Chitosan-gelatin porous scaffold incorporated with Chitosan nanoparticles for growth factor delivery in tissue engineering

Azizian, Sara; Hadjizadeh, Afra; Niknejad, Hassan

doi:10.1016/j.carbpol.2018.07.023

Cited by 113 publications

(55 citation statements)

References 42 publications

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“…Natural ECM that is composed of a nanofibrous network containing biomolecules provides structural support for the proliferation, migration, and differentiation of cells . The design of biomaterials enables to encapsulate biomolecules effectively, release them significantly, and enhance scaffolds regenerative capacity . With this regard, some strategies have focused on the appropriate design and development of biomaterial scaffolds that are incorporated with biomolecules to regulate their release profiles and modulate optimal cell behavior …”

Section: Introductionmentioning

confidence: 99%

“…1 The design of biomaterials enables to encapsulate biomolecules effectively, release them significantly, and enhance scaffolds regenerative capacity. 2,3 With this regard, some strategies have focused on the appropriate design and development of biomaterial scaffolds that are incorporated with biomolecules to regulate their release profiles and modulate optimal cell behavior. [4][5][6] Electrospun nanofibers are regarded as a promising ECMmimicking platform and the effective delivery system of biomolecules which can provide physical support for cellular growth to modulate tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

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Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

Ghasemkhah

Latifi

Hadjizadeh

et al. 2019

J Biomedical Materials Res

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The biomaterials design as core–shell structures opens a new door to the release of susceptible biomolecules in a controllable manner and enables to place natural biomaterials as shell layers to impart the effective biofunctional features at surfaces. In this study, core–shell designed scaffolds were prepared using coaxial electrospinning with hybrid of gelatin (GT)/polycaprolactone (PCL) at different weight ratios as their shell and protein solution as their core, followed by cross‐linking to impart controllable release rates, tunable mechanical properties, and enhanced cytocompatibility. SEM, FM, and TEM confirmed the successful production of uniform core–shell nanofibers and homogeneous protein distribution. Results showed that an increase in GT proportion in the shell resulted in a decrease in fiber diameter, an increase of Young's modulus, and an intense burst release of BSA 0.2% which could be controlled through cross‐linking. The mechanical tests revealed that the GT/PCL combining and cross‐linking improved mechanical properties which correlated with an increase in spreading and proliferation of HUVECs. A slight burst release was also detected from BSA 0.05% and EGF encapsulated GT73P‐cross‐linked scaffold which demonstrated their applicability for a controlled release of dilute proteins. We were able to successfully incorporate two types of protein with different concentrations without supporting polymer into the GT shell to provide scaffolds possessing tunable mechanical properties and controllable release rates through blending with PCL at different ratios and/or cross‐linking. These findings are promising to promote delivery systems of angiogenic growth factors that are needed a sustained release with different rates at each angiogenesis stage. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

Ghasemkhah

Latifi

Hadjizadeh

et al. 2019

J Biomedical Materials Res

Self Cite

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“…Unfortunately, this polymer has a low stability in physiological conditions, which is why several studies evaluated the influence of other substances added into gelatin-based biomaterials. (Azizian et al, 2018).…”

Section: Gelatin Propertiesmentioning

confidence: 99%

“…Regarding the in vitro tests, the samples containing bovine serum albumin and basic fibroblast growth factor assured a higher cell viability and an enhanced proliferation of normal human dermal fibroblasts. The authors suggested that these scaffolds are suitable for tissue engineering applications, especially when angiogenesis is needed, and that the stability of these biomaterials could be enhanced by adding crosslinkers (Azizian et al, 2018). The angiogenesis is also important in bone tissue regeneration.…”

Section: New Approaches For Gelatin-based Biomaterialsmentioning

confidence: 99%

“…gelatin-chitosan-bioactive glass low compressive strength, enhanced hMSCs attachment, proliferation, and differentiation, and good in vivo results (Dasgupta et al, 2019) bioactive glass-gelatin methacryloylchitosan higher stiffness for semi-interpenetrating hydrogel networks; increase of CS concentrationincrease of Young's and compressive modulus; (Suo et al, 2018) chitosan-gelatin-bioactive glass suitable for drug and cell encapsulation, good human osteosarcoma cell line viability (Moreira et al, 2018) chitosan-gelatin-chitosan nanoparticles and bovine serum albumin and basic fibroblast growth factor high swelling ratio and water uptake capacity, high cell viability and enhanced proliferation of normal human dermal fibroblasts, enhanced angiogenesis; (Azizian et al, 2018) Strontium-gelatin-bioactive glass high stiffness, ALP activity, and an enhanced angiogenesis (Zare Jalise et al, 2018)…”

Section: Gelatin-based Biomaterialsmentioning

confidence: 99%

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A review of chitosan-, alginate-, and gelatin-based biocomposites for bone tissue engineering

2018

Biomater Tissue Eng Bull

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Bone diseases and injuries have a major impact on the quality of life. Classical treatments for bone repair/regeneration/replacement have various disadvantages. Bone tissue engineering (BTE) received a great attention in the last years. Natural polymers are intensively studied in this field due to their properties (biocompatibility, biodegradability, abundance in nature, high processability). Unfortunately, their mechanical properties are poor, which is why synthetic polymers or ceramics are added in order to provide the optimal compressive, elastic or fatigue strength. Moreover, growth factors, vitamins, or antimicrobial substances are also added to enhance the cell behavior (attachment, proliferation, and differentiation). In this review, new scientific results regarding potential applications of chitosan-, alginate-, and gelatin based biocomposites in BTE will be provided, along with their in vitro and/or in vivo tests.

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Scaffold Fabrication Technologies and Structure/Function Properties in Bone Tissue Engineering

Collins

Ren

Young

et al. 2021

Adv Funct Materials

496

254

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Bone tissue engineering (BTE) is a rapidly growing field aiming to create a biofunctional tissue that can integrate and degrade in vivo to treat diseased or damaged tissue. It has become evident that scaffold fabrication techniques are very important in dictating the final structural, mechanical properties, and biological response of the implanted biomaterials. A comprehensive review of the current accomplishments on scaffold fabrication techniques, their structure, and function properties for BTE is provided herein. Different types of biomaterials ranging from inorganic biomaterials to natural and synthetic polymers and related composites for scaffold processing are presented. Emergent scaffolding techniques such as electrospinning, freeze-drying, bioprinting, and decellularization are also discussed. Strategies to improve vascularization potential and immunomodulation, which is considered a grand challenge in BTE scaffolding, are also presented.

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Chitosan-gelatin porous scaffold incorporated with Chitosan nanoparticles for growth factor delivery in tissue engineering

Cited by 113 publications

References 42 publications

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

A review of chitosan-, alginate-, and gelatin-based biocomposites for bone tissue engineering

Scaffold Fabrication Technologies and Structure/Function Properties in Bone Tissue Engineering

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