Chitosan/Hyaluronic acid/Alginate and an assorted polymers loaded with honey, plant, and marine compounds for progressive wound healing—Know-how

Prasathkumar, Murugan; Sadhasivam, S.

doi:10.1016/j.ijbiomac.2021.07.067

Cited by 128 publications

(101 citation statements)

References 272 publications

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“…These stages can be affected by factors such as infection or ischemia, which can slow the wound healing rate ( Das and BakerMills and Sr, 2014 ; Das and Baker, 2016 ). Therefore, in many studies, various materials, such as collagen, CS, gelatin and synthetic polymers, have been used to deliver antibiotics or growth factors to skin wounds to accelerate the healing rate of skin wounds ( Nurkesh et al, 2020 ; Kaiser et al, 2021 ; Prasathkumar and Sadhasivam, 2021 ). Some researchers developed CS scaffolds containing basic bFGF and treated the pressure ulcers of elderly mice, and results showed that CS/bFGF scaffolds significantly accelerated wound healing compared with pure CS group ( Park et al, 2009 ).…”

Section: Resultsmentioning

confidence: 99%

Acceleration of Healing in Full-Thickness Wound by Chitosan-Binding bFGF and Antimicrobial Peptide Modification Chitosan Membrane

Hou

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Skin wound healing is an important clinical challenge, and the main treatment points are accelerating epidermal regeneration and preventing infection. Therefore, it is necessary to develop a wound dressing that can simultaneously cure bacterial infections and accelerate wound healing. Here, we report a multifunctional composite wound dressing loaded with chitosan (CS)-binding bFGF (CSBD-bFGF) and antimicrobial peptides (P5S9K). First, CS was used as the dressing matrix material, and P5S9K was encapsulated in CS. Then, CSBD-bFGF was designed by combining recombinant DNA technology and tyrosinase treatment and modified on the dressing material surface. The results show that the binding ability of CSBD-bFGF and CS was significantly improved compared with that of commercial bFGF, and CSBD-bFGF could be controllably released from the CS dressing. More importantly, the prepared dressing material showed excellent antibacterial activity in vivo and in vitro and could effectively inhibit the growth of E. coli and S. aureus. Using NIH3T3 cells as cellular models, the results showed that the CSBD-bFGF@CS/P5S9K composite dressing was a friendly material for cell growth. After cells were seeded on the composite dressing surface, collagen-1 (COL-1) and vascular endothelial growth factor (VEGF) genes expression in cells were significantly upregulated. Finally, the full-thickness wound of the rat dorsal model was applied to analyse the tissue repair ability of the composite dressing. The results showed that the composite dressing containing CSBD-bFGF and P5S9K had the strongest ability to repair skin wounds. Therefore, the CSBD-bFGF@CS/P5S9K composite dressing has good antibacterial and accelerated wound healing abilities and has good application prospects in the treatment of skin wounds.

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Section: Resultsmentioning

confidence: 99%

Acceleration of Healing in Full-Thickness Wound by Chitosan-Binding bFGF and Antimicrobial Peptide Modification Chitosan Membrane

Hou

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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“…The antibacterial activity of honey and chitosan has been previously reported in the literature [ 8 , 21 , 44 ]. The antibacterial action of the hydrogel dressing can be explained by the synergistic effect of chitosan and honey.…”

Section: Resultsmentioning

confidence: 99%

“…Chitosan is a well-known natural biopolymer for wound healing applications. It depolymerizes into N-acetyl-β-D-glucosamine, which initiates the proliferation of fibroblasts and accelerates collagen synthesis and deposition [ 8 ]. Polyvinyl alcohol (PVA) is a synthetic polymer synthesized from the hydrolysis of acetate groups of polyvinyl acetate.…”

Section: Introductionmentioning

confidence: 99%

Physically Crosslinked Chitosan/PVA Hydrogels Containing Honey and Allantoin with Long-Term Biocompatibility for Skin Wound Repair: An In Vitro and In Vivo Study

