Chitosan functionalized poly (ε-caprolactone) nanoparticles for amphotericin B delivery

Marcano, Rossana Gabriela Del Jesús Vásquez; Tominaga, Tânia Toyomi; Khalil, Najeh Maissar; Pedroso, Fiona L.; Mainardes, Rubiana Mara

doi:10.1016/j.carbpol.2018.08.142

Cited by 56 publications

(25 citation statements)

References 62 publications

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“…6 where Qt is the amount of AMB release in time t, K is the release constant, and t is the time. This kinetic model shows that a drug homogeneously dispersed and dissolved in a polymer matrix tends to diffuse through the membrane [50]. Triton was used as a positive control.…”

Section: Analysis Of Amb Encapsulation Efficiency and Aggregationmentioning

confidence: 99%

“…Chitosan functionalized nanoparticles were tested at concentration of 12.5 and 25 g/mL AMB by 6, 24 and 4κ h. The nanoparticles presented negligible disruption of blood cells (<10%) independent of concentration or time [50]. Sulfonated chitosan nanoparticles caused <5% hemolysis for the range (20 to κ0 g/mL) of AMB tested [51].…”

Section: Biocompatibility Assessmentmentioning

confidence: 99%

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Nanocapsules of Sterculia striata acetylated polysaccharide as a potential monomeric amphotericin B delivery matrix

Sombra¹,

Richter²,

Araújo

et al. 2019

International Journal of Biological Macromolecules

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Stable oil nanocapsules based on acetylated Sterculia striata polysaccharide (ASSP) were produced without the use of a surfactant, and derivatives of ASSP with four different degrees of substitution (DS) were synthesised. The data revealed that only derivatives with high DS were able to produce nanocapsules (NC), which exhibited monomodal size distribution profiles with a Z-average particle size,-potential, and polydispersity index (PDI) that were dependent on ASSP DS and concentration. Nanocapsules were loaded with amphotericin B (AMB) with encapsulation efficiencies (EE%) thatwere dependent on drug and ASSP concentrations and DS. A maximum EE% value of 99.2%was achieved, and the loaded AMBwas found to be in amonomeric form, evenwith a concentration one hundredfold higher than that usually observed for commercial AMB aqueous solutions. Loaded nanocapsules show an in vitro controlled release of AMB. As the monomeric AMB state decreased drug toxicity, ASSP nanocapsules loaded with AMB (NC1.68) have potential for use as a drug delivery system. AMB loaded NC 1.68 keeps its activity against 5 strains of Candida albicans tested.

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Section: Analysis Of Amb Encapsulation Efficiency and Aggregationmentioning

confidence: 99%

Section: Biocompatibility Assessmentmentioning

confidence: 99%

Nanocapsules of Sterculia striata acetylated polysaccharide as a potential monomeric amphotericin B delivery matrix

Sombra¹,

Richter²,

Araújo

et al. 2019

International Journal of Biological Macromolecules

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“…A variety of different stabilizers have been exploited up till now with PCL nanoparticles. These stabilizers include nonionic surfactants such as Tween 80 [13] and polymeric stabilizers such as Pluronic F68 [14], Pluronic F127 [15], and polyvinyl alcohol [16]. Such excipients are expected to influence product stability, release behavior, and safety.…”

Section: Introductionmentioning

confidence: 99%

Long-Acting Paliperidone Parenteral Formulations Based on Polycaprolactone Nanoparticles; the Influence of Stabilizer and Chitosan on In Vitro Release, Protein Adsorption, and Cytotoxicity

