Chip modularity enables molecular information access from organ-on-chip devices with quality control

Wang, Shang; Chen, Chen-Yu; Lo, Kimberly; Payne, Gregory F.; Bentley, William E.

doi:10.1016/j.snb.2019.05.030

Cited by 28 publications

(27 citation statements)

References 64 publications

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“…They are expected to provide more predictive pre-clinical tools than standard in vitro models [7][8][9]. To provide an accurate analysis of the stress imposed by the tested biomaterials, cells/tissues need to be surrounded by a range of (bio)sensors for real-time monitoring of inflammatory biomarkers [10,11]. Indeed, acute stress of biological tissues is accompanied by the secretion of direct (chemokines and cytokines) and non-direct inflammatory biomarkers [12].…”

Section: Introductionmentioning

confidence: 99%

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Chmayssem

Verplanck

Tanase

et al. 2021

Talanta

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There is a growing need for real-time monitoring of metabolic products that could reflect cell damages over extended periods. In this paper, we report the design and development of an original multiparametric (bio)sensing platform that is tailored for the real-time monitoring of cell metabolites derived from cell cultures. Most attractive features of our developed electrochemical (bio)sensing platform are its easy manufacturing process, that enables seamless scale-up, modular and versatile approach, and low cost. In addition, the developed platform allows a multiparametric analysis instead of single-analyte analysis. Here we provide an overview of the sensors-based analysis of four main factors that can indicate a possible cell deterioration problem during cell-culture: pH, hydrogen peroxide, nitric oxide/nitrite and lactate. Herein, we are proposing a sensors platform based on thick-film coupled to microfluidic technology that can be integrated into any microfluidic system using Luer-lock connectors. This platform allows obtaining an accurate analysis of the secreting stress metabolites during cell/tissues culture.

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Section: Introductionmentioning

confidence: 99%

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Chmayssem

Verplanck

Tanase

et al. 2021

Talanta

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“…A similar modular system was developed by Shang et al, who incorporated sensors with automated quality assurance testing to monitor sensor health and notify when the sensor required changing. [ 112 ] A summary of the microsensors that have been incorporated into organ‐on‐chip platforms can be found in Table 3 . By incorporating multiple sensors onto the same platform, a holistic view of cell health can be achieved.…”

Section: Functional Skin‐on‐chip Platformsmentioning

confidence: 99%

“…Modular Microfluidic Circuit: The concept of a modular microfluidic circuit with interchangeable OoCs and sensors have been explored; [111,112] however, a skin-specific system have yet to be achieved. Altering these circuits to suit the requirements of an SoC device will greatly enhance the functionality of skin models, automating the analysis procedure, and facilitating realtime monitoring of tissue health as well as enabling the user to select modules to meet their requirements.…”

Section: Conclusion and Future Outlookmentioning

confidence: 99%

Microfluidic Skin‐on‐a‐Chip Models: Toward Biomimetic Artificial Skin

Sutterby

Thurgood

Baratchi

et al. 2020

Small

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The role of skin in the human body is indispensable, serving as a barrier, moderating homeostatic balance, and representing a pronounced endpoint for cosmetics and pharmaceuticals. Despite the extensive achievements of in vitro skin models, they do not recapitulate the complexity of human skin; thus, there remains a dependence on animal models during preclinical drug trials, resulting in expensive drug development with high failure rates. By imparting a fine control over the microenvironment and inducing relevant mechanical cues, skin‐on‐a‐chip (SoC) models have circumvented the limitations of conventional cell studies. Enhanced barrier properties, vascularization, and improved phenotypic differentiation have been achieved by SoC models; however, the successful inclusion of appendages such as hair follicles and sweat glands and pigmentation relevance have yet to be realized. The present Review collates the progress of SoC platforms with a focus on their fabrication and the incorporation of mechanical cues, sensors, and blood vessels.

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“…Automated closed-loop monitoring and control of respiratory (oxygen), energy metabolism (glucose/lactate), acidification (pH), and other key functional metabolites would enable a large-scale culture of OOAC devices in pharmaceutical and toxicological testing environments while supporting regulatory and quality standards in regard to monitoring and recordkeeping. Several platforms for simple in situ monitoring of OOAC already exist [78][79][80]. Real-time, closed-loop control could trigger changes in environmental oxygen concentration or the addition of fresh culture media to replenish exhausted glucose/lactate levels.…”

Section: Automation and Closed-loop Controlmentioning

confidence: 99%

Translational Roadmap for the Organs-on-a-Chip Industry toward Broad Adoption

et al. 2020

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Organs-on-a-Chip (OOAC) is a disruptive technology with widely recognized potential to change the efficiency, effectiveness, and costs of the drug discovery process; to advance insights into human biology; to enable clinical research where human trials are not feasible. However, further development is needed for the successful adoption and acceptance of this technology. Areas for improvement include technological maturity, more robust validation of translational and predictive in vivo-like biology, and requirements of tighter quality standards for commercial viability. In this review, we reported on the consensus around existing challenges and necessary performance benchmarks that are required toward the broader adoption of OOACs in the next five years, and we defined a potential roadmap for future translational development of OOAC technology. We provided a clear snapshot of the current developmental stage of OOAC commercialization, including existing platforms, ancillary technologies, and tools required for the use of OOAC devices, and analyze their technology readiness levels. Using data gathered from OOAC developers and end-users, we identified prevalent challenges faced by the community, strategic trends and requirements driving OOAC technology development, and existing technological bottlenecks that could be outsourced or leveraged by active collaborations with academia.

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Chip modularity enables molecular information access from organ-on-chip devices with quality control

Cited by 28 publications

References 64 publications

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Microfluidic Skin‐on‐a‐Chip Models: Toward Biomimetic Artificial Skin

Translational Roadmap for the Organs-on-a-Chip Industry toward Broad Adoption

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