Chitosan-coated pore wall polycaprolactone three-dimensional porous scaffolds fabricated by porogen leaching method for bone tissue engineering: a comparative study on blending technique to fabricate scaffolds

Poddar, Deepak; Majood, Misba; Singh, Ankita; Mohanty, Sujata; Jain, Purnima

doi:10.1007/s40204-021-00172-5

Cited by 7 publications

(3 citation statements)

References 62 publications

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“…While there are many scaffold fabrication techniques like emulsion freeze drying ( Sultana and Wang, 2008 ; Qian and Zhang, 2011 ; Sultana and Wang, 2012 ), gas foaming ( Manavitehrani et al, 2019 ; Luo et al, 2021 ; Chen Y et al, 2021 ), porogen leaching ( Bhaskar et al, 2018 ; Poddar et al, 2021 ; Santos-Rosales et al, 2021 ) or phase separation ( Ferrolino et al, 2018 ; Abzan et al, 2019 ; Zeinali et al, 2021 ), more recently additive manufacturing (AM) gained an increased interest for scaffold fabrication ( Shick et al, 2019 ; Szymczyk-Ziółkowska et al, 2020 ; Veeman et al, 2021 ). Generally, the term AM refers to a group of additive manufacturing techniques used to produce a scale model of a physical part or assembly as a three-dimensional model as quickly and inexpensively as possible.…”

Section: Introductionmentioning

confidence: 99%

Recent advances in melt electro writing for tissue engineering for 3D printing of microporous scaffolds for tissue engineering

Loewner

Heene

Baroth

et al. 2022

Front. Bioeng. Biotechnol.

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Melt electro writing (MEW) is a high-resolution 3D printing technique that combines elements of electro-hydrodynamic fiber attraction and melts extrusion. The ability to precisely deposit micro- to nanometer strands of biocompatible polymers in a layer-by-layer fashion makes MEW a promising scaffold fabrication method for all kinds of tissue engineering applications. This review describes possibilities to optimize multi-parametric MEW processes for precise fiber deposition over multiple layers and prevent printing defects. Printing protocols for nonlinear scaffolds structures, concrete MEW scaffold pore geometries and printable biocompatible materials for MEW are introduced. The review discusses approaches to combining MEW with other fabrication techniques with the purpose to generate advanced scaffolds structures. The outlined MEW printer modifications enable customizable collector shapes or sacrificial materials for non-planar fiber deposition and nozzle adjustments allow redesigned fiber properties for specific applications. Altogether, MEW opens a new chapter of scaffold design by 3D printing.

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Section: Introductionmentioning

confidence: 99%

Recent advances in melt electro writing for tissue engineering for 3D printing of microporous scaffolds for tissue engineering

Loewner

Heene

Baroth

et al. 2022

Front. Bioeng. Biotechnol.

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“…All the hemolysis ratio of the dGQH scaffolds (dGQH 20 : 0.65 ± 0.35% dGQH 30 : 0.43 ± 0.17%, dGQH 40 : 0.16 ± 0.29%) were less than 1%, which were much lower than that of dGQ scaffold (1.72 ± 0.57%) (figure 5(H)). Many bone-tissue scaffolds had been reported that the hemolysis ratios were higher than 2% [79][80][81][82], even though the acceptable range is less than 5% [83,84]. In contrast, the hemolysis ratios of the dGQH scaffolds in this study were all much less than 1%, which demonstrated that the dGQH scaffolds had excellent hemocompatibility for bone tissue engineering.…”

Section: Hydrophilicity Degradation and Hemocompatibility Of The Scaf...mentioning

confidence: 47%

3D bioprinting of dECM/Gel/QCS/nHAp hybrid scaffolds laden with mesenchymal stem cell-derived exosomes to improve angiogenesis and osteogenesis

