Chiral ligand-exchange chromatography for diastereo-enantio separation of exametazime

HPLC enantioseparation of racemic bupropion, baclofen and etodolac: modification of conventional ligand exchange approach by pre‐column formation of chiral ligand exchange complexes

“…The principle and application of chiral ligand exchange chromatography have been well documented in the literature (Davankov and Rogozhin, ; Sanaie and Haynes, 2006 , ; Natalini et al ., ) and the technique has been found to be successful for direct enantiomeric (HPLC) resolution of amino acids, β‐blockers and hydroxy acids by HPLC (Yamazaki et al ., ; Bazylak, ; Katoh et al ., ; Nikolic et al ., ; Koidl et al ., ; Sun et al ., ; Sotgia et al ., ; Kumar and Bhushan, ).…”

“…In all there were 12 pairs of diastereomeric ligand exchanged complexes; their UV spectra were recorded to verify the formation of such diastereomeric complexes prior to HPLC analysis. The principle and application of chiral ligand exchange chromatography have been well documented in the literature (Davankov and Rogozhin, 1971;Sanaie and Haynes, 20062006a, 2006bNatalini et al, 2008Natalini et al, , 2010 and the technique has been found to be successful for direct enantiomeric (HPLC) resolution of amino acids, β-blockers and hydroxy acids by HPLC (Yamazaki et al, 1994;Bazylak, 1994;Katoh et al, 1989;Nikolic et al, 2003;Koidl et al, 2008;Sun et al, 2006;Sotgia et al, 2008;Kumar and Bhushan, 2014).…”

O-Benzyl-(S)-Serine, a New Chiral Selector for Ligand-Exchange Chromatography of Amino Acids

“…Mechanism of Chiral Separation Racemic His (3), Pro (2) and Phg (1) as test compounds have been submitted to CMP elution with different Cu(II) concentrations ( Table 3). By maintaining constant both pH value (4.00) and chiral selector/Cu(II) ion concentration ratio (2:1), an increase in copper(II) ion concentration from 0.25 mM to 0.87 mM results in a strongly reduced retention A previous, detailed work [23] pointed out the non linear relationship between the lipophilic character of the selector and k'.…”

“…Among the chromatographic techniques aimed at resolving underivatized amino acid enantiomers, chiral ligand-exchange chromatography (CLEC) still constitutes one of the most employed methodologies [1] as documented in recent research performed in both chiral mobile phase (CMP) mode [2][3][4] and chiral stationary phase (CSP) approach, splitted in covalently bound [5,6] to a solid support or dynamically, hydrophobically bound [7,8] to a sorbent surface. In the past few decades, the field of chiral recognition and separation of chiral compounds has received considerable attention.…”

Enantioseparation of atenolol using chiral ligand-exchange chromatography on C8 column

“…This has highlighted the need for enantioselective separation of chiral drugs. For many racemic drugs, the desired pharmacologic effect is considerably due to one enantiomer, while its antipode may be responsible for undesirable side-effects [1][2][3][4][5][6][7].…”