Chiral separations af atropine and homatropine on an α1-acid glycoprotein-bonded stationary phase

Arvidsson, E.; Jansson, Sven-Olof; Schill, G

doi:10.1016/s0021-9673(01)91606-1

Cited by 26 publications

(9 citation statements)

References 10 publications

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“…The same effect was obtained by using albumin as the immobilised protein [8]. However, the most common effect by using protein phases is that an increased column temperature resulted in decreased separation factors [10]. In the present study, varying the column temperature gave a large contribution to the enantioselectivity.…”

Section: Modelled Enan Tioselectivitysupporting

confidence: 73%

“…From previous experimental data it was observed that the enantioselectivity of amines could be affected by changing polar additives e.g. buffer components in the mobile phase [10]. The absence of effects from the ionic strength presented in this study, was probably caused by the necessity to use a rather high content of uncharged modifiers in the mobile phases compared to the content used in previous investigations.…”

Section: Modelled Enan Tioselectivitymentioning

confidence: 46%

“…The effect of the column temperature on enantioselective retention has earlier been studied using immobilised proteins as chiral selectors [8,10,26]. The most common effect is that the capacity factors decrease by increasing the column temperature [10].…”

Section: Modelled Capacity Factors (K')mentioning

confidence: 99%

“…The most common effect is that the capacity factors decrease by increasing the column temperature [10]. However, by using cellulase as the immobilised protein and racemates of 13-receptor blocking agents as solutes, as well as using immobilised albumin and racemic tryptophane as solute, increased capacity factors were obtained with increasing column temperature [8,26].…”

Section: Modelled Capacity Factors (K')mentioning

confidence: 99%

See 3 more Smart Citations

Optimisation of chiral separation of omeprazole and one of its metabolites on immobilized α1-acid glycoprotein using chemometricsglycoprotein using chemometrics

Hermansson

1997

Chromatographia

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SummaryA strategy for the optimisation of direct chiral separation of omeprazole and a metabolite, hydroxiomeprazole, in reversed phase liquid chromatography is described. A factorial design was used, where mobile phase pH, concentration of a mobile phase modifier, ionic strength and column temperature were tested as the variables and enantioselective retention, column efficiency and asymmetry factor as the responses. The experimental results were evaluated with multivariate analyses, which demonstrated that the column temperature and content of mobile phase acetonitrile were by far the most important variables. The enantiomers of omeprazole and one of its metabolites were baseline resolved within 15 minutes. The optimised chromatographic system was used for a separation of the enantiomers of omeprazole and its main metabolite in a patient plasma sample.

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Section: Modelled Enan Tioselectivitysupporting

confidence: 73%

Section: Modelled Enan Tioselectivitymentioning

confidence: 46%

Section: Modelled Capacity Factors (K')mentioning

confidence: 99%

Section: Modelled Capacity Factors (K')mentioning

confidence: 99%

See 2 more Smart Citations

Optimisation of chiral separation of omeprazole and one of its metabolites on immobilized α1-acid glycoprotein using chemometricsglycoprotein using chemometrics

Hermansson

1997

Chromatographia

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show abstract

“…Hyosc has been enantioselectively resolved on the ␣ 1 -acid glycoprotein-CSP [29,30], on the human serum albumine-triazine CSP [31] and the teicoplanin-based Chirobiotic T CSP [32]. The chromatographic separation and determination of tropane and related alkaloids has been recently reviewed by Dräger [33].…”

Section: Introductionmentioning

confidence: 99%

HPLC–atmospheric pressure chemical ionization mass spectrometric method for enantioselective determination of R,S-propranolol and R,S-hyoscyamine in human plasma

Siluk¹,

Mager²,

Gronich³

et al. 2007

Journal of Chromatography B

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Chiral resolution of atropine, homatropine and eight synthetic tropinyl and piperidinyl esters by capillary zone electrophoresis with cyclodextrin additives

Jin

Yan

et al. 1999

Electrophoresis

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Chiral separations af atropine and homatropine on an α1-acid glycoprotein-bonded stationary phase

Cited by 26 publications

References 10 publications

Optimisation of chiral separation of omeprazole and one of its metabolites on immobilized α1-acid glycoprotein using chemometricsglycoprotein using chemometrics

Optimisation of chiral separation of omeprazole and one of its metabolites on immobilized α1-acid glycoprotein using chemometricsglycoprotein using chemometrics

HPLC–atmospheric pressure chemical ionization mass spectrometric method for enantioselective determination of R,S-propranolol and R,S-hyoscyamine in human plasma

Chiral resolution of atropine, homatropine and eight synthetic tropinyl and piperidinyl esters by capillary zone electrophoresis with cyclodextrin additives

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