Li¹,

Zhuang²,

Wang³

et al. 2011

Drug Delivery

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“…The positive zeta-potential values indicate that the surface of the nanosystems is mostly composed of CS [26]. This net positive charge of the particle is desirable to promote electrostatic interaction with the ocular mucin [27].…”

Section: Particle Size and Zeta Potentialmentioning

confidence: 99%

Preparation and Characterization of Novel Drug-Inserted-Montmorillonite Chitosan Carriers for Ocular Drug Delivery

Hou¹,

Gui²,

Hu³

et al. 2015

ANP

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The objective of the study was to investigate the potential of montmorillonite as a sustained carrier in the preparation of drug-loaded nanoparticles for prolonged ocular application. Nanoparticles were prepared by ionic gelation of chitosan with sodium tripolyphosphate (TPP). Betaxolol hydrochloride (BH) was applied as a model drug. TG, FT-IR, TEM, DLS and XRD have been employed in the characterization of drug-encapsulated chitosan-montmorillonite/TPP nanoparticles (CS-MMT/TPP NPs). TEM images showed that the particles were spherical in shape and had a rough surface. The size range of the nanoparticles was between 338 and 585 nm with positive zeta potential values from 24 mV to 36 mV and encapsulation efficiency values ranging from 12.27% to 50.92%. In vitro sustained drug release was observed with the BH-loaded nanoparticles in artificial tears (pH 7.4). The results of FT-IR, TG and XRD showed that the drug was coated with CS-MMT/TPP NPs. In the mucoadhesion studies, an interaction was found between drug-loaded CS-MMT/TPP NPs and mucin, which could enhance precorneal residence time and hence facilitate an effective sustained release. The optimized formulation was determined to be non-irritant and tolerable by modified Draize test. Therefore, the BH-loading CS-MMT/TPP NPs developed are a promising carrier for controlled drug delivery to the eye.

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Chitosan–hyaluronic acid nanoparticles loaded with heparin for the treatment of asthma

Cited by 193 publications

References 27 publications

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Low molecular weight chitosan-coated liposomes for ocular drug delivery:In vitroandin vivostudies

Preparation and Characterization of Novel Drug-Inserted-Montmorillonite Chitosan Carriers for Ocular Drug Delivery

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