Chinese border disease virus strain JSLS12-01 infects piglets and down-regulates the antibody responses of classical swine fever virus C strain vaccination

Li, Mao; Li, Wenliang; Liu, Xia; Fei, Hao; Yang, Leilei; Deng, Jiawu; Zhang, Wenwen; Wei, Jinbei; Ji, Jiang

doi:10.1016/j.vaccine.2015.06.059

Cited by 5 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and seroconversion from 14 days p.i. ; however, no clinical signs or pathological changes were observed [33]. These data, together with that from previous studies [10] strongly indicate that BDV grows very efficiently in sheep cells, but BDV isolates, even Gifpig isolated from piglets, cannot easily adapt to primary pig kidney cells in culture (Fig.…”

Section: Discussionsupporting

confidence: 77%

Identification of specific amino acid residues in the border disease virus glycoprotein E2 that modify virus growth in pig cells but not in sheep cells

Fahnøe

Deng

Davids

et al. 2020

Journal of General Virology

View full text Add to dashboard Cite

Border disease virus (BDV) envelope glycoprotein E2 is required for entry into cells and is a determinant of host tropism for sheep and pig cells. Here, we describe adaptive changes in the BDV E2 protein that modify virus replication in pig cells. To achieve this, two BDV isolates, initially collected from a pig and a sheep on the same farm, were passaged in primary sheep and pig cells in parallel with a rescued variant of the pig virus derived from a cloned full-length BDV cDNA. The pig isolate and the rescued virus shared the same amino acid sequence, but the sheep isolate differed at ten residues, including two substitutions in E2 (K771E and Y925H). During serial passage in cells, the viruses displayed clear selectivity for growth in sheep cells; only the cDNA-derived virus adapted to grow in pig cells. Sequencing revealed an amino acid substitution (Q739R) in the E2 domain DA of this rescued virus. Adaptation at the same residue (Q739K/Q739R) was also observed after passaging of the pig isolate in sheep cells. Use of reverse genetics confirmed that changing residue Q739 to R or K (each positively charged) was sufficient to achieve adaptation to pig cells. Furthermore, this change in host tropism was suppressed if Q739R was combined with K771E. Another substitution (Q728R), conferring an additional positive charge, acquired during passaging, restored the growth of the Q739R/K771E variant. Overall, this study provided evidence that specific, positively charged, residues in the E2 domain DA are crucial for pig-cell tropism of BDV.

show abstract

Section: Discussionsupporting

confidence: 77%

Identification of specific amino acid residues in the border disease virus glycoprotein E2 that modify virus growth in pig cells but not in sheep cells

Fahnøe

Deng

Davids

et al. 2020

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…This combination of pathogens is particularly costly to the Chinese pig industry, because PRRSV is immunosuppressive it seriously inhibits the immune response to the CSF vaccine. Further reports have shown that two other Pestiviruses , BVDV and BDV, strongly inhibit the immune response of vaccine against CSFV (31, 32). Based on a coinfection model for PCV2 and CSFV, bioinformatic analyses indicated that mitochondrial dysfunction, nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated oxidative stress response and apoptosis signaling pathways might be the specific targets during PCV2-CSFV coinfection (33).…”

Section: Epidemiological Characteristicsmentioning

confidence: 99%

“…Subgenotype 2.1c is also distributed in Thailand and Laos. Tu et al (53) showed that subgenotype 2.1c appeared in Guangxi as early as 1998 and Peng et al (57) further divided 15 isolates of subtype 2.1 from Guangdong in 2011 into three subgenotypes of 2.1b, 2.1c, and 2.1d, while the prevalence of 2.1c and 2.1d was the first reported in Guangdong (32, 58). The distribution of genetic diversity is probably related to the transportation of pigs and the level of development of the pig industries.…”

Section: Geographical Distribution Of Genotypesmentioning

confidence: 99%

Classical Swine Fever in China-An Update Minireview

Zhou

2019

Front. Vet. Sci.

View full text Add to dashboard Cite

Classical swine fever (CSF) remains one of the most economically important viral diseases of domestic pigs and wild boar worldwide. The causative agent is CSF virus, it is highly contagious, with high morbidity and mortality rates; as such, it is an OIE-listed disease. Owing to a nationwide policy of vaccinations of pigs, CSF is well-controlled in China, with large-scale outbreaks rarely seen. Sporadic outbreaks are however still reported every year. In order to cope with future crises and to eradicate CSF, China should strengthen and support biosecurity measures such as the timely reporting of suspected disease, technologies for reliable diagnoses, culling infected herds, and tracing possible contacts, as well as continued vaccination and support of research into drug and genetic therapies. This mini-review summarizes the epidemiology of and control strategies for CSF in China.

show abstract

“…Interestingly, animals with positive PRRSV antibody have been also found positive for CSFV antibody, i.e., 13.33% serum samples showed the presence of PRRSV and CSFV. The presence of PRRSV can also have profound effect on the vaccination efficiency because PRRSV is immunesuppressive and it seriously inhibits the immune response to CSF vaccine (Tao et al, 2013;Mao et al, 2015;Zhou, 2019). During the study period, Kolasib districts of Mizoram showed the highest prevalence (34.69%) of PRRSV antibodies as compared to other three districts of Mizoram, i.e., Aizawl (25.00%), Champhai (17.9%) and Serchhip (34.5%) as shown in Fig.…”

Section: Resultsmentioning

confidence: 92%

Seroprevalence of porcine reproductive and respiratory syndrome virus and classical swine fever virus in pigs of Mizoram, India

Lalhruaipuii¹,

Shakuntala²,

Sen³

2020

JEB

View full text Add to dashboard Cite

This study aimed to evaluate the prevalence of antibodies against two economically significant viral pathogens in domestic pigs like porcine reproductive and respiratory syndrome virus (PRRSV) and classical swine fever virus (CSFV) through a serological study in swine herds in Mizoram, India. Serum samples were collected from apparently healthy and suspected pigs of different age groups comprising weaners, growers and adult in four districts (Aizawl, Champhai, Kolasib and Serchhip) of Mizoram during February, 2018 to June, 2019. Seroprevalence of CSFV and PRRSV specific antibodies was detected by enzyme-linked immunosorbent assay (ELISA). Out of 420 samples, 117 samples were found positive for PRRSV, 171 samples for CSFV indicating a mean positivity of 27.86% and 40.48%. Presence of mixed infection was observed in 13.33% samples. The present study demonstrated the extensive circulation of classical swine fever virus and porcine reproductive and respiratory syndrome virus among domestic pig populations of Mizoram, India.

show abstract

Chinese border disease virus strain JSLS12-01 infects piglets and down-regulates the antibody responses of classical swine fever virus C strain vaccination

Cited by 5 publications

References 33 publications

Identification of specific amino acid residues in the border disease virus glycoprotein E2 that modify virus growth in pig cells but not in sheep cells

Identification of specific amino acid residues in the border disease virus glycoprotein E2 that modify virus growth in pig cells but not in sheep cells

Classical Swine Fever in China-An Update Minireview

Seroprevalence of porcine reproductive and respiratory syndrome virus and classical swine fever virus in pigs of Mizoram, India

Contact Info

Product

Resources

About