Chip based single cell analysis for nanotoxicity assessment

Shah, Pratikkumar; Kaushik, Ajeet; Zhu, Xuena; Zhang, Chengxiao; Li, Chen-Zhong

doi:10.1039/c3an02280c

Cited by 41 publications

(32 citation statements)

References 84 publications

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“…These methods make it possible to identify specific molecular targets and study the effects of nanoparticles on signaling pathways. The development of chip-based single-cell analysis is also of great interest for nanotoxicity assessment [71].…”

Section: Effect Of Nanoparticles On the Endoplasmic Reticulum And Golmentioning

confidence: 99%

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Maurizi¹,

Papa²,

Boudon³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

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The lack of toxicological data on nanomaterials makes it difficult to assess the risk related to their exposure, and as a result further investigation is required. This chapter presents the synthesis of controlled oxide nanoparticles followed by the evaluation of their safety profile or toxicity (iron, titanium and zinc oxides). The controlled surface chemistry, dispersion in several media, morphology and surface charge of these nanoparticles are presented (transmission electron microscopy, dynamic light scattering, zeta potential, X-ray photoelectron spectroscopy). Classical cytotoxic and cellular uptake studies on different cancer cell lines from liver, prostate, heart, brain and spinal cord are discussed. The incidence of nanoparticles on biogenesis and activity of cell organelles is also highlighted, as well as their biodistribution in animal models. The acute toxicity on zebrafish embryo model is also presented. Finally, the stress is put on the influence and the necessity of controlling the protein corona, a layer of plasma proteins physically adsorbed at the surface of such nanoparticles as a result of their presence in the bloodstream (or relevant biological fluids).

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Section: Effect Of Nanoparticles On the Endoplasmic Reticulum And Golmentioning

confidence: 99%

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Maurizi¹,

Papa²,

Boudon³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

View full text Add to dashboard Cite

show abstract

“…The EIS method is one of the best non-invasive analytical tool and is being explored for monitoring biological phenomena such as protein-protein interaction (Bogomolova et al 2009; Chang and Park 2010; Franks et al 2005), bio-sensing (Kaushik et al 2010), nano-toxicity (Shah et al 2014) and tumour drug interaction (Luongo et al 2013). An EIS based detection method, using 10 mM [Fe(CN) 6 ] 3−/4− as the redox probe in 50 mM PBS with 100 mM NaCl (pH 7.4)) at a potential of 0.30 V was developed to detect β-A.…”

Section: Advancements Of Electrochemical β-A Bio-sensingmentioning

confidence: 99%

Nano-biosensors to detect beta-amyloid for Alzheimer's disease management

Kaushik

Jayant

Tiwari

et al. 2016

Biosensors and Bioelectronics

Self Cite

151

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Beta-amyloid (β-A) peptides are potential biomarkers to monitor Alzheimer’s diseases (AD) for diagnostic purposes. Increased β-A level is neurotoxic and induces oxidative stress in brain resulting in neurodegeneration and causes dementia. As of now, no sensitive and inexpensive method is available for β-A detection under physiological and pathological conditions. Although, available methods such as neuroimaging, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) detect β-A, but they are not yet extended at point-of-care (POC) due to sophisticated equipments, need of high expertise, complicated operations, and challenge of low detection limit. Recently, β-A antibody based electrochemical immuno-sensing approach has been explored to detect β-A at pM levels within 30–40 minutes compared to 6–8 hours of ELISA test. The introduction of nano-enabling electrochemical sensing technology could enable rapid detection of β-A at POC and may facilitate fast personalized health care delivery. This review explores recent advancements in nano-enabling electrochemical β-A sensing technologies towards POC application to AD management. These analytical tools can serve as an analytical tool for AD management program to obtain bio-informatics needed to optimize therapeutics for neurodegenerative diseases diagnosis management

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“…101 In addition, studying nanotoxicity at the single cell level is also critical in establishing toxicological profiles quantitatively. 106 3.2.2. The cell cycle is also one of the crucial factors underlying nanotoxicity and hence nanotoxicology.…”

Section: Mechanisms Of Nanotoxicitymentioning

confidence: 99%

Toxicity of engineered metal oxide nanomaterials mediated by nano–bio–eco–interactions: a review and perspective

Aker

et al. 2015

Environ. Sci.: Nano

100

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Along with the expanding use of engineered metal oxide nanomaterials (MONMs), there is a growing concern over their unintentional adverse toxicological effects on human health and the environment upon release and exposure. It is inevitable that biota will be exposed to nanomaterials, through intentional administration or inadvertent contact under such circumstances. Therefore, a thorough investigation of the potential nanotoxicity of MONMs at the nano-bio-eco interface is urgently needed. In general, nanomaterials interact with their surrounding environments, biotic and abiotic, immediately upon introduction into the environment. The behavior and fate of MONMs are influenced by the dynamics of the environment. Thus, understanding the interactions at the nano-bio-eco interface is necessary for selecting and designing MONMs with minimum adverse impacts. Despite the limitations of currently available techniques, careful characterization of nanomaterials and the choosing of methodologies that promote further risk assessment promise more reliable and accurate data output. Conventional toxicological analysis techniques lack the power to handle the large datasets generated from in vitro/in vivo observations. This paper provides a comprehensive review of the recent experimental and theoretical studies on the toxicity of MONMs mediated by two-way or three-way interactions. In the Perspectives, we also call for more open collaborations between industry, academia, and research labs to facilitate nanotoxicological studies focused specifically on interactions at the nano-bio-eco interface, leading to safe and effective nanotechnology for commercial, environmental, and medicinal use. Environ. Sci.: Nano This journal isThe behavior and fate of metal oxide nanomaterials (MONMs) are influenced by the dynamic interactions among the different compartments in natural environments. Thus, understanding the interactions at the nano-bio-eco interface is necessary for selecting and designing MONMs with minimum adverse impacts. This paper provides a comprehensive review of the recent experimental and theoretical studies on the toxicity of MONMs mediated by two-way or three-way interactions. In the Perspectives, we also call for more open collaborations between industry, academia, and research labs to facilitate nanotoxicological studies focused specifically on interactions at the nano-bio-eco interface, leading to safe and effective nanotechnology for commercial, environmental, and medicinal use.

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Chip based single cell analysis for nanotoxicity assessment

Cited by 41 publications

References 84 publications

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Nano-biosensors to detect beta-amyloid for Alzheimer's disease management

Toxicity of engineered metal oxide nanomaterials mediated by nano–bio–eco–interactions: a review and perspective

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