et al. 2020

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Long-acting preparations containing the antipsychotic paliperidone for intramuscular injection has drawn considerable attention to achieve harmless long-term treatment. This study aimed to develop paliperidone loaded polycaprolactone (PCL) nanoparticles and investigate the influence of PCL/drug ratio, stabilizer type, and chitosan coating on physicochemical properties, protein adsorption, and cellular toxicity. Results showed that chitosan coating produced enlarged particle sizes, shifted the surface charges from negative into positive and did not influence encapsulation efficiencies. Chitosan coating relatively sustained the drug release especially in pluronic stabilized formulations. Pluronic F127 based formulations exhibited the least protein adsorption (384.3 μg/mL). Chitosan coating of Tween 80 and polyvinyl alcohol stabilized formulations significantly (p < 0.05) increased protein adsorption. Cellular viability was concentration-dependent and negatively affected by stabilizers. All formulations did not show cellular death at 1.56 μg/mL. Inflammatory responses and oxidative stress were less affected by Tween 80 compared with other stabilizers. Chitosan minimized all aspects of cellular toxicity. Collectively, stabilizer type and chitosan coating play critical roles in developing safe and effective long-acting PCL nanoparticles intended for parenteral drug delivery. The coated formulations containing Tween 80 and Pluronic F127 as stabilizers are warranted a future in vivo study to delineate its safety and efficacy profiles.

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“…The AmB has a high hemotoxicity even at low concentrations, due to the aggregate conformation (31). Conjugates and ionic cross-linked polymeric nanoparticles of AmB with alginate show less hemotoxicity and greater hemocompatibility than free AmB, possibly due to the protective effect of the polysaccharide and the change in conformation of the super-aggregated state (4,8).…”

Section: Toxicity Studymentioning

confidence: 99%

Alginate-amphothericin B Nanocomplexes Covered by Nanocrystals From Bacterial Cellulose: Physico-chemical Characterization and in Vitro Toxicity

Vs¹,

Silva-Carvalho²,

Martins³

et al. 2020

Preprint

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Background: Nanostructured systems free of surfactants have pharmacotechnical and biopharmaceutical advantages, in addition to being a green process. Amphotericin B (AmB) is a drug with anti-leishmanial and anti-fungal potential, but its low water solubility and permeability limit its therapeutic use. Therefore, it could profit from being incorporated into nanostructured systems. In the present study, a self-assembled nanocomplex of alginate (Alg), a polysaccharide extracted from natural sources that can be used in the pharmaceutical area, with AmB was produced in order to improve the limited therapeutic use of this drug (Alg-AmB). Further, as a reinforcing component, cellulose nanocrystals (NCC) were ionically adsorbed into the surface of the nanocomplex systems (Alg-AmB + NCC). Results: Despite some polydispersity (0.523 ± 0.073), this straightforward process allowed to obtain water soluble particles with a hydrodynamic size of 258.87 ± 10.41 nm and charge of -62.93 ± 2.02 mV. Furthermore, the ionic adsorption of the NCC into the Alg-AmB nanocomplex surface was confirmed by an increase in the particle size (466.3 ± 17.57 nm) and a small surface charge decrease (-55.75 ± 1.23 mV). The amorphous inclusion complex of AmB into the polysaccharide chain network in both formulations was confirmed by DSC and FTIR. AmB in the nanocomplexes was in supper-aggregated form and showed good biocompatibility, being significantly less cytotoxic in vitro against kidney cells and significantly less hemolytic comparatively to the free-drug. Conclusions: The results were indicated the Alg-AmB nanocomplex can be considered an economical, non-toxic alternative to improve the AmB therapeutic effect. Furthermore, NCC coating of the nanocomplexes brought additional protection to the system without compromised the advantages attributed to the developed formulation.

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Chitosan functionalized poly (ε-caprolactone) nanoparticles for amphotericin B delivery

Cited by 56 publications

References 62 publications

Nanocapsules of Sterculia striata acetylated polysaccharide as a potential monomeric amphotericin B delivery matrix

Nanocapsules of Sterculia striata acetylated polysaccharide as a potential monomeric amphotericin B delivery matrix

Long-Acting Paliperidone Parenteral Formulations Based on Polycaprolactone Nanoparticles; the Influence of Stabilizer and Chitosan on In Vitro Release, Protein Adsorption, and Cytotoxicity

Alginate-amphothericin B Nanocomplexes Covered by Nanocrystals From Bacterial Cellulose: Physico-chemical Characterization and in Vitro Toxicity

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