Chiral separation of cetirizine by capillary electrophoresis

Eeckhaut, Ann Van; Michotte, Yvette

doi:10.1002/elps.200500844

Cited by 32 publications

(24 citation statements)

References 45 publications

(31 reference statements)

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“…[17]. Eeckhaut et al established optimized conditions for enantioseparation of cetirizine including heptakis (2,3-diacetyl-6-sulfato)-b-CD as chiral selector, organic modifier, and co-ion in phosphate buffer (pH 2.5) [18]. We attempted to simplify the separation conditions; therefore, the enantioseparation of cetirizine by simple CD-mediated CE was studied.…”

Section: Resultsmentioning

confidence: 99%

“…The sulfated groups in b-CD provide a derivative which is anionic over the entire pH range accessible to CE experiments and which can thereby lead to ionic interactions in addition to hydrophobic inclusion. The ionizable functional groups in the structure of cetirizine have an acidic group (pK a 2.9) and two basic groups (pK a 2.2 and 8.0) [18]. Hence, its enantiomers exist almost exclusively as a zwitterion in the pH region 3.5 -7.5 [3].…”

Section: Concentration and Ph Of Borate Buffer Systemmentioning

confidence: 98%

“…The detection limits (LOD) of cetirizine enantiomers are 5.27 and 5.34 lg/mL, respectively; and the limits of quantification (LOQ) of cetirizine enantiomers are 17.57 and 17.80 lg/mL, respectively. Eeckhaut et al [18] reported the CE separation of cetirizine enantiomers with phosphate buffer at pH 2.5 using heptakis(2,3-diacetyl-6-sulfato)-b-CD, triethanolamine, and acetonitrile as chiral selector, co-ion, and organic modifier, respectively. The LOD and LOQ are approximately three times better than those obtained by MikuÐ et al [17].…”

Section: Introductionmentioning

confidence: 99%

“…Background electrolytes with cyclodextrins (CDs) and their derivatives are most frequently used as chiral selectors. Recently, some CE methods have been used to investigate the enantioseparation of cetirizine [17,18]. MikuÐ et al [17] described a CE enantioseparation of cetirizine racemate, using dynamic coating of capillary with methylhydroxylethylcellulose in dual b-CDs and 25 mM morpholinoethanesulfonic acid (pH 5.2) as chiral selector and buffer solution.…”

Section: Introductionmentioning

confidence: 99%

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Enantioseparation of cetirizine by sulfated‐β‐cyclodextrin‐mediated capillary electrophoresis

Chou

Huang

et al. 2008

J of Separation Science

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A sulfated beta-cyclodextrin (sulfated beta-CD)-mediated capillary electrophoresis method is described for the enantioseparation of cetirizine using achiral cefazolin as an internal standard. The enantioseparation of the drug was performed in a borate buffer (5 mM, pH 8.7) with 1% sulfated beta-CD (w/v) as chiral selector at 10 kV. Several parameters affecting the separation were studied, including the pH and the concentration of borate buffer and chiral selector. Under optimized conditions, a baseline separation of two enantiomers was achieved in less than 7 min. Using cefazolin as an internal standard (IS), the linear range of the method for the determination of levocetirizine was over 1.0 to 50.0 microg/mL; the detection limit (signal-to-noise ratio = 3) of levocetirizine was 0.5 microg/mL. The method allowed the enantioseparation of cetirizine in bulk samples and enantiomeric purity evaluation of levocetirizine (R-enantiomer) in pharmaceutical tablets (Xyzal), and it was also found to be suitable for enantioseparation in human plasma.

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Section: Resultsmentioning

confidence: 99%

Section: Concentration and Ph Of Borate Buffer Systemmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enantioseparation of cetirizine by sulfated‐β‐cyclodextrin‐mediated capillary electrophoresis

Chou

Huang

et al. 2008

J of Separation Science

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“…UV (ultraviolet) detection was performed for the quantification of levocetirizine in the tablets and for enantiomeric purity testing of the drug by a validated, selective, precise and accurate method [8].…”

mentioning

confidence: 99%

Drug-Drug Interaction Studies of Levocetirizine with Atenolol

Mehboob¹,

Mughal²,

Aftab³

et al. 2017

JPP

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Abstract:The objective of the study was to evaluate the drug-drug interaction studies of levoceterizine with atenolol. Calibration curve studies of working standard solutions of levocetirizine and atenolol (0.01~0.1 mmol) were scanned. Maxima appeared at 231 nm for levocetirizine and 224 nm for atenolol. The calibration curve obeyed Beer Lambert's Law. Lone availabilities of both the drugs were studied in pH 1, pH 4, pH 7.4 and pH 9 at 37 °C on B.P. (British Pharmacopoeia) dissolution apparatus. To study the drug-drug interaction of levocetirizine (5 mg tablet) and atenolol (100 mg tablet), both the drugs were introduced to the dissolution apparatus in simulated gastric juice (pH 1), pH 4, pH 7.4 and pH 9 at 37 °C at zero time and measured the absorbance maxima of both the drugs at the corresponding wavelength. Graphs were plotted for availability percentage (%) of drug versus time at each set of experiment. The availability percentage (%) of levocetirizine in the buffers of pH simulated to gastric pH 4, pH 7.4 and pH 9 in the presence of atenolol was 436.78%, 376.90%, 436.78% and 436.78%, respectively, but the availability of atenolol was increased up to 214.80%, 212.96%, 214.93% and 231.51% in simulated to gastric pH and in the buffers of pH 4, pH 7.4 and pH 9, respectively. On the basis of these studies, it is concluded that levocetirizine forms a charge-complex with atenolol; therefore, co-administration of these drugs should be avoided.

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Comparison of HPLC and CE methods for the determination of cetirizine dihydrochloride in human plasma samples

Kowalski

Plenis

2007

Biomedical Chromatography

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Two methods, capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC), for analysis of cetirizine dihydrochloride in small sample volumes of human plasma were compared. The CE and HPLC assays were developed and validated by analyzing a series of plasma samples containing cetirizine dihydrochloride in different concentrations using these two methods. The extraction procedure is simple and no complicated purification steps or derivatization are required. The analysis time in the HPLC method was shorter than that in the CE method, but solvent consumption was considerably lower in the CE method. The calibration curve was linear to at least 10-1000 ng/mL both for CE and HPLC with r(2) = 0.9993 and r(2) = 0.9994, respectively. The detection limits for cetirizine dihydrochloride were 3 and 5 ng/mL with CE and HPLC (a UV detector was applied in the both cases), respectively. Both methods were selective, robust and specific, allowing reliable quantification of cetirizine dihydrochloride, and could be useful for clinical and biomedical investigations.

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Chiral separation of cetirizine by capillary electrophoresis

Cited by 32 publications

References 45 publications

Enantioseparation of cetirizine by sulfated‐β‐cyclodextrin‐mediated capillary electrophoresis

Enantioseparation of cetirizine by sulfated‐β‐cyclodextrin‐mediated capillary electrophoresis

Drug-Drug Interaction Studies of Levocetirizine with Atenolol

Comparison of HPLC and CE methods for the determination of cetirizine dihydrochloride in human plasma samples

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