Chinese medicine CGA formula ameliorates liver fibrosis induced by carbon tetrachloride involving inhibition of hepatic apoptosis in rats

Tian, Huayu; Liu, Lin; Li, Zhixiong; Liu, Wei; Sun, Zhaolin; Xu, Yongbin; Wang, Shunchun; Liang, Chungeng; Hai, Yamei; Zhao, Yu; Hu, Yiyang; Peng, Jinghua

doi:10.1016/j.jep.2018.11.027

Cited by 21 publications

(11 citation statements)

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“…Importantly, the balance of proapoptotic Bax, Bak, and antiapoptotic Bcl-2 proteins in mitochondria determine the fate of cells. Tian et al demonstrated that the protein expression of Bcl-2 in the liver tissue was downregulated, while levels of Bak and Bax were upregulated in rats with CCl 4 stimulation [ 7 ]. In the present study, RSV treatment largely decreased the expression levels of ERS-related proteins CHOP; BIP; cleaved caspase-3, caspase-7, and caspase-12; Bax; and Bak while promotes the expression of anti-apoptosis protein Bcl2, indicating that RSV is protective in ERS-induced apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Pathological stimuli, including inflammation and oxidative stress, can disrupt the function of the ER, resulting in the aggregation of misfolded proteins in the ER cavity, which causes cell homeostasis imbalance and hepatic dysfunction [ 5 ]. Preclinical studies have suggested that upregulation of ER stress (ERS)-related proteins, including binding immunoglobulin protein (BIP), Bcl-2 associated X protein (Bax), Bcl-2 homologous antagonist/killer (Bak), and C/EBP homologous protein (CHOP), occurs in animal models of HF [ 6 , 7 ]. CHOP may lead to HF by increasing the expression of fibrotropic factors, including α-smooth muscle actin (α-SMA), TGF-β, and collagen [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Resveratrol Alleviates Hepatic Fibrosis in Associated with Decreased Endoplasmic Reticulum Stress-Mediated Apoptosis and Inflammation

Sheng

et al. 2022

Inflammation

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Abstract—Hepatic fibrosis (HF) is the typical response to chronic liver disease and is characterized by deposition of abundant extracellular matrix. The aim of the present study was to investigate the protective effect of resveratrol (RSV) in a CCl4-induced rat model of HF. We demonstrate that the administration of RSV effectively improves liver function and ameliorates liver fibrosis by reducing collagen deposition and reversing the expression of COL1A1 and PPAR-γ. Treatment efficacy of RSV could be attributed to reversed epithelial-mesenchymal transition progress with upregulated expression of E-cadherin and downregulated N-cadherin, vimentin, and α-SMA. Moreover, RSV significantly decreased the levels of endoplasmic reticulum stress (ERS)-related proteins CHOP; Bip; cleaved caspase-3, caspase-7, and caspase-12; Bax; and Bak while promotes the expression of anti-apoptosis protein Bcl2. The important role of ERS in HF was confirmed by using 4-PBA, an ERS inhibitor, which markedly ameliorated CCl4-induced HF. Further, mechanistic studies demonstrated that RSV significantly decreased CCl4-induced transforming growth factor-β synthesis and inflammatory factor (tumor necrosis factor-α and interleukin-6) expression and reduced the inflammation of hepatic stellate cells by inhibiting the NF-κB pathway in vivo and in vitro. In conclusion, the results suggested that RSV ameliorated HF in associated with decreased ERS-induced apoptosis and inflammation in rats.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Resveratrol Alleviates Hepatic Fibrosis in Associated with Decreased Endoplasmic Reticulum Stress-Mediated Apoptosis and Inflammation

Sheng

et al. 2022

Inflammation

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“…FZHY extracts were analyzed by ultra-high-performance liquid chromatography-Q exactive hybrid quadrupole orbitrap high-resolution accurate mass spectrometric (UHPLC-Q-Orbitrap HRMS, Thermo Fisher Scientific Inc., Grand Island, NY, USA) [1] and standards of the components in FZHY identified previously [6], [7], [8], [9]. FZHY extracts contain adenosine (5.21 mg/g), amygdalin (5.31 mg/g), salvianolic acid b (18.22 mg/g) and deoxyschizandrin (2.62 mg/g) (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Data format Raw Experimental factors Before mass spectroscopy, FZHY extracts and gypenosides were dissolved by methanol. CS-PS was hydrolyzed by trifluoroacetic acid, then was vacuum dried and dissolved in methanol.Experimental features Samples were prepared and analyzed according to the protocol described previously [1], [4], [5] . Data source location Shanghai, China Data accessibility Data is with this article Related research article Huajie Tian, Lin Liu, Zhixiong Li, Wei Liu, Zhaolin Sun, Yongbin Xu, Shunchun Wang, Chungeng Liang, Yamei Hai, Qin Feng, Yu Zhao, Yiyang Hu, Jinghua Peng.…”

