Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis

Bäcker, Matthias; Rakowski, D.; Poghossian, Arshak; Biselli, M.; Wagner, Patrick; Schöning, Michael J.

doi:10.1016/j.jbiotec.2012.03.014

Cited by 52 publications

(26 citation statements)

References 18 publications

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“…Although previous studies have also used microfluidics for glutamate detection, the cells were spatially removed from the electrode in these cases. 23 Here, the cells and sensor were brought in close proximity within the bioreactor. The low limit of quantitation of the glutamate sensor combined with the small volume of the μCA bioreactor (V = 26 μL) allowed for monitoring small changes in glutamate such as those seen in synaptic signaling.…”

Section: Discussionmentioning

confidence: 99%

“…17 Just within the subcategory of oxidase enzymes, glucose-, lactate-, choline-, cholesterol-, and glutamateoxidase have all been used for electrochemical detection. [17][18][19] Other benefits to enzymatic sensors include their biocompatibility, portability, compatibility, and relative low cost. Screen printing is an inexpensive and reliable way to fabricate electrodes that, when enzymatically modified, are also sensitive and selective.…”

mentioning

confidence: 99%

“…17,22 In addition, microfluidics allow for low sample volumes (26 μL sample chamber), enhancing the ability to detect small metabolic changes. 5,23 This versatile platform can easily integrate cells or other enzyme sensors to monitor cellular bioenergetics in real-time. 17 In addition to increasing the analytical power, microfluidic systems also help recapitulate physiological conditions.…”

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confidence: 99%

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Electrochemical Microphysiometry Detects Cellular Glutamate Uptake

Miller

McClain

Cliffel

2018

J. Electrochem. Soc.

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Glutamate is the principal excitatory amino acid in the vertebrate nervous system and is responsible for learning and memory. Understanding of these complex biological processes can be gained through experimentally accessible systems of glutamate detection. In this work, the microclinical analyzer (μCA) was used with a sensitive and stable glutamate sensor and model neuronal cells for quantitative glutamate detection under physiological relevant shear. Glutamate was detected by immobilized glutamate oxidase on a screen-printed electrode array. The sensor's linear range spanned glutamate's physiological to pathophysiological concentration range, and the biologically relevant sub-second to month temporal range. After 11 hours of use, the sensor retained 91 ± 1% of its signal, and it was able to be stored for a month without a significant decrease. When model neuronal cells were integrated into the μCA bioreactor and exposed to glutamate, they initially took up 210 ± 100 μmoles of glutamate, which increased to 390 ± 50 μmoles during their second exposure. These data suggest that the neurotransmitter uptake systems were functional and may be upregulated. The dynamic and durable μCA platform offers an experimentally accessible system of glutamate detection that can be used to monitor glutamate metabolism and signaling. Glutamate is one of the 20 canonical amino acids that together provide the structural and enzymatic foundation of proteins. Alone, glutamate plays a very different role and is itself an excitatory signaling molecule widely distributed throughout the central nervous system. In fact, glutamate is the most prevalent neurotransmitter and its function is essential for proper neurocognition, learning, and memory. Overactivation of glutamate receptors causes excitotoxicity, a pathological process whereby neurons are damaged or killed, which may result in neurodegeneration.1,2 Because of its central role in metabolic and cognitive processes, a strong effort has been put toward developing methods to detect glutamate. [2][3][4][5] Accurate detection of glutamate can be accomplished using many techniques, including spectrometry, spectroscopy, and electrochemistry. The benefits of spectrometry and spectroscopy include sensitivity and selectivity. However, mass spectrometry requires chromatographic separation or vacuum preparation, which decreases the temporal resolution of the system. 4 Although spectroscopic techniques, such as the iGluSNFR, 2 offer impressive temporal resolution, they require optical transparency thereby limiting the scope of samples that can be analyzed. In contrast, electrochemical sensors require almost no sample manipulation and can be placed directly in the area of interest, allowing, for example, detection of glutamate and dopamine signaling in the brain.6-8 Electrochemical sensors are also versatile: They can be made on the nanoscale, 1 have been 3D-printed, 9 and can be inexpensive.10,11 The highly translatable nature of electrochemical techniques has already been demonstrated with the a...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Electrochemical Microphysiometry Detects Cellular Glutamate Uptake

Miller

McClain

Cliffel

2018

J. Electrochem. Soc.

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“…This can be done by using flow injection analysis (FIA) (Backer et al, 2013;Hernandez et al, 2013;Ito et al, 2011;Y. Wang et al, 2010b;Yi et al, 2012).…”

Section: Flow Injectionmentioning

confidence: 99%

“…Another interesting application field is the monitoring of nutrients and metabolic products in cell cultures and biotechnological processes. In this context, important analytes are glucose (Ges and Baudenbacher, 2010a;Rodrigues et al, 2008), lactate (Cheng et al, 2006), but also glutamine and glutamate (Backer et al, 2013).…”

Section: Applicationsmentioning

confidence: 99%

Microfluidic enzymatic biosensing systems: A review

Mross

Pierrat

Zimmermann

et al. 2015

Biosensors and Bioelectronics

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Advances in sensor developments for cell culture monitoring

Kim,

Yeo

2023

BMEMat

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Cell culture encompasses procedures for extracting cells from their natural tissue and cultivating them under controlled artificial conditions. During this process, various factors, including cell physiological/morphological properties, culture environments, metabolites, and contaminants, have to be precisely controlled and monitored for the survival of cells and the pursuit of the desired properties of the cells. This review summarizes recent advances in sensor technologies and manufacturing strategies for various cell culture platforms using traditional plastics, microfluidic chips, and scalable bioreactors. We share the details of newly developed biological sensors, chemical sensors, optical sensors, electronic chip technologies, and material integration methods. The precise control of parameters based on the feedback by these sensors and electronics enhances cell culture quality and throughput.

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Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis

Cited by 52 publications

References 18 publications

Electrochemical Microphysiometry Detects Cellular Glutamate Uptake

Electrochemical Microphysiometry Detects Cellular Glutamate Uptake

Microfluidic enzymatic biosensing systems: A review

Advances in sensor developments for cell culture monitoring

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