Recent advances in melt electro writing for tissue engineering for 3D printing of microporous scaffolds for tissue engineering

Loewner, Sebastian; Heene, Sebastian; Baroth, Timo; Heymann, Henrik; Cholewa, Fabian; Blume, Holger; Blume, Cornelia

doi:10.3389/fbioe.2022.896719

Cited by 28 publications

(33 citation statements)

References 100 publications

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“…The jet speed ranged from 370 to 500 mm min −1 and was determined by measuring the critical translation speed (CTS), where direct‐written fiber first deposited in a straight line after increasing the collector speed. [ 36 ] All samples were printed at 1.1× CTS to ensure straight fiber printing while avoiding too much stretching. Different G‐codes were generated for the printing of the structures (Figure 1b–g).…”

Section: Methodsmentioning

confidence: 99%

Magnetically Responsive Melt Electrowritten Structures

Saiz

Reizabal

Luposchainsky

et al. 2023

Adv Materials Technologies

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While melt electrowriting (MEW) can result in complex microstructures, research demonstrating such fabrication with active materials is limited. Herein, magnetoresponsive poly(𝝐-caprolactone) (PCL) inks containing up to 10 wt% of iron-oxide (Fe 3 O 4 ) nanoparticles are used to produce fiber with diameters of 9.2 ± 0.6 μm in ordered microstructures when processed by MEW. Introducing the Fe 3 O 4 nanoparticles has a minimal overall effect on printing quality compared to pure PCL under similar conditions. The magnetic response of Fe 3 O 4 containing fibers allows magnetic actuation, which is one of the first steps to control movement in such structures. Printed samples show different magnetic responses that can be controlled by the micro-and macro-structure design, the nanoparticle concentration, and multi-material design. The potential of MEW to print active magnetic complex micro-and macro-structures for 4D printing designs is demonstrated, in which active properties can be further tailored with magnetoresponsive fillers with varying characteristics and by changing MEW fiber diameters.

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Section: Methodsmentioning

confidence: 99%

Magnetically Responsive Melt Electrowritten Structures

Saiz

Reizabal

Luposchainsky

et al. 2023

Adv Materials Technologies

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“…However, this effect can be minimized in the melt-based electro-jetting system; thus, a straight microscale polymeric thread is used to build the micro-3D structure without the whipping motion of the thread. [20][21][22][23] The advantage of the melt-based system for biological applications is the ability to use micro-3D structure without further purifications, such as removing solvents and additives, rendering the material more cell compatible. Figure 2b shows macroscopic light images of printed PCL scaffolds being handled.…”

Section: Large-scale Shear-induced Fn Fibrillogenesis Using a Fluidic...mentioning

confidence: 99%

A Scalable Engineered Extracellular Matrix Platform to Expand Tumor Cells

Moon

Neale

Kim

et al. 2023

Advanced NanoBiomed Research

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The demand for high‐throughput and scalable cell expansion platforms that can accommodate diverse cell types remains a critical requirement across various biomedical fields. Fibronectin (Fn), an essential component of the extracellular matrix (ECM), has been used as a conformal surface coating for two‐dimensional (2D) cell culture systems. However, the soluble, globular Fn used for 2D coatings differs structurally from the native Fn, which possesses a three‐dimensional (3D) fibrillar structure. Herein, a large‐scale engineered ECM (EECM) cell expansion platform based on a 3D fibrillar Fn network spanning over centimeters is presented. Extended fibrillar networks are formed by shearing dilute Fn solutions over tessellated polymeric scaffolds, which are conveniently prepared by 3D printing. The structure and size of the Fn‐based 3D EECM scaffold are optimized by evaluating the proliferation of a colorectal tumor cell line, CT26, commonly used in the in vivo tumor immunotherapy models. The 3D EECM scaffolds support a fourfold more efficient tumor cell expansion than a conventional 2D culture system, demonstrating the potential efficacy in supporting the robust expansion of cancer cells ex vivo with an eye on cancer immunotherapy.

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“…108 The ratio between the electrical and the mechanical parameters must be considered, as it defines the amount of polymer delivered to the jet that can be accepted for the flow rate provided by the electric field, seeking to avoid defects such as ribbon-like structures or spherical drops. 98,109,110 ES and MEW techniques have been used to manufacture numerous types of biomedical devices applicable in various fields, such as multi-purpose biocompatible scaffolds, 55,[111][112][113][114][115] cartilage, 116 and cardiac [117][118][119] tissue engineering scaffolds, constructs for stem cell therapy 120 or drug delivery devices. [121][122][123][124] Their application in skin tissue engineering scaffold manufacturing is also wide.…”

Section: Biomaterials Description Main Propertiesmentioning

confidence: 99%

The rise of mechanical metamaterials: Auxetic constructs for skin wound healing

Lecina-Tejero,

Pérez,

García-Gareta

et al. 2023

J Tissue Eng

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Auxetic materials are known for their unique ability to expand/contract in multiple directions when stretched/compressed. In other words, they exhibit a negative Poisson’s ratio, which is usually positive for most of materials. This behavior appears in some biological tissues such as human skin, where it promotes wound healing by providing an enhanced mechanical support and facilitating cell migration. Skin tissue engineering has been a growing research topic in recent years, largely thanks to the rapid development of 3D printing techniques and technologies. The combination of computational studies with rapid manufacturing and tailored designs presents a huge potential for the future of personalized medicine. Overall, this review article provides a comprehensive overview of the current state of research on auxetic constructs for skin healing applications, highlighting the potential of auxetics as a promising treatment option for skin wounds. The article also identifies gaps in the current knowledge and suggests areas for future research. In particular, we discuss the designs, materials, manufacturing techniques, and also the computational and experimental studies on this topic.

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Recent advances in melt electro writing for tissue engineering for 3D printing of microporous scaffolds for tissue engineering

Cited by 28 publications

References 100 publications

Magnetically Responsive Melt Electrowritten Structures

Magnetically Responsive Melt Electrowritten Structures

A Scalable Engineered Extracellular Matrix Platform to Expand Tumor Cells

The rise of mechanical metamaterials: Auxetic constructs for skin wound healing

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