Chill sensitivity and cryopreservation of eggs of the greater wax moth Galleria mellonella (Lepidoptera: Pyralidae)

Permeability Barriers to Embryo Cryopreservation of <I>Pectinophora gossypiella</I> (Lepidoptera: Gelechiidae)

“…This barely allows for sufÞcient dehydration of the embryos, making cryopreservation feasible. Even though this study reports a signiÞcantly higher hatch percentage (9 Ð 15% viable embryos, postvitriÞcation) than previous reports among other lepidopterans (Luo et al 2006;Roversi et al 2008), the results are accompanied by large standard deviations. The current procedure seems to be affected by numerous factors, including very high dispersion among the embryonic stages.…”

“…However, Cosi et al (2010) reported that, in the case of G. mellonella, detergents were as effective as heptane and resulted in a much higher survival because of the elimination of solvent toxicity. However, Roversi et al (2008) had used Tween 80 and reported a postcryopreservation hatch percentage of 1.6 Ϯ 0.5%. Although sufÞcient to rejuvenate a healthy colony of waxmoths, the hatch percentage is signiÞcantly lower than previously reported studies on insects.…”

“…Roversi et al (2008) and Cosi et al (2010) used Tween 80 to permeabilize the eggshell of G. mellonella, and Rand et al (2010) assessed the efÞcacy of phytosurfactants on Drosophila embryos. In the current study, p-tert-octylphenoxy polyethoxyethanol (Triton X-100) in water and propane 1,2-diol were used to permeabilize the Pectinophora embryos.…”

“…Although they reported hatching of larvae, none of the larvae survived. Roversi et al (2008) cryopreserving the embryos of the wax moth Galleria mellonela (L.) reported a hatch percentage of 1.6 Ϯ 0.5% immediately after retrieving from liquid nitrogen. Cosi et al (2010), cognizant of the above lower hatch percentage in G. mellonella, reported on further assessments using Tween 80 and sodium hydroxide.…”

Novel staining procedures for whole mount preparations of greater wax moth (Galleria mellonella) embryos: toluidine blue-rhodamine B, plus calcofluor white

“…Cold storage of eggs and embryos is a promising strategy for avoiding the cost of the continuous rearing of new organisms while preventing genetic drift. Development of new approaches requires accurate determination of embryonic stages, because different stages respond differently to cryoprotectants and cooling (Roversi et al 2008;Rajamohan et al 2015). One insect pest of interest is the greater wax moth, Galleria mellonella.…”