Chitosan disrupts biofilm formation and promotes biofilm eradication in Staphylococcus species isolated from bovine mastitis

Felipe, Verónica; Breser, María Laura; Bohl, Luciana Paola; Silva, Elizabete Rodrigues da; Morgante, Carolina; Correa, Silvia G.; Porporatto, Carina

doi:10.1016/j.ijbiomac.2018.12.159

Cited by 72 publications

(34 citation statements)

References 37 publications

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“…The literature indicates that the NQo MICs and the Qo itself against Pseudomonas aeruginosa vary from 128 to 4096 µg/mL [ 44 ], indicating that the results obtained in this research are favorable due to the range in which they are. Most importantly, in BM related pathogens, NQo MIC values ranged from 200 to 400 μg/mL for S. aureus strains isolated from BM [ 45 ]. The results shown in Figure 3 , indicate that NQo, in comparison to conventional antibiotics used in the treatment of Pseudomonas infections, are equally or around 15% more effective at its antimicrobial effect.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

et al. 2020

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Bovine mastitis (BM) is the most prevalent bacterial infection in the livestock sector, affecting the dairy industry greatly. The prevention and treatment of this disease is mainly made via antibiotics, but the increasing antimicrobial resistance of pathogens has affected the efficiency of conventional drugs. Pseudomonas sp. is one of the pathogens involved in this infection. The therapeutic rate of cure for this environmental mastitis-causing pathogen is practically zero, regardless of treatment. Biofilm formation has been one of the main virulence mechanisms of Pseudomonas hence presenting resistance to antibiotic therapy. We have manufactured chitosan nanoparticles (NQo) with tripolyphosphate (TPP) using ionotropic gelation. These NQo were confronted against a Pseudomonas sp. strain isolated from milk samples of cows diagnosed with BM, to evaluate their antimicrobial and antibiofilm capacity. The NQo showed great antibacterial effect in the minimum inhibitory concentrations (MIC), minimum bactericidal concentration (MBC) and disk diffusion assays. Using sub lethal concentrations, NQo were tested for inhibition of biofilm formation. The results show that the nanoparticles exhibited biofilm inhibition and were capable of eradicate pre-existing mature biofilm. These findings indicate that the NQo could act as a potential alternative to antibiotic treatment of BM.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

et al. 2020

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“…It was majorly reported to inhibit growth and biofilm formation of Staphylococcus spp. causing bovine mastitis [27,122]. Chitosan in nanoparticle form exhibit higher antimicrobial and anti-biofilm capacity than the native chitosan [123].…”

Section: Othersmentioning

confidence: 99%

Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments — A review

Cheng

Han

2020

Asian-Australas J Anim Sci

273

205

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Bovine mastitis, an inflammation of the mammary gland, is the most common disease of dairy cattle causing economic losses due to reduced yield and poor quality of milk. The etiological agents include a variety of gram-positive and gram-negative bacteria, and can be either contagious (e.g., Staphylococcus aureus, Streptococcus agalactiae, Mycoplasma spp.) or environmental (e.g., Escherichia coli, Enterococcus spp., coagulase-negative Staphylococcus, Streptococcus uberis). Improving sanitation such as enhanced milking hygiene, implementation of post-milking teat disinfection, maintenance of milking machines are general measures to prevent new cases of mastitis, but treatment of active mastitis infection is dependant mainly on antibiotics. However, the extensive use of antibiotics increased concerns about emergence of antibiotic-resistant pathogens and that led the dairy industries to reduce the use of antibiotics. Therefore, alternative therapies for prevention and treatment of bovine mastitis, particularly natural products from plants and animals, have been sought. This review provides an overview of bovine mastitis in the aspects of risk factors, control and treatments, and emerging therapeutic alternatives in the control of bovine mastitis.

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“…In recently reported studies, Chitosan, a polysaccharide (Felipe et al ., 2019), and phospholipases such as sPLA2 and PLA2G1B (Seroussi et al ., 2018) have shown promising results for the treatment of mastitis infections caused by various bacterial pathogens. Chitosan has shown the potential to affect the biofilm formation and thus can be used in the control and treatment of bovine mastitis caused by Staphylococcus species (Felipe et al ., 2019). Another study also showed positive results in in vitro analysis.…”

Section: Treatmentmentioning

confidence: 99%

Causes, types, etiological agents, prevalence, diagnosis, treatment, prevention, effects on human health and future aspects of bovine mastitis

Ashraf

Imran

2020

Anim. Health. Res. Rev.

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Mastitis is among the most common and challenging diseases of dairy animals. It is an inflammation of udder tissues due to physical damage, chemical irritation, or infection caused by certain pathogens. Bovine mastitis has been known for ages, but its complex etiology and multi-factorial nature make it difficult to control. Mastitis may have a negative impact on human health by inducing antibiotic-resistant pathogens that may spread, which is threatening. Researchers are continuously struggling to devise suitable methods for mastitis control. Management strategies are mainly focused on disease prevention by farm management which includes proper hygiene, trained staff to monitor minor changes in the udder or milk, and better diagnostic and treatment methods. New technologies which have the potential to unravel this complicated disease include improved diagnostic tools, based on advanced genomics or proteomics, prevention, based on vaccines and immune modulators, and metabolic products of probiotics such as bacteriocins and gene therapy.

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Chitosan disrupts biofilm formation and promotes biofilm eradication in Staphylococcus species isolated from bovine mastitis

Cited by 72 publications

References 37 publications

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments — A review

Causes, types, etiological agents, prevalence, diagnosis, treatment, prevention, effects on human health and future aspects of bovine mastitis

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