Chiral separation of acidic drug components by open tubular electrochromatography using avidin immobilized capillaries

Kitagawa, Fumihiko; Inoue, Keisuke; Hasegawa, Takayuki; Kamiya, M.; Okamoto, Yukihiro; Kawase, Masaya; Otsuka, Koji

doi:10.1016/j.chroma.2006.03.077

Cited by 50 publications

(44 citation statements)

References 51 publications

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“…Moreover, most of the coupling methods applied are built-up or developed in-house and experience in the fabrication and handling is needed [36]. As has been also reviewed by Klampfl [4], developments of the interface were reported [36,37], although more papers address better solutions for the capillary fabrication such as the use of novel separation materials [38][39][40][41], improvements of the packing method [42] or alternations of the separation capillary [43].…”

Section: Trends In Cec-msmentioning

confidence: 96%

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Trends in CE‐MS 2005–2006

et al. 2007

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CE-MS has gained further attention as a multidimensional analytical approach. The number of publications dealing with this technique is still increasing on the level of application as well as method development-oriented approaches. Additionally, 15 reviews were published the last two years focusing on CE-MS. An overview of all papers found to have been published within 2005 and 2006 is given in tabulated form. Additionally, four promising technical trends are chosen to be presented in detail: (i) chip-based CE-MS, (ii) capillary coatings in CE-MS, (iii) new trends in CEC-MS and (iv) the application of 2-D CE-MS.

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Section: Trends In Cec-msmentioning

confidence: 96%

“…4 [45]. Another application was published recently based on the chemical immobilization of avidin onto the fused-silica surface, which provided a possibility to separate acidic drug components in affinity mode (ACEC) [39].…”

Section: Trends In Cec-msmentioning

confidence: 99%

Trends in CE‐MS 2005–2006

et al. 2007

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“…A good example is the use of covalently immobilized avidin on the capillary surface to produce a stable coating with chiral recognition and MS compatibility (19). Avidin-modified coatings showed good chiral recognition for abscisic acid enantiomers and lasted 50 days with over 100 runs.…”

Section: Capillary Coatings and Surface Modificationsmentioning

confidence: 99%

Capillary Electrophoresis in Bioanalysis

2008

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Science reports ∼4800 hits on capillary electrophoresis (CE) including 410 reviews. Because of the prominence of CE techniques in bioanalysis, we decided make them the focus of this review and selected 200 hundred papers representing advances in this field. The selected papers cover advances in CE theory, instrumentation, and methodologies that are specific to various analytes of biological origin or relevance. The group of analytes includes nucleic acids, proteins and peptides, carbohydrates, lipids, single cells, and bioparticles. In addition, we have included advances in the use of CE to define functional assays or to investigate biomolecular interactions. The use of microfabricated devices for CE analysis was not included because this is already covered in other review. Technique Developments Separation SchemesDetermining the velocity of the electroosmotic flow (EOF) and how it changes during an electrophoretic separation is still an important research topic. A simple method for EOF measurements using so-called thermal marks was reported (1). Here, a tungsten filament caused punctual heating at the capillary wall and caused a perturbation in the electrolyte concentration. A sequence of these "thermal marks" then migrated with the EOF until each mark reached and was detected by a conductivity detector. The feasibility of using thermal marks as internal EOF standards in different separation systems was thereby demonstrated.Isoelectric focusing separates amphoteric analytes such as proteins or peptides by the differences in their isoelectric points. Most of the reports on capillary isoelectric focusing (cIEF) describe an initial focusing phase after which the focused zones are mobilized and detected. A dynamic cIEF method for protein analysis was reported (2). This technique made it possible to control each protein's position and focused width by moving the pH gradient within the capillary through manipulation of the electric fields. An important advantage of this approach is the capability of collecting focused analytes from the central section, suggesting that there may be great potential for introducing selectively focused proteins to a second separation dimension such as LC or CE.Micellar electrokinetic capillary chromatography (MEKC) is typically incompatible with electrospray mass spectrometry (ESI-MS) because the nonvolatile surfactants in the micellar phase result in complicated adduct formation and loss of sensitivity during the electrospray process and because the presence of the organic solvent needed for electrospray may cause instability in the micellar phase. These drawbacks were overcome by using synthetic polymeric surfactants that can work as a pseudostationary phase and provide stable electrospray (3). The polymeric surfactant was made by polymerizing three amino acidderived (L-leucinol, L-isoleucinol, L-valinol) sulfated chiral surfactants. These polymeric

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“…Kitagawa et al [86] immobilized an avidin selector in OT CEC to produce more stable columns compared with the classically coated column. Run-to-run repeatability for migration time had an RSD of 0.8%, while interday repeatability for Rs was below 8.9%.…”

Section: Applications In Ot Cecmentioning

confidence: 99%

Enantioselective capillary electrochromatography: Recent developments and new trends

Mangelings

Heyden

2011

Electrophoresis

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Since its development in the early 1970s, CEC has been studied quite extensively, but unfortunately its use is still mostly located at an academic level. Reasons for this are the limited availability of commercially available stationary phases (SPs) and columns, along with some practical limitations, such as column fragility, lack of column robustness and reproducibility. Nevertheless, CEC maintains a place among the separation techniques, probably because of its unique feature to combine two separation principles. Also in the field of chiral separations, CEC is often used as a separation technique and already showed its potential for this kind of analyses. This overview will focus on the recent applications, i.e. between 2006 and 2010, in enantioselective analysis by means of CEC. For the selected applications, the used SPs (chiral selectors) and their potential for future method development or screening purposes will be evaluated and critically discussed.

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Chiral separation of acidic drug components by open tubular electrochromatography using avidin immobilized capillaries

Cited by 50 publications

References 51 publications

Trends in CE‐MS 2005–2006

Trends in CE‐MS 2005–2006

Capillary Electrophoresis in Bioanalysis

Enantioselective capillary electrochromatography: Recent developments and new trends

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