This study aimed to extract the oil from macauba pulp using a low-pressure solvent extraction, as well as characterisation of the extracts and defatted meal obtained using different solvents (n-hexane, ethyl acetate and isopropanol
The study of the ecology of fermented sausage is fundamental to understand the physical and chemical changes that happen during fermentation and maturation. The aim of the present study was to determine the microbiological characteristics of sausages produced by spontaneous fermentation. Fifty samples of sausages produced in the South of Brazil by different small manufacturers were analyzed for the following microbiota: aerobic mesophilic bacteria; Micrococcaceae; mold and yeast; lactic acid bacteria; total and fecal coliforms; coagulase-positive Staphylococcus, and Salmonella. In most samples (72%), the count of lactic bacteria was higher than 6 log10 cfu.g-1, and the samples with the highest counts were above 8 log10 cfu.g-1. The counts of Micrococcaceae in most samples were between 5 log10 and 7 log10 cfu.g-1. With respect to the presence of molds and yeasts, there was a significant variation among the samples with counts ranging from 2 log10 cfu.g-1 and 6 log10 cfu.g-1. From the data obtained, it was possible to conclude that 24% of the analyzed samples did not comply with the current law in Brazil since the levels of fecal coliforms or coagulase-positive Staphylococcus exceeded the maximum limit allowed.
The most promising microorganisms for use as starter cultures are those isolated from the native microbiota of traditional fermented products. The aim of this study was to evaluate the use of lactic acid bacteria selected from the native microbiota of sausages produced by spontaneous fermentation as starter cultures for the production of sausage. Strains of Lactobacillus plantarum 503 and 341 have the potential for use as starter cultures in the manufacture of Italian sausage type. The population of lactic acid bacteria in sausages was >8 log CFU.g −1 during fermentation, which caused the pH to decrease to <4.5. This decrease in pH and the water activity of < 0.90 of sausages ensures the safety and preservation of this product. Sausages produced with these lactic cultures fulfill the requirements for microbiological quality and composition of Italian sausage type. Our results suggest the possibility of using these starter cultures for the production of sausages with peculiar characteristics that contribute to the identity of the product.Keywords: lactic acid bacteria; Lactobacillus; embedded fermented meat; sausage.Practical Application: New autochthonous starter cultures contributes to the production of high quality sausage.
ABSTRACT. Freshwater shrimp shells from the shrimp farming activity in tanks, were processed for biological extraction of chitin, by fermentation with Lactobacillus plantarum isolated from meat products, offering an advantageous demineralization and deproteination of the residue, replacing the chemically. Deproteination was obtained approximately 99% and demineralization of up to 87% using batch fermentations with a maximum of 72 hours and the use of simple strategies such as pH adjustment and reinoculation. The performance of chitin was about 40% greater than in the chemical extraction and the results indicate an interesting method in the process of production of chitosan, where the biopolymer chitin is precursor.Keywords: shrimp shells, chitosan, Lactobacillus plantarum, bioprocess.Otimização de fermentação lática para extração de quitina de resíduos de camarão de água doce RESUMO. Carapaças de camarão de água doce provenientes da atividade de carcinicultura em tanques foram processadas para extração biológica de quitina, por meio da fermentação com Lactobacillus plantarum isolado de produtos cárneos, oferecendo uma via vantajosa de desmineralização e de desproteinização do resíduo, em substituição à via química. Obteve-se desproteinização de cerca de 99% e desmineralização de até 87% por intermédio de fermentações em batelada com duração máxima de 72 horas e o uso de estratégias simples, como ajuste de pH e reinoculação. O rendimento de quitina foi cerca de 40% maior do que na extração química, e o conjunto dos resultados indica um método interessante no processo de produção de quitosana, em que o biopolímero quitina é precursor.Palavras-chave: carapaças de camarão, quitosana, Lactobacillus plantarum, bioprocesso.
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