Chitinolytic efficacy and secretion of cell wall degrading enzymes from Trichoderma spp. in response to phyto-pathological fung

doi:10.7324/jabb.2017.50601

Cited by 13 publications

(4 citation statements)

References 33 publications

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“…Chitinase activity is essential for the selection of an efficient biocontrol agent since chitin is the major structural component of cell wall from pathogens. Chitinases together with β-1,3-glucanases are cell-wall-degrading enzymes involved in the initiation of mycoparasitism [42,43]. The secreted enzymes act synergistic each other, but at the same time with other metabolites.…”

Section: Discussionmentioning

confidence: 99%

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Applications of Fungal Strains with Keratin-Degrading and Plant Growth Promoting Characteristics

et al. 2019

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Protein hydrolysates (PHs) are organic non-microbial biostimulants having beneficial effects on plants. The study was designed to assess the effects on plants by the applications of PHs obtained from Trichoderma isolates grown on keratin wastes. Trichoderma isolates were characterized for indole-3-acetic acid and siderophores production, activity of lytic enzymes, phosphorous solubilization and inhibition of pathogens growth, using qualitative specific tests. Fungal isolates were cultured on a medium with keratin wastes (wool and feathers) to obtain PHs. Fungal PHs were tested in vivo for plant biostimulant action, as follows: (i) seeds germination test; (ii) activation of plant proton pump; (iii) evaluation of effect on tomato seedling growth. PHs from T. asperellum cultured on feathers medium reached the highest values for all parameters recorded (plant height and diameter, number of leaves and branches), with the exception of those for plant biomass, which were maximum for the wool medium. The metabolites released by keratin degradation under the activity of selected T. asperellum isolate improved crop health and productivity. The use of PHs can be a reasonable solution for the environmental pollution of by-products from the food chain, as well as for the replacement of chemical fertilizers with microbial formulations to stimulate plant growth.

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Section: Discussionmentioning

confidence: 99%

“…Indole-3-acetic acid is the most common plant hormone belonging to auxin class that regulates various aspects of plant growth and development [7,[43][44][45][46]. IAA is responsible for cell division and elongation responding to various factors as light, presence of pathogens etc.…”

Section: Discussionmentioning

confidence: 99%

Applications of Fungal Strains with Keratin-Degrading and Plant Growth Promoting Characteristics

et al. 2019

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“…Mycoparasitism was observed in the interaction between C. globosum and B. sorokiniana, along with a reduction of the pathogen mycelium growth in the present study. The mode of action in terms of direct interaction including the production of hydrolytic CWDEs as well as biocidal substances is based on a mycotrophic lifestyle of the antagonistic organisms (Qualhato et al, 2013;Khatri et al, 2017), which induces strong hydrolysis of pathogenic cell walls and results in inhibition of spore germination and mycelial digestion (Sun et al, 2005). A similar mechanism of inhibition was reported in Trichoderma spp.…”

Section: Discussionmentioning

confidence: 78%

Transcriptome Profiling Provides Insights Into Potential Antagonistic Mechanisms Involved in Chaetomium globosum Against Bipolaris sorokiniana

et al. 2020

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Chaetomium globosum Kunze is recognized as a potential biocontrol fungus against spot blotch of wheat caused by Bipolaris sorokiniana. Its molecular mechanism of biocontrol activity and the biosynthetic pathways involved have not been yet elucidated. Here, global transcriptome profiling of C. globosum strain Cg2 during interaction with B. sorokiniana isolate BS112 using RNA-seq was performed in order to gain insights into the potential mechanisms of antagonism. The Illumina HiSeq platform (2 × 150 bp) yielded an average of 20–22 million reads with 50–58% GC. De novo assembly generated 45,582 transcripts with 27,957 unigenes. Transcriptome analysis displayed distinct expression profiles in the interaction (Cg2–BS112), out of which 6,109 unique differentially expressed genes were present. The predominant transcripts classified as genes involved in “catalytic activity” constituted 45.06%, of which 10.02% were associated with “hydrolytic activity” (GO:0008152), and similarly, in the biological process, 29.18% of transcripts were involved in “metabolic activity” (GO:0004096 and GO:0006979). Heat map and cluster categorization suggested an increase in the expression levels of genes encoding secondary metabolites like polyketide synthase (GO:0009058), S-hydroxymethyl glutathione dehydrogenase (GO:0006069), terpene cyclase (EC 4.2.3.-), aminotran_1_2 domain-containing protein (GO:0009058), and other hydrolytic CAZYmes such as the glycosyl hydrolase (GH) family (GH 13, GH 2, GH 31, and GH 81; GO:0005975), cellulase domain-containing protein, chitinases, β-1, 3-glucanases (GO:0004565), glucan endo-1,3-beta-glucanase (GO:0052861), and proteases (GO:0004177). The obtained RNA-seq data were validated by RT-qPCR using 20 randomly chosen genes, showing consistency with the RNA-seq results. The present work is worldwide the first effort to unravel the biocontrol mechanism of C. globosum against B. sorokiniana. It generated a novel dataset for further studies and facilitated improvement of the gene annotation models in the C. globosum draft genome.

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“…Moreover, long-term studies by many researchers showed that high antagonism of Trichoderma sp. resulting from the increased production of CDWE enzymes limits the growth of the following pathogens: Corynespora cassiicola, Curvularia aeria [92,93], Sclerotinia sclerotiorum [94], Sclerotium rolfsii [89], Aspergillus niger, Fusarium oxysporum [95,96], Colletotrichum gloeosporioides, Botrytis cinerea [97,98], Aspergillus flavus, Aspergillus fumigatus, Candida albicans, Mucor circinelloides, Rhizoctonia solani [99][100][101], Fusarium graminearum [68], Fusarium fujikuroi, Fusarium tricinctum, and Fusarium cantenulatum [102].…”

Section: Mycoparasitismmentioning

confidence: 99%

Fungi of the Trichoderma Genus: Future Perspectives of Benefits in Sustainable Agriculture

et al. 2023

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The negative impact of chemical pesticides on the environment and human health has contributed to the introduction of legal regulations that ensure the reduction in the use of agrochemicals in favor of biological products. The existing review of the literature, including our research, clearly shows that the ideal biocontrol agents are Trichoderma fungi. The production of antibiotics, lytic enzymes degrading the cell walls of plant pathogens, or inducing a defense response in plants are just some of the features supporting the wide use of these microorganisms in sustainable agriculture. It is estimated that currently about 60% of biofungicides used to eliminate fungal pathogens are produced based on Trichoderma sp. strains.

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Chitinolytic efficacy and secretion of cell wall degrading enzymes from Trichoderma spp. in response to phyto-pathological fung

Cited by 13 publications

References 33 publications

Applications of Fungal Strains with Keratin-Degrading and Plant Growth Promoting Characteristics

Applications of Fungal Strains with Keratin-Degrading and Plant Growth Promoting Characteristics

Transcriptome Profiling Provides Insights Into Potential Antagonistic Mechanisms Involved in Chaetomium globosum Against Bipolaris sorokiniana

Fungi of the Trichoderma Genus: Future Perspectives of Benefits in Sustainable Agriculture

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