Chitin extraction from shrimp shell waste using Bacillus bacteria

Ghorbel-Bellaaj, Olfa; Younes, Islem; Maâlej, Hana; Hajji, Sawssen; Nasri, Monçef

doi:10.1016/j.ijbiomac.2012.08.034

Cited by 121 publications

(92 citation statements)

References 45 publications

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“…This fact is also noted in the literature for shrimp waste from marine species where 98% DM and 78% DP after 10 days of fermentation with L. helveticus (Arbia et al, 2013); 91% DM and 97% DP, after 7 days of fermentation with L. acidophilus (Duan et al, 2012); DM ranging from 63 to 81% and DP of 60 the 83%, with L. plantarum (Rao & Stevens, 2005;2006). After testing six strains of Bacillus, (Ghorbel-Bellaaj et al, 2012;Ghorbel-Bellaaj et al, 2013) (Zhang et al, 2012;Zhang et al, 2014) obtained in a total time of 6 days 93% of DP and 95% of DM.…”

Section: Discussionmentioning

confidence: 99%

“…The chitin content, calculated from the total extraction of minerals and ash with acids and bases was about 35% and results obtained in our laboratory for chemical extraction of chitin ranged between 20% (Arantes et al, 2015) and 35% (current data), varying according to the filtering techniques, which can lead to loss of solid material in the washes. Works with freshwater shrimp or M. rosenbergii for comparison purposes were not found but for the marine species cited in this paper these results are comprised between the displayed values: can (Arbia et al, 2013;Duan et al, 2012;Ghorbel-Bellaaj et al, 2012;Ghorbel-Bellaaj et al, 2013;Ghorbel-Bellaaj et al, 2011). From the results obtained for ash and protein content before and after fermentation, demineralization (DM) and deproteination (DP) of carapace through fermentation with L. plantarum under different strategies were determined (Table 1).…”

Section: Shrimp Waste Characterizationmentioning

confidence: 99%

“…In the literature several papers have described the fermentation of shrimp farming residues from salt water: Use of L. helveticus was shown by Arbia, Adour, Amrane and Lounici (2013); Duan et al (2012) used L. acidophilus isolated from the residue itself; Rao and Stevens (2005;2006) used L. plantarum. In addition, other bacteria or biological methods can also be found in the literature involving the use of different strains of Bacillus (Ghorbel-Bellaaj, Younes, Maâlej, Hajji, & Nasri., 2012;Ghorbel-Bellaaj et al, 2013) and P. aeruginosa (Ghorbel-Bellaaj et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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<b>Optimization of lactic fermentation for extraction of chitin from freshwater shrimp waste

et al. 2017

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ABSTRACT. Freshwater shrimp shells from the shrimp farming activity in tanks, were processed for biological extraction of chitin, by fermentation with Lactobacillus plantarum isolated from meat products, offering an advantageous demineralization and deproteination of the residue, replacing the chemically. Deproteination was obtained approximately 99% and demineralization of up to 87% using batch fermentations with a maximum of 72 hours and the use of simple strategies such as pH adjustment and reinoculation. The performance of chitin was about 40% greater than in the chemical extraction and the results indicate an interesting method in the process of production of chitosan, where the biopolymer chitin is precursor.Keywords: shrimp shells, chitosan, Lactobacillus plantarum, bioprocess.Otimização de fermentação lática para extração de quitina de resíduos de camarão de água doce RESUMO. Carapaças de camarão de água doce provenientes da atividade de carcinicultura em tanques foram processadas para extração biológica de quitina, por meio da fermentação com Lactobacillus plantarum isolado de produtos cárneos, oferecendo uma via vantajosa de desmineralização e de desproteinização do resíduo, em substituição à via química. Obteve-se desproteinização de cerca de 99% e desmineralização de até 87% por intermédio de fermentações em batelada com duração máxima de 72 horas e o uso de estratégias simples, como ajuste de pH e reinoculação. O rendimento de quitina foi cerca de 40% maior do que na extração química, e o conjunto dos resultados indica um método interessante no processo de produção de quitosana, em que o biopolímero quitina é precursor.Palavras-chave: carapaças de camarão, quitosana, Lactobacillus plantarum, bioprocesso.

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Section: Discussionmentioning

confidence: 99%

Section: Shrimp Waste Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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<b>Optimization of lactic fermentation for extraction of chitin from freshwater shrimp waste

et al. 2017

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“…The DPPH (2,2-diphenyl-1-picrylhydrazyl) assay was carried out by following the method of Ghorbel-Bellaaj et al [19] with slight modification. Briefly, different concentrations (0.25-2.5 mg/ml) of dried deproteinized shrimp shell powder were mixed with the culture supernatant from Bacillus sp.…”

Section: Dpph Radical Scavenging Assaymentioning

confidence: 99%

“…Considering their importance, various proteolytic Bacillus spp. have been used for deproteinization process and antioxidant activity using shrimp shell as substrate [16]. Also to overcome the usage of hazardous chemicals, biological treatment offers better alternative.…”

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of Halophilic Organic Solvent Tolerant Protease from Marine Bacillus sp. APCMST-RS7 and Its Antioxidant Potentials

Maruthiah

Immanuel

Palavesam

2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Halophilic organic solvent tolerant proteolytic bacterium was isolated from the marine sediment of Rajakkamankalam estuary and identified as Bacillus sp. APCMST-RS7. The alkaline protease synthesized by Bacillus sp. APCMST-RS7 was purified by using ammonium sulphate precipitation, dialysis and DEAE-Sepharose Fast Flow column. The purified protease showed 6.60-fold purity, 26.02 U/mg specific activity with 24.30 % yield. The molecular weight of the purified alkaline protease was 32 kDa. This purified protease registered maximum activity at pH 8 and it was stable between pH 8-9 after 1.30 h of incubation. The optimum temperature registered was 50°C and it was stable between 30 and 40°C even after 1.30 h of incubation. This enzyme also showed maximum activity at 1.5 M NaCl concentration. The Km and V max values registered were, 0.0002 g/l and 1428.57 U/ml, respectively. Further, magnesium chloride, barium chloride and copper sulphate influenced this enzyme activity remarkably and it was also found to be enhanced by many of the tested surfactants and solvents. Here, the serine protease inhibitor totally inhibited the enzyme activity; hence it is of serine protease family. The candidate bacterium effectively deproteinized the shrimp shell waste with maximum protease activity. The shrimp shell hydrolysate also exhibited maximum antioxidant activity.

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