Chitin and chitosan-based support materials for enzyme immobilization and biotechnological applications

Verma, Madan L.; Kumar, Sandeep; Das, Anamika; Randhawa, Jatinder S.; Chamundeeswari, M.

doi:10.1007/s10311-019-00942-5

Cited by 168 publications

(79 citation statements)

References 91 publications

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“…In recent decades, immobilization techniques have been successfully applied to enhance enzyme properties, in particular stability and reusability [ 2 ]. The material employed for the support preparation is of fundamental importance in the immobilization process, affecting the catalytic properties of the produced biocatalyst.…”

Section: Introductionmentioning

confidence: 99%

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Papain Covalently Immobilized on Chitosan–Clay Nanocomposite Films: Application in Synthetic and Real White Wine

et al. 2020

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Increasing attention has been recently paid to the development of nanocomposite materials for food application as new tool to enhance the mechanical and thermal properties of polymers. In this study, novel chitosan–clay nanocomposite films were produced as carriers for the covalent immobilization of papain, by using a fixed amount of chitosan (1% w/v) and a food-grade activated montmorillonite (Optigel, OPT) or a high-purity unmodified montmorillonite (SMP), in four different weight percentages with respect to chitosan (i.e., 20, 30, 50, 70% w/w). Both nanoclays (OPT and SMP) improved the mechanical properties of the obtained nanocomposites, and the OPT films showed the highest Young modulus and mechanical resistance (σmax). The nanocomposites were used as carriers for the covalent immobilization of papain, which was preliminarily characterized in model wine towards a synthetic substrate, showing the highest efficiency in the release of the reaction product when it was bound on OPT-30 and OPT-50 films. Finally, the latter biocatalyst (papain on OPT-50 film) was applied for the protein stabilization of two different unfined white wines, and it efficiently reduced both the haze potential and the protein content.

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Section: Introductionmentioning

confidence: 99%

“…Among biopolymers, chitosan (CS, a linear polysaccharide made of N -acetyl- d -glucosamine and d -glucosamine units [ 11 ]) is the most commonly used for the production of immobilization supports as it is, as well as in composite/nanocomposite forms [ 2 ], to be applied in the food and pharmaceutical industries.…”

Section: Introductionmentioning

confidence: 99%

Papain Covalently Immobilized on Chitosan–Clay Nanocomposite Films: Application in Synthetic and Real White Wine

et al. 2020

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“…As the particle creates bonding with the enzyme, its structure and properties have a significant influence on the enzymatic activity of the immobilized catalyst. Both organic polymers (cellulose, starch, chitin, chitosan, silica alginate) and chemically synthesized inorganic molecules are used in the immobilization process (Al-Adhami et al 2002 ; Wang et al 2011 ; Kampmann et al 2014 ; Verma et al 2020 ). The organic ones, owing to their natural source, exhibit enhanced biological compatibility toward the enzyme.…”

Section: Potential Of Wrf To Remove Bpa Bps and Np From Wastewatermentioning

confidence: 99%

White rot fungi can be a promising tool for removal of bisphenol A, bisphenol S, and nonylphenol from wastewater

Grelska

Noszczyńska

2020

Environ Sci Pollut Res

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Endocrine-disrupting chemicals (EDC) are a wide group of chemicals that interfere with the endocrine system. Their similarity to natural steroid hormones makes them able to attach to hormone receptors, thereby causing unfavorable health effects. Among EDC, bisphenol A (BPA), bisphenol S (BPS), and nonylphenol (NP) seem to be particularly harmful. As the industry is experiencing rapid expansion, BPA, BPS, and NP are being produced in growing amounts, generating considerable environmental pollution. White rot fungi (WRF) are an economical, ecologically friendly, and socially acceptable way to remove EDC contamination from ecosystems. WRF secrete extracellular ligninolytic enzymes such as laccase, manganese peroxidase, lignin peroxidase, and versatile peroxidase, involved in lignin deterioration. Owing to the broad substrate specificity of these enzymes, they are able to remove numerous xenobiotics, including EDC. Therefore, WRF seem to be a promising tool in the abovementioned EDC elimination during wastewater treatment processes. Here, we review WRF application for this EDC removal from wastewater and indicate several strengths and limitations of such methods.

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“…Natural and synthetic polymers such as chitosan, alginate, gelatin, agarose (some of them in the form of Sepharose), polyvinyl alcohol, polyethyleneimine, polyester, polyacrylamide, and some resins, such as DEAE-cellulose and Duolite have also been studied as supporting material for the immobilization of b-galactosidase (Cao 2005;Verma, Kumar, et al 2020). Li et al (2008) reported the production of GOS with b-galactosidase immobilized in calcium alginate.…”

Section: Support Materials and Approaches For Enzymatic Production Ofmentioning

confidence: 99%

Recent advances in β-galactosidase and fructosyltransferase immobilization technology

Ureta

Martins

Figueira

et al. 2020

Critical Reviews in Food Science and Nutrition

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The highly demanding conditions of industrial processes may lower the stability and affect the activity of enzymes used as biocatalysts. Enzyme immobilization emerged as an approach to promote stabilization and easy removal of enzymes for their reusability. The aim of this review is to go through the principal immobilization strategies addressed to achieve optimal industrial processes with special care on those reported for two types of enzymes: b-galactosidases and fructosyltransferases. The main methods used to immobilize these two enzymes are adsorption, entrapment, covalent coupling and cross-linking or aggregation (no support is used), all of them having pros and cons. Regarding the support, it should be cost-effective, assure the reusability and an easy recovery of the enzyme, increasing its stability and durability. The discussion provided showed that the type of enzyme, its origin, its purity, together with the type of immobilization method and the support will affect the performance during the enzymatic synthesis. Enzymes' immobilization involves interdisciplinary knowledge including enzymology, nanotechnology, molecular dynamics, cellular physiology and process design. The increasing availability of facilities has opened a variety of possibilities to define strategies to optimize the activity and re-usability of b-galactosidases and fructosyltransferases, but there is still great place for innovative developments.

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Chitin and chitosan-based support materials for enzyme immobilization and biotechnological applications

Cited by 168 publications

References 91 publications

Papain Covalently Immobilized on Chitosan–Clay Nanocomposite Films: Application in Synthetic and Real White Wine

Papain Covalently Immobilized on Chitosan–Clay Nanocomposite Films: Application in Synthetic and Real White Wine

White rot fungi can be a promising tool for removal of bisphenol A, bisphenol S, and nonylphenol from wastewater

Recent advances in β-galactosidase and fructosyltransferase immobilization technology

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