Chitosan capped CuInS2 and CuInS2/ZnS by wet stirred media milling: in vitro verification of their potential bio-imaging applications

Dutková, Erika; Bujňáková, Zdenka; Kello, Martin; Mojžiš, Ján; Skurikhina, Olha; Briančin, Jaroslav

doi:10.1007/s13204-020-01530-8

Cited by 5 publications

(6 citation statements)

References 44 publications

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“…[ 26 ] Moreover, fluorescence imaging in the NIR region near 800 nm has been applied in clinical procedures, for example to evaluate vascular flow in grafted tissues in reconstructive surgery, and to diagnose retinal detachments and macular degeneration through retinal angiography. [ 27 ] Only a few types of NIR QDs, however, such as CuInS 2 /ZnS, [ 28 ] PbS, [ 29 ] and CdSeTe QDs, have been considered as candidates for both bio‐imaging and heavy metal detection. [ 30 ] Recently, there have been many reports on detecting metal ions with carbon QDs, [ 31–33 ] but the overwhelming majority of carbon dots only emit in the blue or green region, which severely limits their application in bio‐imaging.…”

Section: Introductionmentioning

confidence: 99%

Assaying of Cu²⁺ with near‐infrared l‐cysteine‐capped CdSeTe/CdS quantum dots and its effect on cell imaging

Zhang

Huang

et al. 2021

Luminescence

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Near‐infrared (NIR) core–shell CdSeTe/CdS quantum dots (QDs) modified with l‐cysteine were synthesized in aqueous solution. The QDs had a special NIR‐emitting spectrum, high fluorescence stability and low cytotoxicity. In addition, they exhibited an obvious fluorescence quenching when Cu2+ was present. An NIR nanosensor was prepared for rapidly, sensitively, and selectively determining Cu2+ in solution quantitatively and monitoring the changes in Cu2+ in cells with fluorescence imaging in a semiquantitative way. The linear relationship between the relative fluorescence intensity (F0/F) and the concentration of Cu2+ from 5.12 × 10−8 M to 2.56 × 10−5 M in solution was observed using an NIR fluorescence spectrophotometer with R2 equal to 0.9958. Moreover, in the experiment with the fluorescence microscope, F0/F versus the concentration of Cu2+ from 5.00 × 10−8 M to 7.68 × 10−6 M also showed a good linear relationship with R2 equal to 0.9817. Practical water sample ion detecting experiments had good accuracy and recovery rates. Cell experiments showed that the NIR imaging intensity of cells was inversely proportional to the concentration of copper ions, therefore NIR QDs have great potential for detection of metal ions in solution and in cells.

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Section: Introductionmentioning

confidence: 99%

Assaying of Cu²⁺ with near‐infrared l‐cysteine‐capped CdSeTe/CdS quantum dots and its effect on cell imaging

Zhang

Huang

et al. 2021

Luminescence

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“…Compared to other systems prepared by the wet milling method in the laboratory circulation mill (MiniCer), these are less demanding conditions. Parameters such as total preparation time, milling speed as well as centrifugation speed were reduced individually by tens of percentin comparison with the conditions used for other systems ( Bujňáková et al, 2017a ; Bujňáková et al, 2020 ; Dutková et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

“…The wet milling method has been used several times for mechanical activation, nanoscale product formation, as well as nanosuspension preparation ( Stenger et al, 2005 ; Niwa et al, 2011 ; Jung et al, 2015 ; Kuroiwa et al, 2018 ; Karuppannan et al, 2019 ). Using this technique, our research group was able to prepare stable nanosuspensions with interesting magnetic, optical and therapeutic properties by combining inorganic materials with bioactive and biocompatible capping agents in the past ( Bujňáková et al, 2017a ; Bujňáková et al, 2017b ; Bujňáková et al, 2020 ; Dutková et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Mechanochemical Preparation, Characterization and Biological Activity of Stable CuS Nanosuspension Capped by Bovine Serum Albumin

et al. 2022

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The biocompatible nanosuspension of CuS nanoparticles (NPs) using bovine serum albumin (BSA) as a capping agent was prepared using a two-stage mechanochemical approach. CuS NPs were firstly synthetized by a high-energy planetary ball milling in 15 min by milling elemental precursors. The stability of nanoparticles in the simulated body fluids was studied, revealing zero copper concentration in the leachates, except simulated lung fluid (SLF, 0.015%) and simulated gastric fluid (SGF, 0.078%). Albumin sorption on CuS NPs was studied in static and dynamic modes showing a higher kinetic rate for the dynamic mode. The equilibrium state of adsorption was reached after 90 min with an adsorption capacity of 86 mg/g compared to the static mode when the capacity 59 mg/g was reached after 2 h. Then, a wet stirred media milling in a solution of BSA was introduced to yield the CuS-BSA nanosuspension, being stable for more than 10 months, as confirmed by photon cross-correlation spectroscopy. The fluorescent properties of the nanosuspension were confirmed by photoluminescence spectroscopy, which also showed that tryptophan present in the BSA could be closer to the binding site of CuS than the tyrosine residue. The biological activity was determined by in vitro tests on selected cancer and non-tumor cell lines. The results have shown that the CuS-BSA nanosuspension inhibits the metabolic activity of the cells as well as decreases their viability upon photothermal ablation.