Kang¹,

Xu²,

Meng³

et al. 2023

Biofabrication

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Craniofacial bone regeneration is a coupled process of angiogenesis and osteogenesis, which, associated with infection, still remains a challenge in bone defects after trauma or tumor resection. 3D tissue engineering scaffolds with multifunctional-therapeutic properties can offer many advantages for the angiogenesis and osteogenesis of infected bone defects. Hence, in the present study, a microchannel networks-enriched 3D hybrid scaffold composed of decellularized extracellular matrix (dECM), gelatin (Gel), quaterinized chitosan (QCS) and nano-hydroxyapatite (nHAp) (dGQH) was fabricated by an extrusion 3D bioprinting technology. And enlightened by the characteristics of natural bone microstructure and the demands of vascularized bone regeneration, the exosomes (Exos) isolated from human adipose derived stem cells as angiogenic and osteogenic factors were then co-loaded into the desired dGQH20 hybrid scaffold based on an electrostatic interaction. The results of the hybrid scaffolds performance characterization showed that these hybrid scaffolds exhibited an interconnected pore structure and appropriate degradability (>61% after 8 weeks of treatment), and the dGQH20 hybrid scaffold displayed the highest porosity (83.93 ± 7.38%) and mechanical properties (tensile modulus: 62.68 ± 10.29 MPa, compressive modulus: 16.22 ± 3.61 MPa) among the dGQH hybrid scaffolds. Moreover, the dGQH20 hybrid scaffold presented good antibacterial activities (against 94.90 ± 2.44% of Escherichia coli and 95.41 ± 2.65% of Staphylococcus aureus, respectively) as well as excellent hemocompatibility and biocompatibility. Furthermore, the results of applying the Exos to the dGQH20 hybrid scaffold showed that the Exo promoted the cell attachment and proliferation on the scaffold, and also showed a significant increase in osteogenesis and vascularity regeneration in the dGQH@Exo scaffolds in vitro and in vivo. Overall, this novel dECM/Gel/QCS/nHAp hybrid scaffold laden with Exo has a considerable potential application in reservation of craniofacial bone defects.

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“…Further, the 20% w/v PMS was physically mixed with CHA mixture and molded into 3D scaffolds using the already mentioned syringe molding technique. [ 40 ] In brief, the chitosan and MALD suspension was placed in an 8 mm diameter syringe with the removed end of the needle and fixed with nylon filter paper (pore sizes of 50 µm) at the syringe head, the piston was gently pushed down to extrude the chitosan solution from the syringe, and scaffolds were removed when no more extraction of the solution was observed and dried in a vacuum oven at 37 °C for 48 h. As a second step, CHA‐coated PMS scaffolds were immersed in PCL solution (10% w/v in acetone) and held and reserved at a low vacuum for 48 h to fill the interstitial of the layer with PCL solution. Next, the scaffolds were removed from the PCL solution and washed with 1–4 dioxane to remove any excess PCL solution from the surface.…”

Section: Methodsmentioning

confidence: 99%

Modified‐Hydroxyapatite‐Chitosan Hybrid Composite Interfacial Coating on 3D Polymeric Scaffolds for Bone Tissue Engineering

Poddar,

Singh,

Rao

et al. 2023

Macromolecular Bioscience

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Three‐dimensional scaffolds have huge limitations due to their low porosity, mechanical strength, and lack of direct cell‐bioactive drug contact. Whereas a drug like alendronate's ability to stimulate osteogenesis in osteoblasts and bone marrow mesenchymal stem cells has attracted its therapeutic use, it is hard to administer due to its low bioavailability, quick onset, and lack of site‐specificity. The proposed scaffold architecture allows cells to access the bioactive surface at their apex by interacting at the scaffold's interfacial layer. The interface has been coated with alendronate‐modified hydroxyapatite (MALD) enclosed in a chitosan matrix, the inner pore wall structure of 3D polycaprolactone (PCL) scaffolds to mimic the native environment and provide the direct interaction of cell to bioactive layer and mechanical strength will be provided by the skeleton of PCL. The MALD composite's HAP component will govern ALD's behavior, and local calcium ion concentration increases hMSC proliferation and differentiation. MALD showed total release of 86.28±0.22, and particle size of 273.7±108 nm. XPS and SEM investigation of the 3‐D PCL scaffold structure shows inspiring particle deposition with chitosan over the interface and reinforcing. All scaffolds enhanced cell adhesion, proliferation, and osteocyte differentiation for over a week without in vitro cell toxicity with 3.03±0.2 kPa mechanical strength.This article is protected by copyright. All rights reserved

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Chitosan-coated pore wall polycaprolactone three-dimensional porous scaffolds fabricated by porogen leaching method for bone tissue engineering: a comparative study on blending technique to fabricate scaffolds

Cited by 7 publications

References 62 publications

Recent advances in melt electro writing for tissue engineering for 3D printing of microporous scaffolds for tissue engineering

Recent advances in melt electro writing for tissue engineering for 3D printing of microporous scaffolds for tissue engineering

3D bioprinting of dECM/Gel/QCS/nHAp hybrid scaffolds laden with mesenchymal stem cell-derived exosomes to improve angiogenesis and osteogenesis

Modified‐Hydroxyapatite‐Chitosan Hybrid Composite Interfacial Coating on 3D Polymeric Scaffolds for Bone Tissue Engineering

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