Chitosan-coated mesoporous MIL-100(Fe) nanoparticles as improved bio-compatible oral nanocarriers

Abstract

Nanometric biocompatible Metal-Organic Frameworks (nanoMOFs) are promising candidates for drug delivery. Up to now, most studies have targeted the intravenous route, related to pain and severe complications; whereas nanoMOFs for oral administration, a commonly used non-invasive and simpler route, remains however unexplored. We propose here the biofriendly preparation of a suitable oral nanocarrier based on the benchmarked biocompatible mesoporous iron(III) trimesate nanoparticles coated with the bioadhesive polysaccharide chitosan (CS). This method does not hamper the textural/structural properties and the sorption/release abilities of the nanoMOFs upon surface engineering. The interaction between the CS and the nanoparticles has been characterized through a combination of high resolution soft X-ray absorption and computing simulation, while the positive impact of the coating on the colloidal and chemical stability under oral simulated conditions is here demonstrated. Finally, the intestinal barrier bypass capability and biocompatibility of CS-coated nanoMOF have been assessed in vitro, leading to an increased intestinal permeability with respect to the non-coated material, maintaining an optimal biocompatibility. In conclusion, the preservation of the interesting physicochemical features of the CS-coated nanoMOF and their adapted colloidal stability and progressive biodegradation, together with their improved intestinal barrier bypass, make these nanoparticles a promising oral nanocarrier.

GraftFast Surface Engineering to Improve MOF Nanoparticles Furtiveness

“…This indicates an electronic stabilizing effect of the PEG coating over the Fe III species and supports the idea of an interaction between the Fe III species at the surface of the NP and the acryl‐PEG molecules. These results are in agreement with previous studies using chitosan as coating material …”

“…Different methods, used for MOFs surface coating to date comprise either physical adsorption or chemical association. The former exploits electrostatic interactions between the surface and the coating agent and occurs under desired mild conditions, which not only improves nanoMOFs' chemical and colloidal stability but also affects their circulating fate, providing properties such as stealth, targeting, or bioadhesion, without compromising the MOF porosity and therefore, its encapsulation/release capabilities. The second approach involves a selective grafting typically directed through click chemistry, condensation reactions, or chemical complexation, among others, ending with an even more stable surface coating, which also confers multiple properties such as stability, enhanced cytotoxicity, as well as long circulating properties or targeting.…”

Enzyme‐MOF Nanoreactor Activates Nontoxic Paracetamol for Cancer Therapy

“…MOFs are an emerging platform that demonstrates potential for a number of biotechnological applications, including sensing, imaging, drug delivery, and enzyme encapsulation. 20-42 Encapsulated enzymes demonstrate well-preserved catalytic activities. 21, 24, 25 Encapsulated enzymes also show enhanced stability under protein denaturation conditions, such as organic solvents, extreme pH environments, or high temperatures.…”

Some Nano Bio-Mofs Evaluated For Storage Purpose of Drugs

“…This emerging class of nano-bio-MOFs can be considered as promising candidates for the drug adsorption and its controlled release due to their large surface areas, high porosity, and presence of bio compatible linker molecules [11]. Several research reports reveal the use of nano sized MOFs in the field of drugs delivery [12][13][14].…”