Koosha

Aalipour

Shirazi

et al. 2021

JFB

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Chitosan/PVA hydrogel films crosslinked by the freeze–thaw method and containing honey and allantoin were prepared for application as wound dressing materials. The effects of the freeze–thaw process and the addition of honey and allantoin on the swelling, the gel content and the mechanical properties of the samples were evaluated. The physicochemical properties of the samples, with and without the freeze–thaw process, were compared using FTIR, DSC and XRD. The results showed that the freeze–thaw process can increase the crystallinity and thermal stability of chitosan/PVA films. The freeze–thaw process increased the gel content but did not have a significant effect on the tensile strength. The presence of honey reduced the swelling and the tensile strength of the hydrogels due to hydrogen bonding interactions with PVA and chitosan chains. Long-term cell culture experiments using normal human dermal fibroblast (NHDF) cells showed that the hydrogels maintained their biocompatibility, and the cells showed extended morphology on the surface of the hydrogels for more than 30 days. The presence of honey significantly increased the biocompatibility of the hydrogels. The release of allantoin from the hydrogel was studied and, according to the Korsmeyer–Peppas and Weibull models, the mechanism was mainly diffusional. The results for the antimicrobial activity against E. coli and S. aureus bacteria showed that the allantoin-containing samples had a more remarkable antibacterial activity against S. aureus. According to the wound healing experiments, 98% of the wound area treated by the chitosan/PVA/honey hydrogel was closed, compared to 89% for the control. The results of this study suggest that the freeze–thaw process is a non-toxic crosslinking method for the preparation of chitosan/PVA hydrogels with long term biocompatibility that can be applied for wound healing and skin tissue engineering.

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“…In this regard, electrospun nanofibers are one of the most effective wound dressing products because they have morphological similarities to skin ECM, such as a large specific surface area and a porous nature that promotes homeostasis, exudate absorption, gas permeability, cell adhesion, migration, and proliferation with the goal of improving healing. [163,[168][169][170] In addition, considerable improvement has been made in advancing clinical treatments for wound care over the past few decades. Wound dressings made of nanofiber polymers with antibacterial effects and skin regeneration capabilities are good choices for preventing wound infection and speeding wound healing.…”

Section: Polymer Advantages and Limitationsmentioning

confidence: 99%

Antimicrobial Synthetic and Natural Polymeric Nanofibers as Wound Dressing: A Review

et al. 2022

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Since ancient times, wound dressings have experienced many significant improvements. Evolution began using natural materials to merely cover wounds and advanced to used innovative techniques that can be customized to perform different impressive functions. Recent wound dressings, which are made of electrospun polymers, contain different active compounds, such as antimicrobial agents, that aid in wound healing and prevent dehydration and infection. The mentioned issues may influence the healing process, leading even to serious health risks for the patients. As a result, scientists are now working on novel wound bandages with improved antimicrobial properties. Electrospun polymeric nanofibers, because of their structural similarities to normal skin's extracellular matrix (ECM), bactericidal activity, and appropriateness to distribute bioactive molecules to the wound location, are regarded as good resources for enhancing skin regeneration and controlling wound infection. Herein, the latest findings on approaches for producing antimicrobial polymeric nanofibers using electrospinning and related processes are discussed. Recent advances in antibacterial biopolymeric nanofibers incorporating antimicrobial nanoparticles (silver, zinc oxide, copper oxide, etc.) are discussed. This review paper may raise significant issues, encourage additional research, and offer important insight into the potential area of antibacterial polymeric fibers.

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Chitosan/Hyaluronic acid/Alginate and an assorted polymers loaded with honey, plant, and marine compounds for progressive wound healing—Know-how

Cited by 128 publications

References 272 publications

Acceleration of Healing in Full-Thickness Wound by Chitosan-Binding bFGF and Antimicrobial Peptide Modification Chitosan Membrane

Acceleration of Healing in Full-Thickness Wound by Chitosan-Binding bFGF and Antimicrobial Peptide Modification Chitosan Membrane

Physically Crosslinked Chitosan/PVA Hydrogels Containing Honey and Allantoin with Long-Term Biocompatibility for Skin Wound Repair: An In Vitro and In Vivo Study

Antimicrobial Synthetic and Natural Polymeric Nanofibers as Wound Dressing: A Review

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