Chitin assay to estimate the growth of<i>Candida albicans</i>in organs of infected mice

Rementerı́a, Aitor; Ezkurra, Pilar Ariadna; Hernando, Fernando; Pontón, José; Sevilla, Marı́a Jesús; Cisterna, R

doi:10.1080/02681219180000041

Cited by 13 publications

(7 citation statements)

References 9 publications

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“…First, samples were hydrolyzed with 6 M HCl at 100°C for 2 h and adjusted to pH 10.0 after cooling down. Then, hydrolyzed samples (250 µL) were treated as described by Rementeria et al (1991) . Samples absorbance was measured at 530 nm using an Evolution 600 UV‐vis (Thermo Fisher Scientific, Spain) spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Morales

Piris

Ruiz-Rodrı́guez

et al. 2018

Biotechnology Progress

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Three extraction methods were sequentially combined to obtain fractions from Lentinula edodes (shiitake mushrooms) containing bioactive compounds against cardiovascular diseases (CVDs). Fruiting bodies were first extracted with plain water, obtained residue was then submitted to supercritical fluid extraction (SFE) and remaining residue submitted to hot water extraction. Sequential design allowed reutilization of the nonextracted material as raw material for the successive extractions increasing extraction yields and separating interesting compounds. Obtained fractions contained different amounts of ß-glucans, chitins, eritadenine, lenthionine, ergosterol, proteins/peptides and phenolic compounds conferring them different bioactivities. Water soluble fractions showed high antioxidant activities (ABTS and DPPH scavenging capacity and reducing power), they were also able to inhibit one of the main enzymes involved in hypertension (angiotensin-I converting enzyme) and the key enzyme of cholesterol metabolism (3-hydroxy-3-methylglutaryl coenzyme A reductase). The latter inhibitory activity was also noticed in SFE extracts although ergosterol and other lipid-like molecules were isolated. Dietary fibers were separated in the third extraction. Therefore, with this sequential extraction procedure bioactive compounds against CVDs can be selectively separated from a single batch of shiitake powder. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:746-755, 2018.

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Section: Methodsmentioning

confidence: 99%

Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Morales

Piris

Ruiz-Rodrı́guez

et al. 2018

Biotechnology Progress

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show abstract

“…Briefly, precipitated extracts were hydrolyzed with 6 M HCl at 100 o C for 2 h. After the hydrolysis, samples were cooled down and adjusted to pH 10.0. Then, hydrolyzed samples (250 µL) were treated as described by Rementeria et al (1991) and absorbance was measured at 530 nm. A glucosamine hydrochloride standard curve was used for quantification.…”

Section: Determination Of Carbohydratesmentioning

confidence: 99%

Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes

Morales

Smiderle

Villalva

et al. 2019

Journal of Functional Foods

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“…C. albicans E‐139 was maintained on GYE‐agar (glucose, 20 g l −1 ; yeast extract, 10 g l −1 ; agar 20 g l −1 ). The inoculum for the experimental infection of the animals was prepared as previously described [20]. Briefly, yeast cells (1×10 7 cells ml −1 ) grown in medium 199 (Sigma) at 24°C for 24 h were transferred to fresh medium 199.…”

Section: Methodsmentioning

confidence: 99%

“…The chitin concentration in organs from infected mice was quantified as previously described [20]. Briefly, the organs were successively extracted with 3% sodium lauryl sulfate at 100°C, 0.12% KOH at 130°C and 75% ethanol plus 0.12% KOH at 0°C.…”

Section: Methodsmentioning

confidence: 99%

Resistance to candidiasis and macrophage activity in chitin-treated mice

Rementerı́a

Abaitua

García-Tobalina

et al. 2006

FEMS Immunology &Amp; Medical Microbiology

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The effect of chitin, a polysaccharide of the cell wall of Candida albicans, on both the survival of C. albicans infected mice and the activity of the murine peritoneal macrophages has been studied. Pretreatment of mice with 30 mg kg(-1) C. albicans chitin enhanced the survival of the infected animals. The protective effect was concomitant with an enhancement of both phagocytic and candidacidal activities of the peritoneal macrophages. Chitin by itself did not induce the nitric oxide (NO) synthase in the macrophages, which remained at a level similar to that shown by the macrophages from untreated animals. The administration of 10 mg kg(-1) C. albicans chitin diminished the long term survival of the infected animals. This effect was coincident with a lower candidacidal activity and NO production by the macrophages of the chitin treated and infected animals, compared to the untreated infected animals.

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Chitin assay to estimate the growth ofCandida albicansin organs of infected mice

Cited by 13 publications

References 9 publications

Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes

Resistance to candidiasis and macrophage activity in chitin-treated mice

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