Chitin-Lignin Material as a Novel Matrix for  Enzyme Immobilization

Zdarta, Jakub; Kłapiszewski, Łukasz; Wysokowski, Marcin; Norman, Małgorzata; Kołodziejczak-Radzimska, Agnieszka; Moszyński, Dariusz; Ehrlich, Hermann; Maciejewski, Hieronim; Stelling, Allison L.; Jesionowski, Teofil

doi:10.3390/md13042424

Cited by 80 publications

(35 citation statements)

References 71 publications

(73 reference statements)

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“…Lipase from Aspergillus niger has been successfully immobilized on this biopolymer–biopolymer hybrid matrix. The results of the study in question confirmed that the thermal and chemical stability of the immobilized lipase was improved …”

Section: Introductionmentioning

confidence: 66%

Luffa cylindrica sponges as a thermally and chemically stable support for Aspergillus niger lipase

Zdarta

Jesionowski

2016

Biotechnology Progress

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The use of biopolymer compounds as matrices for enzyme immobilization is currently a focus of increasing interest. In the present work we propose the use of Luffa cylindrica vegetable sponges as a support for the lipase extracted from Aspergillus niger. Effectiveness of immobilization was analyzed using Fourier transform infrared spectroscopy, elemental analysis and the Bradford method. An initial enzyme solution concentration of 1.0 mg/mL and an immobilization time of 12 h were selected as the parameters that produce a system retaining the highest hydrolytic activity (84% of free enzyme). The resulting biocatalyst system also exhibited high thermal and chemical stability, reusability and storage stability, which makes it a candidate for use in a wide range of applications. Kinetic parameters for the native and immobilized lipase were also calculated. The value of the Michaelis-Menten constant for the immobilized lipase (0.47 mM) is higher than for the free enzyme (0.21 mM), which indicates that the adsorbed enzyme exhibits a lower affinity to the substrate than native lipase. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:657-665, 2016.

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Section: Introductionmentioning

confidence: 66%

Luffa cylindrica sponges as a thermally and chemically stable support for Aspergillus niger lipase

Zdarta

Jesionowski

2016

Biotechnology Progress

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“…Activation of lipases occurs because of the amphiphilic properties of lignins, which are similar to those of synthetic surfactants . Lipase from Aspergillus niger was adsorbed on a composite material consisting of chitin and oxidized KL . The immobilization increased thermal and pH stability compared to that of native lipase.…”

Section: Entrapment Encapsulation and Adsorption Of Active Substancmentioning

confidence: 99%

Lignin for Nano‐ and Microscaled Carrier Systems: Applications, Trends, and Challenges

et al. 2019

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To liberate society from its dependence on fossil‐based fuels and materials it is pivotal to explore components of renewable plant biomass in applications that benefit from their intrinsic biodegradability, safety, and sustainability. Lignin, a byproduct of the pulp and paper industry, is a plausible material for carrying various types of cargo in small‐ and large‐scale applications. Herein, possibilities and constraints regarding the physical–chemical properties of the lignin source as well as modifications and processing required to render lignins suitable for the loading and release of pesticides, pharmaceuticals, and biological macromolecules is reviewed. In addition, the technical challenges, regulatory and toxicological aspects, and future research needed to realize some of the promises that nano‐ and microscaled lignin materials hold for a sustainable future are critically discussed.

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“…Produksi NAG dengan menggunakan kitinase yang dihasilkan oleh Mucor circinelloides tersebut cukup tinggi sehingga merupakan salah satu cara yang dapat dilakukan untuk mengurangi produksi limbah. Namun, produksi enzim cukup sulit karena prosesnya cukup lama dan cenderung tidak stabil (Zdarta et al, 2015;Prakash et al, 2018).…”

Section: Pengantarunclassified

Production of N-acetylglucosamine from Semi Purified Chitinase of Mucor circinelloides that Immobilized by using Agar

Soedirga

Hardoko

Widianto

2019

JFS

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Hydrolysis of chitin into N-acetylglucosamine (NAG) can be done enzymatically by using chitinase enzyme that obtained from the fermentation with chitinolytic molds. Mucor circinelloides is one example of chitinolytic mold that can produce semi purified chitinase enzyme during the fermentation of chitin from black tiger shrimp (Penaeus monodon) shell. The semi purified chitinase enzyme that obtained in this research will be immobilized into the agar polymer to the extent of its stability during NAG production. This research was aimed to investigated the best concentration of agar (3,4,5, and 6%) and the best amount of added enzyme (0.2; 0.4; 0.6; 0.8; and 1 mL) toward the production of NAG. The result showed 0.6 ml of semi purified chitinase that immobilized into 3% of agar can produced NAG within the concentration 1111.667 ppm. Moreover, this enzyme considerably stable before and after immobilization within the value of 4.78 U/mL.

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Chitin-Lignin Material as a Novel Matrix for Enzyme Immobilization

Cited by 80 publications

References 71 publications

Luffa cylindrica sponges as a thermally and chemically stable support for Aspergillus niger lipase

Luffa cylindrica sponges as a thermally and chemically stable support for Aspergillus niger lipase

Lignin for Nano‐ and Microscaled Carrier Systems: Applications, Trends, and Challenges

Production of N-acetylglucosamine from Semi Purified Chitinase of Mucor circinelloides that Immobilized by using Agar

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