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“…Several reports are found of biological applications due to the less toxic and ecofriendly nature of CIS NPs. Among them, chitosan-capped CIS NPs have been investigated for bioimaging applications . The CIS/ZnS core–shell nanocrystals are employed as fluorescent probes for in vivo and in vitro biological imaging .…”

Section: Introductionmentioning

confidence: 99%

“…Among them, chitosan-capped CIS NPs have been investigated for bioimaging applications. 26 The CIS/ZnS core−shell nanocrystals are employed as fluorescent probes for in vivo and in vitro biological imaging. 27 A study shows the good biocompatibility stability of Gd-based CIS/ZnS nanoprobes for in vitro cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible CuInS₂ Nanoparticles as Potential Antimicrobial, Antioxidant, and Cytotoxic Agents

Giri

Chaki

Khimani³

et al. 2021

ACS Omega

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A simple hydrothermal route is employed to synthesize pure copper indium disulfide (CIS) and CIS nanoparticles (NPs) mediated by various natural plant extracts. The plant extracts used to mediate are Azadirachta indica (neem), Ocimum sanctum (basil), Cocos nucifera (coconut), Aloe vera (aloe), and Curcuma longa (turmeric). The tetragonal unit cell structure of as-synthesized NPs is confirmed by X-ray diffraction. The analysis by energy-dispersive X-rays shows that all the samples are nearstoichiometric. The morphologies of the NPs are confirmed by high-resolution scanning and transmission modes of electron microscopy. The thermal stability of the synthesized NPs is determined by thermogravimetric analysis. The optical energy band gap is determined from the absorption spectra using Tauc's equation. The antimicrobial activity analysis and the estimation of the minimum inhibitory concentration (MIC) value of the samples are performed for Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Enterobacter aerogenes, and Staphylococcus aureus pathogens. It shows that the aloe-mediated CIS NPs possess a broad inhibitory spectrum. The best inhibitory effect is observed against S. aureus, whereas the least effect was exhibited against P. vulgaris. The least MIC value is found for aloe-mediated CIS NPs (0.300 mg/mL) against S. aureus, P. aeruginosa, and E. aerogenes, along with basil-mediated NPs against E. coli. The antioxidant activity study showed that the IC 50 value to inhibit the scavenging activity is maximum for the control (vitamin C) and minimum for pure CIS NPs. The in vivo cytotoxicity study using brine shrimp eggs shows that the pure CIS NPs are more lethal to brine shrimp than the natural extract-mediated CIS NPs. The in vitro cytotoxicity study using the human lung carcinoma cell line (A549) shows that the IC 50 value of turmeric extract-mediated CIS NPs is minimum (15.62 ± 1.58 μg/mL). This observation reveals that turmeric extract-mediated CIS NPs are the most potent in terms of cytotoxicity toward the A549 cell line.

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Chitosan capped CuInS2 and CuInS2/ZnS by wet stirred media milling: in vitro verification of their potential bio-imaging applications

Cited by 5 publications

References 44 publications

Assaying of Cu²⁺ with near‐infrared l‐cysteine‐capped CdSeTe/CdS quantum dots and its effect on cell imaging

Assaying of Cu²⁺ with near‐infrared l‐cysteine‐capped CdSeTe/CdS quantum dots and its effect on cell imaging

Mechanochemical Preparation, Characterization and Biological Activity of Stable CuS Nanosuspension Capped by Bovine Serum Albumin

Biocompatible CuInS₂ Nanoparticles as Potential Antimicrobial, Antioxidant, and Cytotoxic Agents

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Chitosan capped CuInS2 and CuInS2/ZnS by wet stirred media milling: in vitro verification of their potential bio-imaging applications

Cited by 5 publications

References 44 publications

Assaying of Cu2+ with near‐infrared l‐cysteine‐capped CdSeTe/CdS quantum dots and its effect on cell imaging

Assaying of Cu2+ with near‐infrared l‐cysteine‐capped CdSeTe/CdS quantum dots and its effect on cell imaging

Mechanochemical Preparation, Characterization and Biological Activity of Stable CuS Nanosuspension Capped by Bovine Serum Albumin

Biocompatible CuInS2 Nanoparticles as Potential Antimicrobial, Antioxidant, and Cytotoxic Agents

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Assaying of Cu²⁺ with near‐infrared l‐cysteine‐capped CdSeTe/CdS quantum dots and its effect on cell imaging

Assaying of Cu²⁺ with near‐infrared l‐cysteine‐capped CdSeTe/CdS quantum dots and its effect on cell imaging

Biocompatible CuInS₂ Nanoparticles as Potential Antimicrobial, Antioxidant, and Cytotoxic Agents