Chiral analysis of methorphan in opiate-overdose related deaths by using capillary electrophoresis

Bertaso, Anna; Musile, Giacomo; Gottardo, Rossella; Seri, Catia; Tagliaro, Franco

doi:10.1016/j.jchromb.2015.07.024

Cited by 19 publications

(16 citation statements)

References 21 publications

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“…This will, in turn, make it more suitable for applications. HP‐β‐CD was also used for the enantioselective determination of methorphan and its main metabolites, dextrophan, and levorphanol . The optimum method was successfully applied to the analysis of postmortem blood samples obtained from ten subjects who died from heroin overdoses.…”

Section: Chiral Selectorsmentioning

confidence: 99%

Chiral selectors in CE: Recent development and applications (mid‐2014 to mid‐2016)

Stavrou

Agathokleous

Kapnissi‐Christodoulou

2017

Electrophoresis

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This report, which is a sequence of a series of reviews, records the most important chiral selectors (CSs) applied in CE. It highlights the CSs that were used during the period 2014 to mid-2016. In this review, method developments, validations, and pharmaceutical along with biomedical applications are presented. The different CSs include CDs, antibiotics, cyclofructants, linear and branched oligo- and polysaccharides, and polymeric surfactants. In addition, the advantages of these CSs, along with their chiral recognition mechanisms, and their performance, are discussed.

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Section: Chiral Selectorsmentioning

confidence: 99%

Chiral selectors in CE: Recent development and applications (mid‐2014 to mid‐2016)

Stavrou

Agathokleous

Kapnissi‐Christodoulou

2017

Electrophoresis

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“…Under these conditions, LODs of 0.9 parts per million (ppm) were achieved. LLE has also been used in the extraction of ketamine and its metabolites from hair or biological fluids , methorphan and its metabolites from blood , propranolol from biological fluids , and methamphetamine from hair . These last works, in addition to using LLE, also employ an in‐capillary sample preconcentration technique to improve the sensitivity of the chiral separations obtained (see Table ) and are described in Section .…”

Section: Strategies To Improve the Detection Sensitivitymentioning

confidence: 99%

“…Another EKC methodology using FASI conditions was described for the determination of methorphan and its main metabolites extracted previously with LLE of post‐mortem blood samples from subjects died for heroin overdoses . The LOD using UV detection at 260 nm was only 8 ppb.…”

Section: Strategies To Improve the Detection Sensitivitymentioning

confidence: 99%

Recent contributions for improving sensitivity in chiral CE

2017

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The flexibility and versatility of the chiral CE are unrivaled and the same instrumentation can be used to separate a diverse range of analytes, both large and small molecules, whether charged or uncharged. However, one of the disadvantages is generally thought to be the poor sensitivity of ultraviolet (UV) detection, which is the most popular among CE detectors. This review focuses on methodologies and applications regarding improvements of sensitivity in chiral CE published in the last 2 years (June 2015 until May 2017). This contribution continues to update this series of biannual reviews, first published in Electrophoresis in 2006. The main body of the review brings a survey of publications organized according to different approaches to detect a low amount of analytes, either by sample treatment procedures or by in-capillary sample preconcentration techniques, both using UV detection, or even by employing detection systems more sensitive than UV absorption, such as LIF or MS. This review provides comprehensive tables listing the new approaches in sensitive chiral CE with categorizing by the fundamental mechanism to enhance the sensitivity, which provide relevant information on the strategies employed. The concluding remarks in the final part of the review evaluate present state of art and the trends for sensitivity enhancement in chiral CE.

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“…An enantioselective determination of methorphan and its main metabolites in blood was described by Bertaso et al. . Enantiomeric separations were carried out with a BGE composed of 150 mM sodium phosphate at pH 4.4 with 5 mM 2‐(hydroxypropyl)‐β‐CD (HP‐β‐CD) and 20% methanol (MeOH).…”

Section: Concentration Adjustment (Kohlrausch Stacking)mentioning

confidence: 99%

Recent progress of sample stacking in capillary electrophoresis (2014–2016)

et al. 2016

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The term "sample stacking" comprises a relatively broad spectrum of techniques that already form an almost inherent part of the methodology of CZE. Their principles are different but the effect is the same: concentration of a diluted analyte into a narrow zone and considerable increase of the method sensitivity. This review brings a survey of papers on electrophoretic sample stacking published approximately since the second quarter of 2014 till the first quarter of 2016. It is organized according to the principles of the stacking methods and includes chapters aimed at the concentration adjustment principle (Kohlrausch stacking), techniques based on pH changes, micellar methods, and other stacking techniques. Not reviewed are papers on transient ITP that are covered by another review in this issue.

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Chiral analysis of methorphan in opiate-overdose related deaths by using capillary electrophoresis

Cited by 19 publications

References 21 publications

Chiral selectors in CE: Recent development and applications (mid‐2014 to mid‐2016)

Chiral selectors in CE: Recent development and applications (mid‐2014 to mid‐2016)

Recent contributions for improving sensitivity in chiral CE

Recent progress of sample stacking in capillary electrophoresis (2014–2016)

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