Chitosan‐calcium alginate dressing promotes wound healing: A preliminary study

Abstract

Dressings are necessary during the process of wound healing. Since the early 1980s, several types of wound dressings have been produced, but they cannot always take into account some effects include antibacterial effect, wound healing promotion, and other properties. In this study, we would like to develop an effective dressing with the above properties, especially accelerating wound healing effect. A chitosancalcium alginate dressing (CCAD) was developed by coating mixture of chitosan with high-low molecular weight on calcium alginate dressing (CAD). We investigated the structural characteristics of CCAD with Fourier-transform infrared spectroscopy (FTIR) and electron microscopy. The cytotoxicity and antibacterial property were evaluated in vitro using CCK-8 and inhibition zone method. Moisture retention was tested on the skin of Sprague-Dawley (SD) rats, and wound healing studies were performed on a full-thickness skin wound model in SD rats. CCAD showed good moisturizing and antibacterial properties with no cytotoxicity. CCAD could inhibit inflammation by decreasing IL-6, and it could also promote angiogenesis by increasing VEGF, resulting in better wound healing than CAD. CCAD is a better choice in wound care due to its antibacterial property, biocompatibility, moisture retention, healing promotion, and non-cytotoxicity characteristics.

Usnic‐Acid‐Functionalized Silk Fibroin Composite Scaffolds for Cutaneous Wounds Healing

“…Angiogenesis is another important step of wound healing, which can provide necessary nutrition for granulation and surrounding tissues. [ 42,43 ] As mentioned before, angiogenesis was initiated promptly in the USCS group compared with controls, and the potential mechanism was also discussed in this work. Previous studies have shown that the expression of endogenous vascular endothelial growth factor (VEGF) is significantly increased during wound‐healing angiogenesis, especially in the early stage of capillary formation; [ 44,45 ] thus, the time course of VEGF expression level was also determined by immuno‐histochemical analysis on days 3, 7, and 12 after wounding ( Figure 9 ).…”

“…These samples were fixed in 4% paraformaldehyde for at least 24 h, subsequently embedded in paraffin and sectioned into a thickness of 10 µm to undergo routine histological processing with hematoxylin–eosin and Masson's Trichrome staining for histological analysis. [ 24,30,42,46 ] Primary antibodies for CK10, VEGF (Abcam, Cambridge, MA), and SP‐POD kit (Solarbio Science & Technology Co., Ltd.) for immuno‐histochemistry analysis. [ 30,42,44 ] Antigen retrieval was performed on sections under heating, and then incubation with a blocking reagent for 20 min.…”

“…[ 24,30,42,46 ] Primary antibodies for CK10, VEGF (Abcam, Cambridge, MA), and SP‐POD kit (Solarbio Science & Technology Co., Ltd.) for immuno‐histochemistry analysis. [ 30,42,44 ] Antigen retrieval was performed on sections under heating, and then incubation with a blocking reagent for 20 min. The samples were incubated at 4 °C overnight with antimouse CK10 and VEGF (diluted 1:100 in antibody diluent) after removing the blocking reagent.…”

Flexible electrical stimulation device with Chitosan-Vaseline® dressing accelerates wound healing in diabetes

“…By contrast, high MW chitosan (>100,000 Da) inhibits the growth of Gram-positive bacteria and regulates complement-mediated activities [ 39 , 40 ]. In our previous study, we have optimized the properties of chitosan by using a mixture of high MW and low MW forms [ 41 ]. However, chitosan solutions are viscous; dressings prepared with chitosan solution alone will adhere to the wound and promote tears in the granulation tissue during dressing changes that are not conducive to wound healing.…”

Bio-Based Electrospun Fibers for Wound Healing

“…In recent years, considerable advances have been achieved in designing modern dressings to protect the wound from dehydration and infection, and facilitate the healing process instead of just covering the wound [3,11,12]. Wound dressings in the form of hydrogels [13][14][15], hydrocolloids [16][17][18], sponges [19][20][21][22], alginates [23][24][25], and transparent films [11] have been developed and some of them are commercially available. These materials are different in their inherent features, such as hydrophobicity, permeability, and adsorption capacity.…”