Chitosan-Based Nanoencapsulation of Ocimum americanum Essential Oil as Safe Green Preservative Against Fungi Infesting Stored Millets, Aflatoxin B1 Contamination, and Lipid Peroxidation

Nanostructures are usually formed by solvent dissolution, but this paper proposes a green-chemistry method for thymol nano-encapsulation based on a chitosan–gelatin bio-copolymer matrix formation that enhances the physical stability to obtain a thymol-modified release system, with antioxidant capacity. Various ratios of chitosan–gelatin, gelatin types A and B, and crosslinkers were evaluated at a constant thymol concentration of 0.5 mg/ml. Gallic acid was chosen as the crosslinker. All batches were ultrasonicated to reduce particle size. The best conditions were obtained using a chitosan–gelatin ratio of 1:4 with type A gelatin, as those nanoparticles had higher physical stability, together with a smaller particle size (316.5 ± 2 nm) and higher thymol encapsulation efficiency (88 ± 3%). Antioxidant capacity was evaluated by DPPH, ABTS (radical inhibition 87.06 ± 4.38%, and 88.5 ± 4.42%, respectively), and a FRAP assay (1084.68 ± 54.32 µM Trolox equivalents). Release profiles were evaluated at two pH values (5.5, 7.0) and environmental temperatures (4, 25 °C). Diffusion was non-Fickian in all treatments. Gelatin type A systems exhibited a major physical stability, influencing the reduction of released thymol significantly. The research findings suggest that this submicronic dispersion can be used as a modified release system with high antioxidant activity and potentially serve as a preservative system during food storage.

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Nanoemulsification of Essential Oil Blend by Ultrasound: Optimization of Physicochemical, Antioxidant Properties, and Activity Against Escherichia coli

Torres Neto,

Monteiro,

Mutz

et al. 2023

Food Bioprocess Technol

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Chitosan-Based Nanoencapsulation of Ocimum americanum Essential Oil as Safe Green Preservative Against Fungi Infesting Stored Millets, Aflatoxin B1 Contamination, and Lipid Peroxidation

Cited by 6 publications

References 95 publications

Fabrication, Characterization, and Antifungal Assessment of Oregano Essential Oil-Loaded Nano-silica Against Curvularia lunata in Brown Rot of Agaricus bisporus Storage

Fabrication, Characterization, and Antifungal Assessment of Oregano Essential Oil-Loaded Nano-silica Against Curvularia lunata in Brown Rot of Agaricus bisporus Storage

A Green Method for Nanoencapsulation of Thymol in Chitosan–Gelatin with Antioxidant Capacity

Nanoemulsification of Essential Oil Blend by Ultrasound: Optimization of Physicochemical, Antioxidant Properties, and Activity Against Escherichia coli

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