Chitosan from shrimp shell (Crangon crangon) and fish scales (Labeorohita): Extraction and characterization

Abstract

Chitosan is a naturally available biopolymer. It has been prepared by alkaline N-deacetylation process of shrimp (Crangon crangon) chitin and fish (Labeorohita) chitin. The physico-chemical properties such as the degree of deacetylation (DD), solubility, water binding capacity, fat binding capacity and chitosan yield have indicated that shrimp shell and fish scale waste are good sources of chitosan. The deacetylation value of shrimp shell chitosan, fish scales and commercial chitosan was found to be 76, 80 and 84%, respectively. The crystalline index (CrI) of fish and shrimp shell was 84 and 82%. Fat binding capacity of fish chitosan, shrimp chitosan and commercial chitosan was found to be 226, 246 and 446%, respectively. Fourier transforms infrared spectroscopy (FTIR) spectra presented a detailed structure of α-chitin with O-H, N-H and CO stretching movements. Structural differences between shrimp chitosan and fish chitosan were studied by using FTIR, thermo-gravimetric analysis (TGA), Xray powder diffraction (XRD) and scanning electron microscopy (SEM). FTIR spectra were used to determine the chitosan degree of deacetylation (DD). Characteristic properties of extracted chitosan were found to depend upon the source of origin and degree of deacetylation.

Physico-chemical properties of chitosan extracted from Whiteleg shrimp (Litopenaeus vannamei) processing shell waste in Sri Lanka

“…Formation of either dimer with cis-or trans-conformation of secondary amides may have caused these multiple bands as suggested by Ghimire et al (2011). In the chitin FTIR spectrum, amide-I band has been split into two components at 1,647 cm -1 and 1,637 cm -1 confirming that the extracted chitin had α form in major (Kumirska et al 2010;Kumari et al 2015;Suneeta et al 2016). Two types of hydrogen bonds formed by amide groups in the antiparallel alignment present in αchitin crystalline regions give rise to doublet in amide-I band.…”

In Vitro Study on Collagen Application in Wound Healing: A Systematic Review

“…Chitosan is a derivative of chitin that can be extracted from exoskeletons of arthropods such as crabs, horseshoe crab, shrimp, fish scales and from the cell wall of some fungi. [25][26][27][28] It is known to have a haemostatic property for controlling bleeding wounds and increase tissue granulation in the proliferation phase of wound healing. 29,30 However, this functional biopolymer is reported to have poor solubility, and to overcome this problem, the chitosan is hydrolyzed into chitooligosaccharide (COS) to make it soluble in water due to their shorter chain lengths and free amino groups in the D-glucosamine unit.…”

PENGARUH RASIO MASSA KITIN/NaOH DAN WAKTU REAKSI TERHADAP KARAKTERISTIK KITOSAN YANG DISINTESIS DARI LIMBAH INDUSTRI UDANG KERING

“…Kitosan adalah poli-(2-amino-2-deoksi-β-(1-4)-Dglukopiranosa) dihasilkan dari kitin melalui proses deasetilasi dengan menghilangkan gugus asetil (CH 3 -CO) dengan atom hidrogen (H) atau mengubah gugus asetamida (-NHCOCH 3 ) pada kitin menjadi gugus amina (NH 2 ) [1,2]. Kitin terdiri dari rantai lurus gugus acetylglucosamine, merupakan polisakarida dengan ikatan β-glikosidik (1,4) [3,4].…”

“…A = Absorbansi PO = % transmitans pada garis dasar, dengan panjang gelombang 1.655cm -1 atau 3.450 cm -1 P = % transmitans pada puncuk minimun dengan panjang gelombang1.655cm -1 atau 3.450 cm -1 Perbandingan absorbansi pada 1.655 cm -1 dengan absorbansi 3.450 cm -1 digandakan satu per standar N-deasetilasi kitosan (1,33…”