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confidence: 99%

The components data of fuzheng huayu extracts, cordyceps sinensis mycelia polysaccharide, gypenosides and amygdalin

Liu

Sun

et al. 2019

Data in Brief

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Fuzheng Huayu (FZHY) capsule is a traditional Chinese medicine composed of six Chinese medicinal herbs Tian et al. [1] and approved by China food and drug administration for liver fibrosis treatment [2] , [3] Liu et al., 2009 and Liu et al., 2005. CGA formula consisting of Cordyeps sinensis polysaccharide (CS-PS), gypenosides (G), and amygdalin (A), are derived from FZHY formula. It is necessary to identify the chemical profile of FZHY and CGA formula to describe the mechanisms and the corresponding components of anti-fibrosis. It is showed that FZHY contains adenosine (5.21 mg/g), amygdalin (5.31 mg/g), salvianolic acid b (18.22 mg/g) and deoxyschizandrin (2.62 mg/g), respectively. CS-PS contained 60.5 ± 2.2% total carbohydrate, including 14.17% arabinose, 25.35% glucose and 60.48% galactose. Gypenosides contain 10.34% gypenosides XLIX and 16.58% gypenosides A. These data provide the primary chemical profile of FZHY and CGA formula and an example for components analysis of traditional Chinese medicine.

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“…Also, vitamin E is reported to ameliorate several types of liver diseases in patients with non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), hepatic fibrosis, inflammation and hepatocellular ballooning (Sanyal et al, 2010;Sato et al, 2015). Additionally, vitamin E has been used in animal models of many liver diseases to significantly attenuate NAFLD (Oliveira et al, 2003), NASH (Zamin et al, 2010), CCl4 induced hepatic fibrosis (Tian et al, 2019), and MSGinduced oxidative stress and liver injury enzymes (Onyema et al). However, vitamin E has not been used before to protect the kidney ultrastructure upon MSG intoxication in an animal model.…”

Section: Introductionmentioning

confidence: 99%

Suppression of Monosodium Glutamate-Induced Acute Kidney Injury and Renal Ultrastructural Damage in Rats by Vitamin E

et al. 2019

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Food additives and flavour enhancers used in the food industry are potential health risks. We tested the hypothesis that the food additive and flavour enhancer, monosodium glutamate (MSG), which is the sodium salt of glutamic acid can induce ultrastructural alterations to the kidney, and the antioxidant vitamin E can protect against acute kidney injuries induced by a toxic dose of MSG in a rat model of the disease. The model group of rats received a daily dose of MSG (4 gm/kg) for 7 days, whereas the protective groups were either received a 100 mg/kg vitamin E plus MSG or 300 mg/kg vitamin E plus MSG for 7 days. Rats were then sacrificed on day 8. Transmission and light microscopy images revealed substantial kidney damage induced by MSG in the model group as demonstrated by degenerated epithelial cells with Pyknotic nuclei, swollen mitochondria, damaged brush margins, dilated tubules, and widening of Bowman's space with shrinkage and deformity of some glomeruli. Treatment of the model group with vitamin E showed a substantial protection of kidney tissue and renal ultrastructure by 300 mg/kg vitamin E compared to a partial protection by 100 mg/kg vitamin E. In addition, MSG significantly (p<0.05) increased serum levels of urea and creatinine, which were significantly (p<0.05) decreased with vitamin E. However, for serum creatinine, high doses of vitamin E (300 mg/kg) were more effective than lower doses (100 mg/kg) of vitamin E. These results indicate that vitamin E at 300 mg/kg effectively protects against MSG-induced acute kidney injury in rats.

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Chinese medicine CGA formula ameliorates liver fibrosis induced by carbon tetrachloride involving inhibition of hepatic apoptosis in rats

Cited by 21 publications

References 50 publications

Resveratrol Alleviates Hepatic Fibrosis in Associated with Decreased Endoplasmic Reticulum Stress-Mediated Apoptosis and Inflammation

Resveratrol Alleviates Hepatic Fibrosis in Associated with Decreased Endoplasmic Reticulum Stress-Mediated Apoptosis and Inflammation

The components data of fuzheng huayu extracts, cordyceps sinensis mycelia polysaccharide, gypenosides and amygdalin

Suppression of Monosodium Glutamate-Induced Acute Kidney Injury and Renal Ultrastructural Damage in Rats by Vitamin E

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